Browse > Article

IL-1Ra Elaboration by Colchicine Stimulation in Normal Human Bronchial Epithelial Cells  

Lee, Jae Hyung (Department of Internal Medicine, Eulji General Hospital, Eulji University School of Medicine)
Kim, Sang Heon (Department of Internal Medicine, College of Medicine, Hanyang University)
Kim, Tae Hyung (Department of Internal Medicine, College of Medicine, Hanyang University)
Sohn, Jang Won (Department of Internal Medicine, College of Medicine, Hanyang University)
Yoon, Ho Joo (Department of Internal Medicine, College of Medicine, Hanyang University)
Shin, Dong Ho (Department of Internal Medicine, College of Medicine, Hanyang University)
Park, Sung Soo (Department of Internal Medicine, College of Medicine, Hanyang University)
Publication Information
Tuberculosis and Respiratory Diseases / v.63, no.2, 2007 , pp. 145-153 More about this Journal
Abstract
Background: Asthma is a syndrome that is characterized by a variable degree of airflow obstruction, bronchial hyperresponsiveness, and airway inflammation. Colchicine is an inexpensive and safe medication with unique anti-inflammatory properties. IL-1Ra (Interleukin-1 receptor antagonist) mediates the anti-inflammatory effect in human inflammatory diseases, including asthma. This study examined whether IL-1Ra mediates the anti-inflammatory effect of colchicine in normal human bronchial epithelial cells (NHBE), RAW 264.7 cells (murine macrophage cell line), and a mouse lung. Methods: NHBE, RAW 264.7 cells and BALB/c mice were stimulated with colchicine, and the increase in the IL-1Ra level was estimated by ELISA, Western analysis and RT-PCR analysis. Results: Colchicine stimulated NHBE and RAW 264.7 cells to release IL-1Ra into the supernatant in a dose-and time-dependent manner. The major isoform of IL-1Ra in NHBE and RAW 264.7 cells is type I icIL-1Ra, and sIL-1Ra, respectively. IL-1Ra up-regulation was blocked by PD98059, a specific inhibitor in MAPK pathways. Colchicine also stimulated the secretion of IL-1Ra into the bronchoalveolar lavage (BAL) fluid of BALB/c mouse. Conclusion: Colchicine stimulates an increase in the IL-1Ra level both in vivo and in vitro, and might have an anti-inflammatory effect.
Keywords
Colchicine; Asthma; IL-1Ra; MAPK;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Haley KJ, Sunday ME, Wiggs BR, Kozakewich HP, Reilly JJ, Mentzer SJ, et al. Inflammatory cell distribution within and along asthmatic airways. Am J Respir Grit Care Med 1998;158:565-72   DOI   ScienceOn
2 Kelly SJ, Uri AJ, Freeland HS, Woods EJ, Schulman ES, Peters SP, et al. Effects of colchicines on IgE-mediated early and late airway reactions. Chest 1995;107:985-91   DOI   ScienceOn
3 Levine SJ, Wu T, Shelhamer JH. Extracellular release of the type I intracellular IL-1 receptor antagonist from human airway epithelial cells: differential effects of IL-4, IL-13, IFN-gamma, and corticosteroids. J Immunol 1997;158:5949-57
4 Okada S, Inoue H, Yamauchi K, Iijima H, Ohkawara Y, Takishima T, et al. Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: suppressive effect of interleukin-1 receptor antagonist on late asthmatic reaction. J Allergy Clin Immunol 1995;95:1236-45   DOI   ScienceOn
5 Busse WW, Lemanske RF Jr. Asthma. N Engl J Med 2001;344:350-62   DOI   ScienceOn
6 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 1987;162:156-9
7 Vignola AM, Chanez P, Campbell AM, Souques F, Lebel B, Enander I, et al. Airway inflammation in mild intermittent and in persistent asthma. Am J Respir Grit Care Med 1998;157:403-9   DOI   ScienceOn
8 Schwarz YA, Kivity S, Ilfeld DN, Schlesinger M, Greif J, Topilsky M, et al. A clinical and immunologic study of colchicines in asthma. J Allergy Clin Immunol 1990;85:578-82   DOI
9 Kraft M, Djukanovic R, Wilson S, Holgate ST, Martin RJ. Alveolar tissue inflammation in asthma. Am J Respir Grit Care Med 1996;154:1505-10   DOI   ScienceOn
10 Selig W, Tacker J. Effect of interleukin-l receptor antagonist on antigen-induced pulmonary responses in guinea pigs. Eur J Pharmacol 1992;213:331-6   DOI   ScienceOn
11 Arend WP, Malyak M, Guthridge CJ, Gabay C. Interleukin-1 receptor antagonist: role in biology. Annu Rev Immunol 1998;16:27-55   DOI   ScienceOn
12 Muzio M, Polentarutti N, Sironi M, Poli G, Th Gioia L, Introna M, et al. Cloning and characterization of a new isoform of the interleukin receptor antagonist. J Exp Med 1995;182:623-8   DOI   ScienceOn
13 Berger AE, Carter DB, Hankey SO, McEwan RN. Cytokine regulation of the interleukin-l receptor antagonist protein in U937 cells. Eur J Immunol 1993;23:39-45   DOI   ScienceOn