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http://dx.doi.org/10.4489/KJM.2002.30.2.113

Purification and Characterization of Carboxymethyl Cellulase from Stropharia rugosoannulata  

Yoo, Kwan-Hee (Department of Life Science Sang-Ji University)
Chang, Hyung-Soo (Department of Food & Nutrition, Sang-Ji University)
Publication Information
The Korean Journal of Mycology / v.30, no.2, 2002 , pp. 113-118 More about this Journal
Abstract
A Carboxymethyl Cellulase (CMCase) has been isolated and purified from the edible mushroom, Stropharia rugosoannulata. The molecular weight of CMCase was estimated to be 54 kDa by SDS polyacryl amide gel electrophoresis. The maximum activity of the purified CMCase was observed at pH 4.0 and $40^{\circ}C$, and stable for pH 3.0 to 11.0 to maintain 40% activity. The CMCase activity was activated by $AgNO_{3},\;MgSO_{4},\;and\;KCl$. However, its activity was inhibited by 1,10-phenanthroline, KCN and L-cysteine. Also, the enzyme activity was decreased by the addition of EDTA, suggesting that the purified CMCase is metalloenzyme.
Keywords
Carboxymethyl Cellulase; Metalloenzyme; Stropharia rugosoannulata;
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