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The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-α and IL-1β Secretion  

Li, Tian Zhu (Konkuk University College of Veterinary Medicine)
Lee, Soo-Jin (Konkuk University College of Veterinary Medicine)
Park, Se-Jong (Konkuk University College of Veterinary Medicine)
Chang, Byung-Joon (Konkuk University College of Veterinary Medicine)
Lee, Jong-Hwan (Konkuk University College of Veterinary Medicine)
Kim, Kil-Soo (Kyungpook National University College of Veterinary Medicine)
Lee, Myoung-Heon (National Veterinary Research and Quarantine Service)
Choe, Nong-Hoon (Konkuk University College of Veterinary Medicine)
Publication Information
Tuberculosis and Respiratory Diseases / v.60, no.5, 2006 , pp. 554-563 More about this Journal
Abstract
Background: PM is known to induce various pulmonary diseases, including asthma, cancer, fibrosis and chronic bronchitis. Despite the epidemiological evidence the pathogenesis of PM-related pulmonary diseases is unclear. Methods: This study examined the effects of PM exposure on the secretion of $TNF-{\alpha}$ and $IL-1{\beta}$ in the cultured alveolar macrophages. The cultured primary alveolar macrophages were treated with the medium, PM ($5{\sim}20{\mu}g/cm^2$), LPS (5ng/ml), and PM with LPS for 24h and 48h respectively. ELISA was used to assay the secreted $TNF-{\alpha}$ and $IL-{\beta}$ in the culture medium. Western blotting was used to identify and determine the level of proteins isolated from the culture cells. The cells cultured in the $Lab-Tek^{(R)}$ chamber slides were stained with immunocytochemical stains. Results: PM induced $TNF-{\alpha}$ and $IL-1{\beta}$ secretion in the culturing alveolar macrophages, collected from the SPF and inflammatory rats. However, the effects were only dose-dependent in the inflammatory macrophages. When the cells were co-treated with PM and LPS, there was a significant synergistic effect compared with the LPS in the both cell types. Conclusion: PM might be play an important role in the induction and/or potentiation of various lung diseases by oversecretion of $TNF-{\alpha}$ and $IL-1{\beta}$.
Keywords
Particulate matter(PM); Alveolar macrophages; Tumor necrosis factor-$\alpha$ ($TNF-{\alpha}$); Interleukin $1{\beta}$ ($IL-1{\beta}$);
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