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IP-10 Decreases TNF-α Induced MUC5AC Expression in Human Airway Epithelial Cells: a Possible Relation with Little Sputum Production in Idiopathic Pulmonary Fibrosis  

Kim, Seung Joon (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Kang, Chun Mi (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
You, Moon Bin (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Yoon, Hyung Kyu (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Kim, Young Kyoon (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Kim, Kwan Hyoung (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Moon, Hwa Sik (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Park, Sung Hak (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Song, Jeong Sup (Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Publication Information
Tuberculosis and Respiratory Diseases / v.64, no.5, 2008 , pp. 347-355 More about this Journal
Abstract
Background: IPF is characterized by chronic, fibrosing inflammatory lung disease of unknown etiology. Typical symptoms of IPF are exertional dyspnea with nonproductive cough. Why patients with typical IPF have dry cough rather than productive cough, is unknown. IP-10 plays an important regulatory role in leukocyte trafficking into the lung. The present study investigated the effect of IP-10 in the pathogenesis of dry cough rather than productive cough in IPF patients. Methods: IP-10 concentration was measured by ELISA from BALF of IPF patients. To evaluate the role of IP-10 in mucin expression, the expression of the MUC5AC mucin gene was measured in NCI-H292 cells, a human pulmonary mucoepidermoid carcinoma cell line, after stimulation by TNF-${\alpha}$ with or without IP-10 pretreatment. EGFR-MAPK expression was also examined as a possible mechanism. Results: IP-10 levels were significantly higher in the BALF of IPF patients compared to healthy controls. IP-10 pretreatment reduced TNF-${\alpha}$ induced MUC5AC mucin expression by inhibiting the EGFR-MAPK signal transduction pathway in NCI-H292 cells. Conclusion: These findings suggest that little mucus production in IPF patients might be attributable to IP-10 overproduction, which inhibits the EGFR-MAPK signal transduction pathway required for MUC5AC mucin gene expression.
Keywords
Idiopathic pulmonary fibrosis; MUC5AC; IP-10; Epidermal growth factor receptor; Mitogen-activated protein kinase;
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1 Brown KK. Chronic cough due to chronic interstitial pulmonary diseases: ACCP evidence-based clinical practice guidelines. Chest 2006;129:180S-5S   DOI   ScienceOn
2 Vinall LE, Fowler JC, Jones AL, Kirkbride HJ, de Bolos C, Laine A, et al. Polymorphism of human mucin genes in chest disease: possible significance of MUC2. Am J Respir Cell Mol Biol 2000;23:678-86   DOI   ScienceOn
3 Song JS, Kang CM, Yoo MB, Kim SJ, Yoon HK, Kim YK, et al. Nitric oxide induces MUC5AC mucin in respiratory epithelial cells through PKC and ERK dependent pathways. Respir Res 2007;8:28   DOI   ScienceOn
4 Louahed J, Toda M, Jen J, Hamid Q, Renauld JC, Levitt RC, et al. Interleukin-9 upregulates mucus expression in the airways. Am J Respir Cell Mol Biol 2000;22:649- 56   DOI   ScienceOn
5 Song KS, Lee WJ, Chung KC, Koo JS, Yang EJ, Choi JY, et al. Interleukin-1 beta and tumor necrosis factor- alpha induce MUC5AC overexpression through a mechanism involving ERK/p38 mitogen-activated protein kinases-MSK1-CREB activation in human airway epithelial cells. J Biol Chem 2003;278:23243-50   DOI   ScienceOn
6 Luster AD, Ravetch JV. Biochemical characterization of a gamma interferon-inducible cytokine (IP-10). J Exp Med 1987;166:1084-97   DOI   ScienceOn
7 Pignatti P, Brunetti G, Moretto D, Yacoub MR, Fiori M, Balbi B, et al. Role of the chemokine receptors CXCR3 and CCR4 in human pulmonary fibrosis. Am J Respir Crit Care Med 2006;173:310-7   DOI   ScienceOn
8 Taub DD, Lloyd AR, Conlon K, Wang JM, Ortaldo JR, Harada A, et al. Recombinant human interferon-inducible protein 10 is a chemoattractant for human monocytes and T lymphocytes and promotes T cell adhesion to endothelial cells. J Exp Med 1993;177:1809- 14   DOI
9 Liu L, Callahan MK, Huang D, Ransohoff RM. Chemokine receptor CXCR3: an unexpected enigma. Curr Top Dev Biol 2005;68:149-81   DOI   ScienceOn
10 Medoff BD, Sauty A, Tager AM, Maclean JA, Smith RN, Mathew A, et al. IFN-gamma-inducible protein 10 (CXCL10) contributes to airway hyperreactivity and airway inflammation in a mouse model of asthma. J Immunol 2002;168:5278-86   DOI
11 Keane MP, Arenberg DA, Lynch JP 3rd, Whyte RI, Iannettoni MD, Burdick MD, et al. The CXC chemokines, IL-8 and IP-10, regulate angiogenic activity in idiopathic pulmonary fibrosis. J Immunol 1997;159: 1437-43
12 Shiraha H, Glading A, Gupta K, Wells A. IP-10 inhibits epidermal growth factor-induced motility by decreasing epidermal growth factor receptor-mediated calpain activity. J Cell Biol 1999;146:243-54
13 Takeyama K, Dabbagh K, Jeong Shim J, Dao-Pick T, Ueki IF, Nadel JA. Oxidative stress causes mucin synthesis via transactivation of epidermal growth factor receptor: role of neutrophils. J Immunol 2000;164:1546- 52   DOI
14 Chorley BN, Crews AL, Li Y, Adler KB, Minnicozzi M, Martin LD. Differential Muc2 and Muc5ac secretion by stimulated guinea pig tracheal epithelial cells in vitro. Respir Res 2006;7:35   DOI   ScienceOn
15 Sauty A, Dziejman M, Taha RA, Iarossi AS, Neote K, Garcia-Zepeda EA, et al. The T cell-specific CXC chemokines IP-10, Mig, and I-TAC are expressed by activated human bronchial epithelial cells. J Immunol 1999;162:3549-58
16 Saetta M, Mariani M, Panina-Bordignon P, Turato G, Buonsanti C, Baraldo S, et al. Increased expression of the chemokine receptor CXCR3 and its ligand CXCL10 in peripheral airways of smokers with chronic obstructive pulmonary disease. Am J Respir Crit Care Med 2002;165:1404-9   DOI   ScienceOn
17 Voynow JA, Selby DM, Rose MC. Mucin gene expression (MUC1, MUC2, and MUC5/5AC) in nasal epithelial cells of cystic fibrosis, allergic rhinitis, and normal individuals. Lung 1998;176:345-54   DOI
18 Liebmann C. Regulation of MAP kinase activity by peptide receptor signalling pathway: paradigms of multiplicity. Cell Signal 2001;13:777-85   DOI   ScienceOn
19 Song JS, Cho KS, Yoon HK, Moon HS, Park SH. Neutrophil elastase causes MUC5AC mucin synthesis via EGF receptor, ERK and NF-kB pathways in A549 cells. Korean J Intern Med 2005;20:275-83   DOI   ScienceOn
20 Bogdan S, Klambt C. Epidermal growth factor receptor signaling. Curr Biol 2001;11:R292-5   DOI   ScienceOn
21 Johnston ID, Prescott RJ, Chalmers JC, Rudd RM. British Thoracic Society study of cryptogenic fibrosing alveolitis: current presentation and initial management. Fibrosing Alveolitis Subcommittee of the Research Committee of the British Thoracic Society. Thorax 1997;52:38-44   DOI   ScienceOn
22 Levine SJ, Larivee P, Logun C, Angus CW, Ognibene FP, Shelhamer JH. Tumor necrosis factor-alpha induces mucin hypersecretion and MUC-2 gene expression by human airway epithelial cells. Am J Respir Cell Mol Biol 1995;12:196-204   DOI   ScienceOn
23 Agostini C, Cassatella M, Zambello R, Trentin L, Gasperini S, Perin A, et al. Involvement of the IP-10 chemokine in sarcoid granulomatous reactions. J Immunol 1998;161:6413-20
24 Baughman RP, Lower EE, Miller MA, Bejarano PA, Heffelfinger SC. Overexpression of transforming growth factor-alpha and epidermal growth factor-receptor in idiopathic pulmonary fibrosis. Sarcoidosis Vasc Diffuse Lung Dis 1999;16:57-61
25 Song KS, Seong JK, Chung KC, Lee WJ, Kim CH, Cho KN, et al. Induction of MUC8 gene expression by interleukin- 1 beta is mediated by a sequential ERK MAPK/ RSK1/CREB cascade pathway in human airway epithelial cells. J Biol Chem 2003;278:34890-6   DOI   ScienceOn
26 Miotto D, Christodoulopoulos P, Olivenstein R, Taha R, Cameron L, Tsicopoulos A, et al. Expression of IFNgamma- inducible protein; monocyte chemotactic proteins 1, 3, and 4; and eotaxin in TH1- and TH2-mediated lung diseases. J Allergy Clin Immunol 2001;107:664- 70   DOI   ScienceOn
27 Coultas DB, Zumwalt RE, Black WC, Sobonya RE. The epidemiology of interstitial lung diseases. Am J Respir Crit Care Med 1994;150:967-72   DOI   ScienceOn
28 Chang L, Karin M. Mammalian MAP kinase signalling cascades. Nature 2001;410:37-40   DOI   ScienceOn
29 American Thoracic Society; European Respiratory Society. American Thoracic Society/European Respiratory Society International Multidisciplinary Consensus Classification of the Idiopathic Interstitial Pneumonias. This joint statement of the American Thoracic Society (ATS), and the European Respiratory Society (ERS) was adopted by the ATS board of directors, June 2001 and by the ERS Executive Committee, June 2001. Am J Respir Crit Care Med 2002;165:277-304   DOI   ScienceOn
30 Nakayama S, Mukae H, Ishii H, Kakugawa T, Sugiyama K, Sakamoto N, et al. Comparison of BALF concentrations of ENA-78 and IP10 in patients with idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia. Respir Med 2005;99:1145-51   DOI   ScienceOn
31 Agostini C, Facco M, Siviero M, Carollo D, Galvan S, Cattelan AM, et al. CXC chemokines IP-10 and mig expression and direct migration of pulmonary CD8+/ CXCR3+ T cells in the lungs of patients with HIV infection and T-cell alveolitis. Am J Respir Crit Care Med 2000;162:1466-73   DOI   ScienceOn
32 Takeyama K, Dabbagh K, Lee HM, Agusti C, Lausier JA, Ueki IF, et al. Epidermal growth factor system regulates mucin production in airways. Proc Natl Acad Sci U S A 1999;96:3081-6   DOI