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Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats  

Moon, Yongsuck (Department of Anatomy, Catholic University of Daegu, School of Medicine)
Kim, Jihye (Department of Physiology, Catholic University of Daegu, School of Medicine)
Lee, Young Man (Department of Physiology, Catholic University of Daegu, School of Medicine)
Publication Information
Tuberculosis and Respiratory Diseases / v.65, no.6, 2008 , pp. 464-470 More about this Journal
Abstract
Background: This study examined the role of neutrophilc oxidative stress in an $O_2-induced$ acute lung injury (ALI). Methods: For 48 h, experimental rats were exposed to pure oxygen (normobaric hyperoxia) in a plastic cage. Forty-eight hours after $O_2$ breathing, the rats were sacrificed and the parameters for ALI associated with neutrophilic oxidative stress were assessed Results: Normobaric pure oxygen induced ALI, which was quite similar to ARDS. The $O_2-induced$ neutrophilic oxidative stress was identified by confirming of the increase in lung myeloperoxidase, BAL neutrophils, malondialdehyde (MDA), cytosolic phospholipase $A_2$ ($cPLA_2$) activity in the lung, histological changes and BAL cytospin morphology. Conclusion: In part, ALI-caused by oxygen is affected by neutrophils especially by the generation of free radicals.
Keywords
Acute lung injury; $O_2$ toxicity; Neutrophils;
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