Browse > Article
http://dx.doi.org/10.4489/KJM.2003.31.3.155

Studies on Immuno-Modulatory and Antitumor Effects of Crude Polysaccharides Extracted from Paecilomyces sinclairii  

Shim, Sung-Mi (Department of Biology, University of Incheon)
Im, Kyung-Hoan (Department of Biology, University of Incheon)
Lee, U-Youn (Department of Biology, University of Incheon)
Kim, Jung-Wan (Department of Biology, University of Incheon)
Shim, Mi-Ja (Department of Life Science, University of Seoul)
Lee, Min-Woong (Department of Applied Biology, Dongguk University)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
The Korean Journal of Mycology / v.31, no.3, 2003 , pp. 155-160 More about this Journal
Abstract
Neutral salt soluble [0.9% NaCl (Fr. NaCl)], hot water soluble (Fr. HW) and methanol soluble (Fr. MeOH) materials were extracted from Paecilomyces sinclairii. In vitro cytotoxicity tests. Fr. HW was not cytotoxic against MH3T3, Sarcoma 180 and HepG2 cancer cell lines at the concentration of $0{\sim}2,000\;{\mu}g/ml$, while HT-29 cell line was cytotoxic at the concentration of $100\;{\mu}g/ml$. Fr. NaCl and Fr. MeOH were slightly cytotoxic to the cell lines. Intraperitoneal injection with Fr. NaCl and Fr. MeOH exhibited antitumor activity with life prolongation effect of 32.3% in mice inoculated with Sarcoma 180. Fr. NaCl improved proliferation of spleen cells and the immunopotentiation activity of B lymphocyte by increasing spleen cell numbers and the alkaline phosphatase activity by $2.4{\sim}2.6\;and\;2.7{\sim}3.9$ folds, respectively. Fr. NaCl generated $89\;{\mu}M$ of nitric oxide (NO) when cultured with RAW 264.7, a mouse macrophage cell line, at the concentration of $50\;{\mu}g/ml$, while iipopolysaccharide, a positive control, produced $79\;{\mu}M$. The Fr. NaCl showed the hightest antitumor effect and activation of B lymphocytes and macrophage. From these results, antitumor effect of P. sinclaitii was likely due to immunopotentiation activity.
Keywords
Antitumor; Crude polysacchrides; Immuno-modulatory effect; Paecilomyces sinclairii;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(II)-융합균사체의 항암성분이 생쥐의 면역세포에 미치는 영향 /
[ 김병각;문철;김채균;윤종명;심미자;김하원;최웅칠 ] / 생약학회지
2 매미눈꽃 동충하초의 균사 생장과 자실체 형성 조건의 특성 /
[ 심성미;이경림;임경환;이우윤;이민웅;이태수 ] / 한국균학회지
3 띠미로버섯 추출물의 항암효과 /
[ 양규호;양정희;류병호 ] / Kor. J. Appl. Microbiol. Biotechnol.
4 팽나무버섯의 항암성분에 관한 연구 /
[ 우명식 ] / 한국균학회지
5 운지버섯균사체 추출물의 항암효과에 관한 연구 /
[ 이병우;이명섭;박기문;김창한;안평욱;최춘언 ] / 한국산업미생물학회지
6 상황버섯 자실체의 in vitro 및 in vivo 항암활성 /
[ 이영경;한명주;박순영;김동현 ] / Kor. J. Food Sci. Technol.
7 Fomitella fraxinea로부터 분리한 면역활성 다당류(I)- 중성염 용액 추출 다당류의 특성 /
[ 조수묵;이재훈;한상배;김환묵;유승헌;유익동 ] / 한국균학회지
8 Fomitella fraxinea로부터 분리한 면역활성 다당류(II)- 열수추출 다당류의 분리 및 특성 /
[ 조수묵;이재훈;한상배;김환묵;유승헌;유익동 ] / 한국균학회지
9 잣버섯 성분의 면역세포 및 전사인자의 활성화 작용에 관한 연구 /
[ 진미림 ] / 서울대학교 대학원 논문집
10 영지의 균사체성 <TEX>${\beta}-glucan$</TEX>에 의한 Raw 264.7 대식세포의 Nitric Oxide 생성 /
[ 한만덕;이은숙;김영권;이중우;정훈;윤경하 ] / 한국균학회지
11 Effect of various extrinsic and intrinsic factors on the nitric oxide production of murine macrophage /
[ Choi, S,H.;Jun, C.D.;Lee, B.S.;Park, S.D.;Oh, J.S.;Chung, H.T. ] / Kor. J. BRM
12 Studies on constituents of higher fungi of Korea (LXVII), antitumor componets of the basidiocarp of Ganoderma-lucidum /
[ Hyun, J.W.;Choi, E.C.;Kim B.K. ] / Kor. J. Mycol.
13 <TEX>$INF-{\gamma}$</TEX> induced 1-arginine-dependent toxoplasmastic activity in murine peritoneal macrophages is mediated by endogenous tumor necrosis <TEX>$factor-{\alpha}$</TEX> /
[ Jan, A.M.;Marielle, E.B.;Peter, H.N.;Pieter, S.H.;Ralph, V.F. ] / J. lmmunol.
14 Mycelial culture and antineoplastic component of Ganoderma lucidum /
[ Kang, C.Y.;Shim, M.J.;Choi, E.C.;Lee, Y.N.;Kim, B.K. ] / Kor. Biochem. J.
15 Studies on the antineoplastic components Korean Basidiomycetes /
[ Kim, B.K.;Chung, H.S.;Chung, K.S.;Yang, M.S. ] / Kor. J. Mycol.
16 Studies on constituents of higher fugni of Korea (XXIV), antineoplastic activities of Coriolus vesicolor(Fr.) Quel, Pleurotus ostreatus (Fr.) Kummer and Lentinus edodes (Berk.) Sing /
[ Kim, B.K.;Park, E.K.;Shim, M.J. ] / Arch. Pharm. Res.   DOI
17 Immunological studies on the antitumor component of Lyophy/lum decastes (II) /
[ Lee, C.O.;Choi, E.C.;Kim, B.K. ] / J. Kor. Cancer Assoc.
18 In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts /
[ Mossman, B.T. ] / Environ. Health Perspect.   DOI
19 Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens /
[ Ohno, N.;Arai, Y.;Suzuki, I.;Yadomae, T. ] / J. Phamacobio-Dyn.   DOI
20 Role of macrophages in the immunotherapy of lewis lung peritoneal carcinomatosisis /
[ Wallace, P.K.;Morahan, P.S. ] / J. Leukoc. Biol.
21 Studies on inorganic composition and immunopotentiating activity of Ganoderma lucidum in Korea (XLVI) /
[ Shin, H.W.;Kim, H.W.;Choi, E.C.;Toh, S.H.;Kim, B.K. ] / Kor. J, Pharmacogn.
22 Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability /
[ Denizot, F.;Lang, R. ] / J. Immunol. Methods.   DOI   ScienceOn
23 밀버섯의 항암성분에 관한 연구 /
[ 김숙희;김진숙;진미림;김하원;치웅칠;김병각 ] / 생약학회지