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Pretreatment of Diltiazem Ameliorates Endotoxin-Induced Acute Lung Injury by Suppression of Neutrophilic Oxidative Stress  

Jang, Yoo Suk (Department of Physiology, School of Medicine, Daegu Catholic University)
Lee, Young Man (Department of Physiology, School of Medicine, Daegu Catholic University)
Ahn, Wook Su (Department of Chest Surgery, School of Medicine, Daegu Catholic University)
Lee, Sang Chae (Department of Internal Medicine, School of Medicine, Daegu Catholic University)
Kim, Kyung Chan (Department of Internal Medicine, School of Medicine, Daegu Catholic University)
Hyun, Dae Sung (Department of Internal Medicine, School of Medicine, Daegu Catholic University)
Publication Information
Tuberculosis and Respiratory Diseases / v.60, no.4, 2006 , pp. 437-450 More about this Journal
Abstract
Background : Acute respiratory distress syndrome (ARDS) is characterized by severe inflammatory pulmonary edema of unknown pathogenesis. To investigate the pathogenesis of ARDS associated with neutrophilic oxidative stress, the role of phospholipase $A_2$ ($PLA_2$) was evaluated by the inhibition of calcium channel. Methods : In Sprague-Dawley rats, acute lung injury (ALI) was induced by the instillation of E.coli endotoxin (ETX) into the trachea. At the same time, diltiazem was given 60 min prior to tracheal instillation of ETX. Parameters of ALI such as lung and neutrophil $PLA_2$, lung myeloperoxidase (MPO), BAL neutrophils, protein, surfactant were measured. Production of free radicals from neutrophils was measured also. Morphological studies with light microscope and electron microscope were carried out and electron microscopic cytochemistry for detection of free radicals was performed also. Results : Diltiazem had decreased the ALI parameters effectively in ETX given rats and decreased the production of free radicals from neutrophils and lung tissues. Morphological studies denoted the protective effects of diltiazem. Conclusion : Diltiazem, a calcium channel blocker, was effective in amelioration of ALI by the suppression of neutrophilic oxidative stress mediated by $PLA_2$ activation.
Keywords
ARDS; $PLA_2$; Free Radicals; Neutrophils;
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