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http://dx.doi.org/10.4046/trd.2014.76.3.120

Apigenin and Wogonin Regulate Epidermal Growth Factor Receptor Signaling Pathway Involved in MUC5AC Mucin Gene Expression and Production from Cultured Airway Epithelial Cells  

Sikder, Md. Asaduzzaman (Department of Pharmacology, Chungnam National University School of Medicine)
Lee, Hyun Jae (Department of Pharmacology, Chungnam National University School of Medicine)
Ryu, Jiho (Department of Pharmacology, Chungnam National University School of Medicine)
Park, Su Hyun (Department of Pharmacology, Chungnam National University School of Medicine)
Kim, Ju-Ock (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine)
Hong, Jang-Hee (Department of Pharmacology, Chungnam National University School of Medicine)
Seok, Jeong Ho (Department of Pharmacology, Chungnam National University School of Medicine)
Lee, Choong Jae (Department of Pharmacology, Chungnam National University School of Medicine)
Publication Information
Tuberculosis and Respiratory Diseases / v.76, no.3, 2014 , pp. 120-126 More about this Journal
Abstract
Background: We investigated whether wogonin and apigenin significantly affect the epidermal growth factor receptor (EGFR) signaling pathway involved in MUC5AC mucin gene expression, and production from cultured airway epithelial cells; this was based on our previous report that apigenin and wogonin suppressed MUC5AC mucin gene expression and production from human airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with wogonin or apigenin for 15 minutes or 24 hours and then stimulated with epidermal growth factor (EGF) for 24 hours or the indicated periods. Results: We found that incubation of NCI-H292 cells with wogonin or apigenin inhibited the phosphorylation of EGFR. The downstream signals of EGFR such as phosphorylation of MEK1/2 and ERK1/2 were also inhibited by wogonin or apigenin. Conclusion: The results suggest that wogonin and apigenin inhibits EGFR signaling pathway, which may explain how they inhibit MUC5AC mucin gene expression and production induced by EGF.
Keywords
Receptor; Epidermal Growth Factor; Mucins; Wogonin; Apigenin;
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1 Zhou Y, Rajabi H, Kufe D. Mucin 1 C-terminal subunit oncoprotein is a target for small-molecule inhibitors. Mol Pharmacol 2011;79:886-93.   DOI   ScienceOn
2 Li RR, Pang LL, Du Q, Shi Y, Dai WJ, Yin KS. Apigenin inhibits allergen-induced airway inflammation and switches immune response in a murine model of asthma. Immunopharmacol Immunotoxicol 2010;32:364-70.   DOI   ScienceOn
3 Cho J, Lee HK. Wogonin inhibits ischemic brain injury in a rat model of permanent middle cerebral artery occlusion. Biol Pharm Bull 2004;27:1561-4.   DOI   ScienceOn
4 Chun W, Lee HJ, Kong PJ, Lee GH, Cheong IY, Park H, et al. Synthetic wogonin derivatives suppress lipopolysaccharideinduced nitric oxide production and hydrogen peroxideinduced cytotoxicity. Arch Pharm Res 2005;28:216-9.   DOI
5 Sikder MA, Lee HJ, Mia MZ, Park SH, Ryu J, Kim JH, et al. Inhibition of TNF-alpha-induced MUC5AC mucin gene expression and production by wogonin through the inactivation of NF-kappaB signaling in airway epithelial cells. Phytother Res 2014;28:62-8.   DOI   ScienceOn
6 Kim JO, Sikder MA, Lee HJ, Rahman M, Kim JH, Chang GT, et al. Phorbol ester or epidermal growth-factor-induced MUC5AC mucin gene expression and production from airway epithelial cells are inhibited by apigenin and wogonin. Phytother Res 2012;26:1784-8.   DOI   ScienceOn
7 Burgel PR, Nadel JA. Roles of epidermal growth factor receptor activation in epithelial cell repair and mucin production in airway epithelium. Thorax 2004;59:992-6.   DOI   ScienceOn
8 Sampson L, Rimm E, Hollman PC, de Vries JH, Katan MB. Flavonol and flavone intakes in US health professionals. J Am Diet Assoc 2002;102:1414-20.   DOI   ScienceOn
9 Lee HJ, Lee SY, Bae HS, Kim JH, Chang GT, Seok JH, et al. Inhibition of airway MUC5AC mucin production and gene expression induced by epidermal growth factor or phorbol ester by glycyrrhizin and carbenoxolone. Phytomedicine 2011;18:743-7.   DOI   ScienceOn
10 Lee HJ, Lee SY, Jeon BK, Lee JW, Kim YS, Lee MN, et al. Effect of platycodin D on airway MUC5AC mucin production and gene expression induced by growth factor and proinflammatory factor. Biomol Ther 2010;18:294-9.   DOI   ScienceOn
11 Lee HJ, Lee SY, Lee MN, Kim JH, Chang GT, Seok JH, et al. Inhibition of secretion, production and gene expression of mucin from cultured airway epithelial cells by prunetin. Phytother Res 2011;25:1196-200.   DOI   ScienceOn
12 Heo HJ, Lee SY, Lee MN, Lee HJ, Seok JH, Lee CJ. Genistein and curcumin suppress epidermal growth factor-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Phytother Res 2009;23:1458-61.   DOI   ScienceOn
13 Jang IM. Treatise on Asian herbal medicines. Seoul: Haksul- Pyunsukwan in Research Institute of Natural Products of Seoul National University; 2003.
14 Rogers DF, Barnes PJ. Treatment of airway mucus hypersecretion. Ann Med 2006;38:116-25.   DOI   ScienceOn
15 Clere N, Faure S, Martinez MC, Andriantsitohaina R. Anticancer properties of flavonoids: roles in various stages of carcinogenesis. Cardiovasc Hematol Agents Med Chem 2011;9:62-77.   DOI
16 Paoletti T, Fallarini S, Gugliesi F, Minassi A, Appendino G, Lombardi G. Anti-inflammatory and vascularprotective properties of 8-prenylapigenin. Eur J Pharmacol 2009;620:120-30.   DOI   ScienceOn
17 Basbaum C, Lemjabbar H, Longphre M, Li D, Gensch E, Mc- Namara N. Control of mucin transcription by diverse injuryinduced signaling pathways. Am J Respir Crit Care Med 1999;160(5 Pt 2):S44-8.   DOI
18 Kohri K, Ueki IF, Shim JJ, Burgel PR, Oh YM, Tam DC, et al. Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor. Eur Respir J 2002;20:1263-70.   DOI   ScienceOn
19 Mata M, Sarria B, Buenestado A, Cortijo J, Cerda M, Morcillo EJ. Phosphodiesterase 4 inhibition decreases MUC5AC expression induced by epidermal growth factor in human airway epithelial cells. Thorax 2005;60:144-52.   DOI   ScienceOn
20 Wetzker R, Bohmer FD. Transactivation joins multiple tracks to the ERK/MAPK cascade. Nat Rev Mol Cell Biol 2003;4:651-7.   DOI   ScienceOn
21 Li JD, Dohrman AF, Gallup M, Miyata S, Gum JR, Kim YS, et al. Transcriptional activation of mucin by Pseudomonas aeruginosa lipopolysaccharide in the pathogenesis of cystic fibrosis lung disease. Proc Natl Acad Sci U S A 1997;94:967-72.   DOI   ScienceOn
22 Takeyama K, Dabbagh K, Lee HM, Agusti C, Lausier JA, Ueki IF, et al. Epidermal growth factor system regulates mucin production in airways. Proc Natl Acad Sci U S A 1999;96:3081-6.   DOI   ScienceOn
23 Lemmon MA, Schlessinger J. Regulation of signal transduction and signal diversity by receptor oligomerization. Trends Biochem Sci 1994;19:459-63.   DOI   ScienceOn
24 Yamauchi T, Ueki K, Tobe K, Tamemoto H, Sekine N, Wada M, et al. Tyrosine phosphorylation of the EGF receptor by the kinase Jak2 is induced by growth hormone. Nature 1997;390:91-6.   DOI   ScienceOn
25 Hunter T, Cooper JA. Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells. Cell 1981;24:741-52.   DOI   ScienceOn
26 Goldkorn T, Balaban N, Matsukuma K, Chea V, Gould R, Last J, et al. EGF-Receptor phosphorylation and signaling are targeted by H2O2 redox stress. Am J Respir Cell Mol Biol 1998;19:786-98.   DOI   ScienceOn
27 Rosette C, Karin M. Ultraviolet light and osmotic stress: activation of the JNK cascade through multiple growth factor and cytokine receptors. Science 1996;274:1194-7.   DOI   ScienceOn
28 Greenberg AK, Basu S, Hu J, Yie TA, Tchou-Wong KM, Rom WN, et al. Selective p38 activation in human non-small cell lung cancer. Am J Respir Cell Mol Biol 2002;26:558-64.   DOI   ScienceOn
29 Zhong Y, Krisanapun C, Lee SH, Nualsanit T, Sams C, Peungvicha P, et al. Molecular targets of apigenin in colorectal cancer cells: involvement of p21, NAG-1 and p53. Eur J Cancer 2010;46:3365-74.   DOI   ScienceOn
30 Amishima M, Munakata M, Nasuhara Y, Sato A, Takahashi T, Homma Y, et al. Expression of epidermal growth factor and epidermal growth factor receptor immunoreactivity in the asthmatic human airway. Am J Respir Crit Care Med 1998;157(6 Pt 1):1907-12.   DOI   ScienceOn
31 Shao MX, Ueki IF, Nadel JA. Tumor necrosis factor alphaconverting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells. Proc Natl Acad Sci U S A 2003;100:11618-23.   DOI   ScienceOn