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http://dx.doi.org/10.3740/MRSK.2006.16.10.606

Influence of Anodic Oxidation Film Formed on Titanium onto Cell Attachment and Proliferation  

Noh, Se-Ra (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University)
Lee, Yong-Ryeol (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University)
Song, Ho-Jun (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University)
Park, Yeong-Joon (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University)
Publication Information
Korean Journal of Materials Research / v.16, no.10, 2006 , pp. 606-613 More about this Journal
Abstract
This study was purposed to evaluate the influence of anodically oxidized film on titanium (Ti) onto MG-63 osteoblast-like cell attachment and activity. Only scratch lines created by polishing were seen in ASR and ANO-1 groups. About $1.5{\mu}m$-thick homogeneous oxide film which has pores of about $0.5{\mu}m$ diameter were formed in ANO-12. The crystalline structure of the oxide films formed by anodization in phosphoric acid electrolyte was $TiP_2O_7$. The total protein amounts of ANO-1 and ANO-12 groups showed higher values of maximum protein amount than that of AS-R group. At 3 days of incubation, total protein amount showed higher value in ANO-2 when comparing to that of AS-R (p<0.05). Based on the results of ALPase activity test, the degree of MG-63 cell differentiation for initial mineralization matrix formation was similar. For all the test groups after 1 day of incubation, MG-63 cells grew healthily in mono-layer with dendritic extensions. After incubation for 3 days, the specimen surfaces were covered more densely by cells, and numerous micro filaments were extruding to the extracellular matrix.
Keywords
anodization; titanium oxide; implant; osteoblast-like cell; ALPase activity;
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