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Detection of Clarithromycin-resistant Strains from Clinical Isolates of Mycobacterium abscessus  

Lee, Seung Heon (Korean Institute of Tuberculosis)
Park, Young Kil (Korean Institute of Tuberculosis)
Ryo, Sung Weon (Korean Institute of Tuberculosis)
Shim, Myung Sup (Korean Institute of Tuberculosis)
Lew, Woo Jin (Korean Institute of Tuberculosis)
Kim, Hee Jin (Korean Institute of Tuberculosis)
Publication Information
Tuberculosis and Respiratory Diseases / v.64, no.6, 2008 , pp. 422-426 More about this Journal
Abstract
Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.
Keywords
Mycobacterium abscessus; Reverse hybridization assay; Broth microdilution test; 23S rRNA; Clarithromycin;
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