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http://dx.doi.org/10.4489/KJM.2003.31.3.161

The Immuno-Modulatory and Antitumor Effects of Crude Polysaccharides Extracted from Daedaleopsis tricolor  

Shim, Sung-Mi (Department of Biology, University of Incheon)
Im, Kyung-Hoan (Department of Biology, University of Incheon)
Kim, Jung-Wan (Department of Biology, University of Incheon)
Lee, U-Youn (Department of Biology, University of Incheon)
Kim, Ha-Won (Department of Life Science, University of Seoul)
Lee, Min-Woong (Department of Applied Biology, Dongguk University)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
The Korean Journal of Mycology / v.31, no.3, 2003 , pp. 161-167 More about this Journal
Abstract
Neutral salt soluble [0.9% NaCl (Fr. NaCl)], hot water soluble (Fr. HW) and methanol soluble (Fr. MeOH) materials were extracted from Daedaleopsis tricolor. In vitro cytotoxicity tests, Fr. HW was not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2 and HT-29 at the concentration of $0{\sim}2,000\;{\mu}g/ml$, while Fr. NaCl and Fr. MeOH were cytotoxic to the cell lines. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 77.4% in mice inoculated with Sarcoma 180. Fr. NnCl and Fr. HW improved proliferation of spleen cells and the immunopotentiation activity of B lymphocyte by increasing spleen cells and the alkaline phosphatase activity by $1.7{\sim}2.4\;and\;2.2{\sim}8.7$ folds, respectively. Fr, NaCl generated $90\;{\mu}M$ of nitric oxide (NO) when cultured with RAW 264.7 at the concentration of $50\;{\mu}g/ml$, while lipopolysaccharide, a positive control, produced $79{\mu}M$. Intraperitoneal injection with Fr. NaCl (50 mg/kg body weight) increased the numbers of peritoneal exudate cells and circulating leukocytes by 10 folds and two folds, respectively, than in the control group. The antitumor effect of D, tricolor was likely due to immunopotentiation activity.
Keywords
Antitumor; Crude polysacchrides; Daedaleopsis tricolor; Immuno-modulatory effect;
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  • Reference
1 Host-mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune /
[ Komatsu, N.;Okubo, S.;Kikumoto, S.;Kimura, K.;Saito, G. ] / Gann.
2 In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts /
[ Mossman, B.T. ] / Environ. Health Perspect.   DOI
3 Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens /
[ Ohno, N.;Arai, Y.;Suzuki, I.;Yadomae, T. ] / J. Phamacobio-Dyn.   DOI
4 Calvacine, a new antitumor agent /
[ Roland, J.E.;Chmielewicz, Z.E.;Weiner, B.A.;Gross, A.M.;Boenong, O.P.;Luck, J.V.;Bardos, T.J.;Redly, H.C.;Sugiura, K.;Stock, C.C.;Lucas, E.H.;Byerrum, R.U.;Stevens, J.A. ] / Science   DOI
5 Cooperative role of T lymphocytes and macrophages in antitumor activity of mice pretreated with schizophyllan /
[ Suzuki, M.;Arika, T.;Amemiya, T.;Fujiwara, M. ] / Jpn. J. Exp. Med.
6 Protein-bound polysaccaride preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AH-13 by oral use /
[ Tsugagoshi, S.;Ohashi, E. ] / Gann
7 Noncytotoxic, antitumor polysaccharides;Advances in Carbohydrate Chemistry and Biochemistry, Vol 32 /
[ Whistler, R.L.;Bushway, A.A.;Singh, P.P.;Nakahara, W.;Tokuzen, R.;Tipson, R.S.(ed.);Horton, D.(ed.) ] /
8 한국산 영지의 무기 성분 및 면역 증강 작용에 관한 연구 /
[ 신혜원;김하원;최웅칠;도상학;김병각 ] / 생약학회지
9 신령버섯(Agaricus blazei Murill)에서 분리한 다당체의 Staphylococcus aureus 감염 및 마우스 Sarcoma 180 종양세포에 대한 방어효과 /
[ 구현옥;김인천;박신자;정상희;박종명;이재진;유한상;이영순 ] / 힌국실험동물학회지
10 버섯추출물의 항산화 활성에 관한 연구 /
[ 박상신;유국현;민태진 ] / 한국균학회지
11 Fomitella fraxinea로부터 분리한 면역활성 다당류(I)- 중성염 용액 추출 다당류의 특성 /
[ 조수묵;이재훈;한상배;김환묵;유승헌;유익동 ] / 한국균학회지
12 Fomitella fraxinea로부터 분리한 면역활성 다당류(II)- 열수추출 다당류의 분리 및 특성 /
[ 조수묵;이재훈;한상배;김환묵;유승헌;유익동 ] / 한국균학회지
13 영지의 균사체성 <TEX>${\beta}-glucan$</TEX>에 의한 Raw 264.7 대식세포의 Nitric Oxide 생성 /
[ 한만덕;이은숙;김영권;이중우;정훈;윤경하 ] / 한국균학회지
14 キノコの化學?生物學 /
[ 水野 卓;川合正允 ] /
15 Textbook of medical physiology /
[ Arthur, C.;Guyton, M.D. ] /
16 Antitumor polysaccharide derived chemically from natural glucan (Pachman) /
[ Chihara, G.;Hamuro, G.;Meada, Y.;Arai, Y.;Fukoka, F. ] / Nature
17 Inhibition of mouse Sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) Sing /
[ Chihara, G.;Maeda, Y.;Hamuro, J.;Sasaki, T.;Fukuoka, F. ] / Nature   DOI   ScienceOn
18 Effect of various extrinsic and intrinsic factors on the nitric oxide production of murine macrophage /
[ Choi, S.H.;Jun, C.D.;Lee, B.S.;Park, S.D.;Oh, J.S.;Chung, H.T. ] / Kor. J. BRM
19 Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability /
[ Denizot, F.;Lang, R. ] / J. Immunol. Methods   DOI   ScienceOn
20 Role of nutrition in immunologic function /
[ Gross, R.L.;Newbeme, P.M. ] / Physiol. Rev.
21 <TEX>$INF-{\beta}$</TEX> induced l-arginine-dependent toxoplasmastic activity in murine peritoneal macrophages is mediated by endogenous tumor necrosis <TEX>$factor-{\alpha}$</TEX> /
[ Jan, A.M.;Marielle, E.B.;Peter, H.N.;Pieter, S.H.;Ralph, V.E. ] / J. Immunol.
22 Preparation and immunochemical characterization of antibody of branched <TEX>${\beta}-(1{\to}3)-D-glucan$</TEX> of Volvariella volvacea and its use in studies of antitumor actions /
[ Kishida, E.;Sone, Y.;Shibata, S.;Misaki, A. ] / Agric. Biol. Chem.
23 Anticomplementary activity of antitumor polysacchrides /
[ Sugihara, T.;Yoshioka, Y.;Nishioka, K. ] / Nature New Biology   DOI
24 잣버섯 성분의 면역세포 및 전사인자의 활성화 작용에 관한 연구 /
[ 진미림 ] / 대울대학교 대학원 논문집