Browse > Article

Aquaporin in bleomycin induced lung injury  

Jang, An-Soo (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Park, Jong-Sook (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Lee, June-Hyuk (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Park, Sung-Woo (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Kim, Do-Jin (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Uh, Soo-Taek (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Kim, Yong-Hoon (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Park, Choon-Sik (Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital)
Publication Information
Tuberculosis and Respiratory Diseases / v.60, no.3, 2006 , pp. 330-336 More about this Journal
Abstract
Background : Aquaporins (AQPs) may play a role in the pathogenesis of pulmonary inflammation and edema. This study investigated the role ofAQPs in acute lung injury following bleomycin inhalation in rats. Methods : Sprague-Dawley rats were treated via inhalation with 10 U/kg bleomycin hydrochloride dissolved in 5 ml of normal saline. The control rats were treated with 5 ml normal saline. The animals (n = 6-8 rats per group) were sacrificed at 4, 7, and 14 d. The changes in AQP1, AQP4, and AQP5 expression levels over time were analyzed by Western blotting. The nitrate and nitrite concentrations in the bronchoalveolar lavage fluid (BALF) were measured using a modified Griess reaction. ELISA was used to check cytokines. Results : The respiration rates were significantly higher 4 and 7 days after the bleomycin treatment compared with those of the control rats. The tidal volume was lower in rats at 4 days after the bleomycin treatment, and the wet/dry weights of the lung were significantly higher than those of the control group. The nitrite and nitrate concentrations in the BALF from the rats at 4 days after exposure to bleomycin were greater than those from the saline-treated rats. Immunoblotting studies demonstrated that the AQP1 and AQP4 expression levels were lower in the rats at 4 days. However, the AQP4 expression level was higher at 7 days. The AQP5 expression level increased at 4, 7 and 14 days after the bleomycin treatment. Conclusion : This study demonstrates that AQPs are expressed differently in bleomycin-induced pulmonary edema.
Keywords
Aquaporin; Nitric oxides; acute lung injury;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Doyle RL, Szaflarski N, Modin GW, Wiener-Kronish JP, Matthay MA. Identification of patients with acute lung injury: predictors of mortality. Am J Respir Crit Care Med 1995;152:1818-24   DOI   ScienceOn
2 King LS, Nielsen S, Agre P. Aquaporin-1 water channel protein in lung: ontogeny, steroid-induced expression, and distribution in rat. J Clin Invest 1996;97:2183-91   DOI   ScienceOn
3 Nielsen S, King LS, Christensen BM, Arge P. Aquaporins in complex tissues: II. cellular and subcellular distribution in respiratory tract and glands of rat. Am J Physiol 1997;273:C1549-61   DOI
4 Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, et al. Measurement of protein using bicinchninic acid. Anal Biochem 1985; 150:76-85   DOI   ScienceOn
5 Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol Rev 1991;43:109-42
6 Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc Natl Acad Sci U S A 1991;88:11110-4   DOI   ScienceOn
7 The Acute Respiratory Distress Syndrome Network. Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome. N Engl J Med 2000;342:1301–8
8 Jang AS, Lee JU, Choi IS, Park KO, Lee JH, Park SW, et al. Expression of nitric oxide synthase, aquaporin 1 and aquaporin 5 in rat after bleomycin inhalation. Intensive Care Med 2004;30:489-95   DOI   ScienceOn
9 Brower RG, Lanken PN, MacIntyre N, Matthay MA, Morris A, Ancukiewicz M, et al. Higher versus lower positive end-expiratory pressures in patients with the acute respiratory distress syndrome. N Engl J Med 2004;351:327-36   DOI
10 Smith RE, Strieter RM, Zhang K, Phan SH, Standiford TJ, Lukacs NW, et al. A role for C-C chemokines in fibrotic lung disease. J Leukoc Biol 1995; 57:782-7   DOI
11 Agre P, Saboori AM, Asimos A, Smith BL. Purification and partial characterization of the Mr 30,000 integral membrane protein associated with the erythrocyte Rh(D) antigen. J Biol Chem 1987;262:17497-503
12 Jules-Elysee K, White DA. Bleomycin-induced pulm onary toxicity. Clin Chest Med 1990;11:1-20
13 Denker BM, Smith BL, Kuhajda FP, Agre P. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J Biol Chem 1988;263:15634-42
14 Towne JE, Harrod KS, Krane CM, Menon AG. Decreased expression of aquaporin (AQP) 1 and AQP5 in mouse lung after acute viral infection. Am J Respir Cell Mol Biol 2000;22:34-44   DOI   ScienceOn
15 Matthay MA, Zimmerman GA. Acute lung injury and the acute respiratory distress syndrome: four decades of inquiry into pathogenesis and rational management. Am J Respir Cell Mol Biol 2005;33:319-27   DOI   ScienceOn
16 Haddad IY, Pataki G, Hu P, Galliani C, Beckman JS, Matalon S. Quantification of nitrotyrosine levels in lung secretions of patients and animals with acute lung injury. J Clin Invest 1994;94:2407-13   DOI   ScienceOn
17 Piguet PF, Vesin C, Grau GE, Thompson RC. Inte rleukin 1 receptor antagonist (IL-1ra) prevents or cures pulmonary fibrosis elicited in mice by bleomycin or silica. Cytokine 1993;5:57-61   DOI   ScienceOn
18 Dickason RR, Huston MM, Huston DP. Enhanced detection of human interluekin-5 in biological fluids utilizing murine monoclonal antibodies which delineate didtinct neutralizing epitope. Cytokine 1994;6:647-56   DOI   ScienceOn
19 Ashbaugh DG, Bigelow DB, Petty TL, Levine BE. Acute respiratory distress in adults. Lancet 1967;2:319–23
20 Yang B, Brown D, Verkman AS. The mercurial insensitive water channel (AQP4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells. J Biol Chem 1996;271:4577-80   DOI
21 Preston GM, Carroll TP, Guggino WB, Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 1992;256:385-7.   DOI
22 Moncada S, Palmer RM, Higgs EA. Biosynthesis of nitric oxide from L-arginine: a pathway for the regulation of cell function and communication. Biochem Pharmacol 1989;38:1709-15   DOI   ScienceOn
23 Piguet PF, Collart MA, Grau GE, Kapanci Y, Vassalli P. Tumor necrosis factor/cachectin plays a key role in bleomycin-induced pneumopathy and fibrosis. J Exp Med 1989;170:655-63   DOI   ScienceOn
24 Jung JS, Preston GM, Smith BL, Guggino WB, Agre P. Molecular structure of the water channel through aquaporin CHIP. J Biol Chem 1994;269:14648-54
25 Zimmerman GA, Albertine KH, McIntyre TM. Pathogenesis of sepsis and septic-induced lung inury. In: Lenfant C, Matthay MA, editors. Acute respiratory distress syndrome. 1st ed. New York: Marcel Dekker; 2003. p. 245–87
26 van Hoek AN, Yang B, Kimiz S, Brown D. Freez- fracture analysis of plasma membrane of CHO cells stably expressing aquaporin1-5. J Membr Biol 1998;165:243-54   DOI