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http://dx.doi.org/10.3740/MRSK.2007.17.12.669

The Effects of HAp and BCP Nano Powders Synthesized by Microwave-Assisted Synthesis on the Activation of Osteoblast and Osteoclast  

Song, Ho-Yeon (Department of Microbiology School of Medicine, Soonchunhyang University)
Min, Young-Ki (Department of Physiology, School of Medicine, Soonchunhyang University)
Yang, Hun-Mo (Department of Physiology, School of Medicine, Soonchunhyang University)
Mang, Joo-Yang (Department of Chemistry Soonchunhyang University)
Publication Information
Korean Journal of Materials Research / v.17, no.12, 2007 , pp. 669-675 More about this Journal
Abstract
Hydroxyapatite (HAp) and biphasic calcium phosphate (BCP) nano powders were synthesized using the microwave-assisted synthesis process dependent on pH and microwave irradiation time. The average size of a powder was less than 100 nm in diameter. Through in-vitro cytotoxicity tests by an extract dilution method, the HAp and BCP nano powders have shown to be cytocompatible for L-929 fibroblast cells, osteoblastlike MG-63 cells and osteoclast-like Raw 264.7 cells. The activation of osteoblast was estimated by alkaline phosphatase (ALP) activity. When the HAp and BCP were treated to MG-63 cells, alkaline phosphatase activities increased on day 3, compared with those of the untreated cells. Also, the collagen fibers increased when the HAp and BCP powders suspension were treated to MG-63 cells, compared to those of the untreated cells. Quantitative alizarin red S mineralization assays showed a trend toward increasing mineralization in osteoblast cultured with powder suspension. In conclusion, hydroxyapatite and biphasic calcium phosphate appeared to be a bone graft substitute material with optimal biocompatibility and could be further applied to clinical use as an artificial bone graft substitute.
Keywords
Hydroxyapatite; Biphasic calcium phosphate; Microwave; Biocompatibility;
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Times Cited By SCOPUS : 2
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