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http://dx.doi.org/10.4489/KJM.2002.30.1.066

Development of Repetitive DNA Probes for Genetic Analysis of Phytophthora capsici  

Song, Jeong-Young (Department of Agricultural Biology, Chungnam National University)
Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
Publication Information
The Korean Journal of Mycology / v.30, no.1, 2002 , pp. 66-72 More about this Journal
Abstract
To develop DNA markers for analysis of genetic characteristics of Phytophthora capsici population, randomly selected clones from HindIII-digested genomic DNA library of P. capsici 95CY3119 were surveyed by hybridizing to Southern blots of HindIII-digested total genomic DNA of P. capsici. Probe DNAs inserted into selected individual clones strongly hybridized with HindIII digests of P. capsici. Among probes examined, PC9 revealed the repetitive and highly polymorphic bands to HindIII digests of inter-and intra-field P. capsici isolates. Genetic diversity of individual isolates was also clearly revealed in cluster analysis based on its band patterns. The other probe, PC22, was hybridized only to DNA from P. capsici and this was highly repetitive. However, there was no response to other Phytophthora species and Pythium sp. These DNA probes could be used as very useful markers in analysing genetic diversity and identification for P. capsici population throughout the world.
Keywords
Genetic analysis; Pepper; Phytophthora blight; Phytophthora capsici; Species-specific probe;
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