Browse > Article

The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury  

Huh, Jin Won (Department of Internal Medicine, Ilsan Paik Hospital, Inje University)
Hong, Sang Bum (Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of ulsan College of Medicine)
Kim, Mi Jung (Asan Institute for Life Sciences)
Lim, Chae-Man (Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of ulsan College of Medicine)
Koh, Younsuck (Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of ulsan College of Medicine)
Publication Information
Tuberculosis and Respiratory Diseases / v.62, no.2, 2007 , pp. 105-112 More about this Journal
Abstract
Background: Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of ${\alpha}$-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI. Materials and Methods: ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+${\alpha}$-lipoic acid, LPS, and LPS+${\alpha}$-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration. Results: The total cell and neutrophil counts of the LPS+${\alpha}$-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+${\alpha}$-lipoic acid groups. The TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 concentrations in the BAL fluid were not decreased by the ${\alpha}$-lipoic acid treatment in the LPS treated rats. Conclusions: Although ${\alpha}$-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study.
Keywords
${\alpha}$-lipoic acid; oxidative stress; acute lung injury; antioxidant;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Weiss SJ. Tissue destruction by neutrophils. N Engl J Med 1989;320:365-76   DOI   ScienceOn
2 Chow CW, Herrera Abreu MT, Suzuki T, Downey GP. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol 2003;29:427-31   DOI   ScienceOn
3 Packer L. alpha-Lipoic acid: a metabolic antioxidant which regulates NF-kappa B signal transduction and protects against oxidative injury. Drug Metab Rev 1998;30:245-75   DOI   ScienceOn
4 Ortolani O, Conti A, De Gaudio AR, Masoni M, Novelli G. Protective effects of N-acetylcysteine and rutin on the lipid peroxidation of the lung epithelium during the adult respiratory distress syndrome. Shock 2000;13:14-8   DOI   ScienceOn
5 Packer L, Witt EH, Tritschler HJ. alpha-Lipoic acid as a biological antioxidant. Free Radic Biol Med 1995;19:227-50   DOI   ScienceOn
6 Moini H, Packer L, Saris NE. Antioxidant and prooxidant activities of alpha-lipoic acid and dihydrolipoic acid. Toxicol Appl Pharmacol 2002;182:84-90   DOI
7 Nitto T, Araki Y, Takeda Y, Sendo F. Pharmacological analysis for mechanisms of GPI-80 release from tumour necrosis factor-alpha-stimulated human neutrophils. Br J Pharmacol 2002;137:353-60   DOI   ScienceOn
8 Antonicelli F, Brown D,Parmentier M, Drost EM, Hirani N, Rahman I, et al. Regulation of LPS-mediated inflammation in vivo and in vitro by the thiol antioxidant Nacystelyn. Am J Physiol Lung Cell Mol Physiol 2004;286:L1319-27   DOI
9 Koh Y, Lee YM, Lim CM, Lee SS, Shim TS, Lee SD, et al. Effects of heat pretreatment on histopathology, cytokine production, and surfactant in endotoxininduced acute lung injury. Inflammation 2001;25:1 87-96   DOI   ScienceOn
10 Islam KN, Devaraj S, Jialal I. alpha-Tocopherol enrichment of monocytes decreases agonist-induced adhesion to human endothelial cells. Circulation 1998;98:2255-61   DOI   ScienceOn
11 Zaluska WT, Ksiazek A, Roliski J. Effect of vitamin E modified cellulose membrane on human lymphocyte, monocyte, and granulocyte CD11b/CD18 adhesion molecule expression during hemodialysis. ASAIO J 2001;47:619-22   DOI   ScienceOn
12 Suntres ZE. Prophylaxis against lipopolysaccharideinduced liver injuries by lipoic acid in rats. Pharmacol Res 2003;48:585-91   DOI   ScienceOn
13 Reljanovic M, Reichel G, Rett K, Lobisch M, Schuette K, Moller W, et al. Treatment of diabetic polyneuropathy with the antioxidant thioctic acid (alpha-lipoic acid): a two year multicenter randomized double-blind placebo-controlled trial (ALADIN II). Free Radic Res 1999;31:171-9
14 van Dam PS. Oxidative stress and diabetic neuropathy: pathophysiological mechanisms and treatment perspectives. Diabetes Metab Res Rev 2002;18:176-84   DOI   ScienceOn
15 Bhatti F, Mankhey RW, Asico L, Quinn MT, Welch WJ, Maric C. Mechanisms of antioxidant and pro-oxidant effects of alpha-lipoic acid in the diabetic and nondiabetic kidney. Kidney Int 2005;67:1371-80   DOI   ScienceOn
16 Chignard M, Balloy V. Neutrophil recruitment and increased permeability during acute lung injury induced by lipopolysaccharide. Am J Physiol Lung Cell Mol Physiol 2000;279:L1083-90   DOI
17 Serrano CV Jr, Mikhail EA, Wang P, Noble B, Kuppusamy P, Zweier JL. Superoxide and hydrogen peroxide induce CD18-mediated adhesion in the postischemic heart. Biochim Biophys Acta 1996;1316: 191-202   DOI   ScienceOn
18 Meduri GU, Kohler G, Headley S, Tolley E, Stentz F, Postlethwaite A. Inflammatory cytokines in the BAL of patients with ARDS: persistent elevation over time predicts poor outcome. Chest 1995;108:1303-14   DOI   ScienceOn
19 Yang KY, Arcaroli JJ, Abraham E. Early alterations in neutrophil activation are associated with outcome in acute lung injury. Am J Respir Crit Care Med 2003;167:1567-74   DOI
20 Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 2002;23:599-622   DOI   ScienceOn
21 Goodman RB, Strieter RM, Martin DP, Steinberg KP, Milberg JA, Maunder RJ, et al. Inflammatory cytokines in patients with persistence of the acute respiratory distress syndrome. Am J Respir Crit Care Med 1996;154:602-11   DOI
22 Rocksen D, Ekstrand-Hammarstrom B, Johansson L, Bucht A. Vitamin E reduces transendothelial migration of neutrophils and prevents lung injury in endotoxin-induced airway inflammation. Am J Respir Cell Mol Biol 2003;28:199-207   DOI   ScienceOn
23 Cao X, Phillis JW. The free radical scavenger, alpha-lipoic acid, protects against cerebral ischemiareperfusion injury in gerbils. Free Radic Res 1995;23: 365-70   DOI   ScienceOn
24 Blackwell TS, Blackwell TR, Holden EP, Christman BW, Christman JW. In vivo antioxidant treatment suppresses nuclear factor-kappa B activation and neutrophilic lung inflammation. J Immunol 1996;157: 1630-7
25 Abraham E, Carmody A, Shenkar R, Arcaroli J. Neutrophils as early immunologic effectors in hemorrhage- or endotoxemia-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol 2000;279:L1137-45   DOI
26 Ametov AS, Barinov A, Dyck PJ, Hermann R, Kozlova N, Litchy WJ, et al. The sensory symptoms of diabetic polyneuropathy are improved with alpha-lipoic acid: the SYDNEY trial. Diabetes Care 2003;26:770-6   DOI   ScienceOn