Browse > Article
http://dx.doi.org/10.4489/KJM.2002.30.2.078

Analysis of Genetic Relationship and Cultural Characteristics of Penicillium species Isolated from Postharvest Decay of Pear by Random Amplified Polymorphic DNA  

Kim, Ju-Hee (Jeollabuk-do Agricultural Research and Extension Services)
Lee, Wang-Hyu (Faculty Biological Resources Science, Chonbuk National University)
Ryu, Young-Jin (Jeollabuk-do Agricultural Research and Extension Services)
Cheong, Seong-Soo (Jeollabuk-do Agricultural Research and Extension Services)
Choi, Joung-Sik (Jeollabuk-do Agricultural Research and Extension Services)
Publication Information
The Korean Journal of Mycology / v.30, no.2, 2002 , pp. 78-85 More about this Journal
Abstract
Fifteen isolates from pear, and sixteen isolates from KCTC, KCCM, and Chungnam Univ. of Penicillium spp. were investigated for the analysis of their relationships of cultural characteristics and RAPD genetic variation by RAPD. The cultural characteristics of Penicillium spp. were shown different growth rate, morphology, and color. In addition, the cultural characteristics and RAPD analysis were conducted for the pear rot pathogens and related isolates. RAPD patterns were applied to compare the taxonomic and genetic diversity of the Penicillium species between 15 groups isolated from pear fruits and 16 standard species. The genomic DNA were amplified from $0.1{\sim}2.0kb$ by five URP primer and 744 bands were detected. The cluster analysis showed four genomic DNA RAPD groups and its similarity was 47.7%. Intraspecific relationships were 87.4, 97.5 and 95.2%, in P. expansum, P. solitum, and P. crustosum, respectively. These results appeared to be that there were high similarities between isolates, and consistent with the results of cultural morphological characteristics analysis.
Keywords
Genetic relationship; Pear; P. crustosum; P. expansum; P. solitum; RAPD;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jo. W. S., Rew, Y. H., Kim. S. H., Yeun, J. T. and Choi, B. S. 1999. Occurrence of Bluish Green Mold of Pleurotus eryngii by Penicillium corylophilum. Korean J. Mycol. 27: 412-414.   과학기술학회마을
2 Kim, R. Y., Kang, H. W., Yu, S. H., Itoh, Y. and Kohmoto, K. 1998. RAPD analysis of host-specific toxin (HST) producing Alternaria species. Kor. J. Plant Pathol. 14: 92-98.   과학기술학회마을
3 Kob, Y. J., Seo, J. K., Lee, T. S., Song, J. H., Kwon, H. M., Moon, D. Y., Moon. D. K. and Han, H. R. 1998. Genetic diversity of Phomopsis citri with random amplified polymorphic DNA (RAPD) and fungicide resistance. Kor. J. Plant Pathol. 14: 171-176.   과학기술학회마을
4 Okuda, T. 1994. Variation in colony characteristics of Penicillium strains resulting from minor variation culture conditions. Mycologia 86: 259-262.   DOI
5 Sequerra, J., Marmeisse, R., Valla, G., Normand, P., Capellano, A. and Moiround, A. 1997. Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribosomal intergenic spacer and random amplified polymorphic DNA. Mycol. Res. 101: 465-472.   DOI   ScienceOn
6 Kelly, A., Alcala-Jimenes, A. R., Bainbridge, B. W., Heale, J. R., Perez-Artes, E. and Jimenez-Diaz, R. M. 1994. Use of genetic fingerprinting and random amplified polymorphic DNA to characterize pathotypes of Fusarium oxysporum f. sp. ciceris infecting chickpea. Phytopathology 84: 1293-1298.   DOI
7 Boysen, M., Skouboe. P., Frisvad, J. and Rossen, L. 1996. Reclassification of the Penicillium roqeuforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiology 142: 541-549.   DOI   ScienceOn
8 Brand, M. E., Padhye, A. A., Mayer, L. W. and Holloway, B. P. 1998. Utility of random amplified polymorphic DNA PCR and taqman automated detection in molecular identification of Aspergillus fumigatus. J. Clinical Microbiol. 36: 2057-2062.
9 Cho, W. D., Kim, W. G., and Kim, H. M. 1995. Fungi associated with storage diseases of garlic. RDA. J. Agri. Sci. 37. 325-329.
10 Hocking, A. D., Whitelaw, M. and Harden. T. J. 1998. Penicillium radicum sp. nov. from the rhizosphere of Australian wheat. Mycol. Res. 102: 801-806.   DOI   ScienceOn
11 Holmes, G. J., Eckert, J. W. and Pitt, J. I. 1994. Revised description of Penicillium ulaiense and its role as a pathogen of citrus fruits. Phytopathology 84: 719-727.   DOI
12 Hyun, J. W. and Park, W. M. 1996. Differentiation of Fusarium oxysporum f. sp. fragariae Isolates by Random amplified Polymorphic DNA (RAPD) Analysis. Korean J. Plant. Pathol. 12: 41-46.   과학기술학회마을
13 Hong, S. Y., Kim, W. G., Cho, W. D. and Lee, Y. H. 1991. Fungi associated with storage disease of citrus fruits. Res. Rept. RDA (C. P.)33: 12-17.