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http://dx.doi.org/10.17703/JCCT.2021.7.3.605

Establishment of Efficient Callus Induction System with Picloram Hormone in Lily Plants  

Kim, Jong Bo (Dept. of Biotechnology, Konkuk Univ)
Publication Information
The Journal of the Convergence on Culture Technology / v.7, no.3, 2021 , pp. 605-608 More about this Journal
Abstract
Lily is one of the most important 5 cut flowers in international flower market and lilies are distributed in Asia, Eurasia and North America. To develop a new lily cultivar, in addition to hybridization, mutation and selection methods, biotechnological techniques including tissue culture are also required. Establishment of tissue culture system is one of the requirement for the breeding program in Lily. Among many fields of plant tissue culture, establishment of regeneration system via embryogenic calluses are studied in many crops. In this study, research was carried out to decide the proper concentration of picloram which is used for the induction of embryogenic calluses. As a result, 3 different types of callused were observed after 3-4 weeks. They were CEC (compact embryogenic callus), FEC (friable embryogenic callus) and white callus type. 1.0 mg /l of picloram showed the best result for the production of embryogenic callus, however, due to its higher rate of browning in this concentration, 0.75 mg/l of picloram was selected as a proper concentration of picloram for the induction of CEC and FEC in Lily. These results can be contributed to the establishment of both regeneration system and mass propagation in lily in the future.
Keywords
Callus; Lily; Mass Propagation; Regeneration; Picloram;
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