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The Comparison of the Effect of Cigarette and Stop Smoking-aiding Cigarette on Release of IL-6 from Bronchial Epithelial Cell  

Kim, Myoung Chan (Department of Internal Medicine, Seoul Adventist Hospital)
Jung, Jeil (Department of Internal Medicine, Seoul Adventist Hospital)
Jung, Jong Hoon (Department of Internal Medicine, WonKwang University, School of Medicine)
Kim, Hak Ryul (Department of Internal Medicine, WonKwang University, School of Medicine)
Yang, Sei Hoon (Department of Internal Medicine, WonKwang University, School of Medicine)
Jeong, Eun Taik (Department of Internal Medicine, WonKwang University, School of Medicine)
Kim, Hui Jung (WonKwang University, School of Medicine, Sanbon Medical center)
Publication Information
Tuberculosis and Respiratory Diseases / v.59, no.5, 2005 , pp. 530-535 More about this Journal
Abstract
Background and Aims : Cigarette smoking induces an inflammatory response in the airways, which may play a key role in the pathogenesis of chronic obstructive pulmonary disease. Interleukin-6 (IL-6) is one of the cytokines that plays an important role in inducing bronchial inflammation. The aim of this study was to determine if the level of the pro-inflammatory cytokine, Interleukin-6, is increased when the bronchial epithelial cells are exposed to a cigarette smoke extract (CSE) and an extract from stop smoking-aiding cigarettes, and examined the safety of these commercially available stop smoking-aiding cigarettes. Method : Bronchial epithelial cells were exposed to CSE from cigarette and stop smoking-aiding cigarettes for 24 hours. ELISA was used to measure the IL-6 levels in the supernatant from each condition. The IL-6 mRNA levels were measured by Taqman Real time RT-PCR. N-acetyl-L-cysteine(NAC) was added to each condition to determine if NAC can inhibit the release of IL-6 from the bronchial epithelial cells when they are exposed to CSE from cigarette and stop smoking-aiding cigarettes. Result : When bronchial epithelial cells were exposed to a CSE from cigarettes and stop smoking-aiding cigarettes, each type of CSE stimulated IL-6 production from the bronchial epithelial cells. The IL-6 mRNA level in the Bronchial epithelial cells was also elevated and NAC was found to inhibit the release of IL-6 from bronchial epithelial cells when they were exposed to the CSE from cigarettes and stop smoking-aiding cigarettes. Conclusion : Commercially available stop smoking-aiding cigarette can induce bronchial inflammation and can be harmful to smokers. Therefore, the safety of these cigarettes for smoking cessation should be evaluated.
Keywords
Cigarette; stop smoking-aiding cigarette; IL-6;
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1 Carpagnano GE, Kharitonov SA, Foschino-Barbaro MP, Resta O, Gramiccioni E, Barnes PJ. Increased inflammatory markers in the exhaled breath condensate ofcigarette smokers. Eur Respir J 2003;21:589-93   DOI   ScienceOn
2 Kelsen SG, Mardini IA, Zhou S, Benovic JL, Higgins NC. A technique to harvest viable tracheobronchial epithelial cells from living human donors. Am J Respir Cell Mol Biol 1992;7:66-72   DOI   ScienceOn
3 Carp H, Janoff A. Possible mechanisms of emphysema in smokers: in vitro suppression of serum elastaseinhibitory capacity by fresh cigarette smoke and its prevention by antioxidants. Am Rev Respir Dis 1978; 118:617-21
4 Kim HJ, Liu XI, Kohyama T, Conner H, Kohyama T, Rennard SI, et al. Reversible cigarette smoke extractinduced DNA damage in human lung fibroblasts. Am J Respir Cell Mol Biol 2004;31:483-90   DOI   ScienceOn
5 Sauty A, Manuel J, Philippeaux M, Leuenberg P. Cy􋹣 tostatic activity of alveolar macrophages from smokers and non-smokers: role of interleukin-1$\beta$<, interleukin-6 and tumor necrosis factor-TEX>$\alpha$ . Am J Respir Cell Mol Biol 1994;11:631-7   DOI   ScienceOn
6 Kim HJ, Liu XI, Wang H, Kohyama T, Kobayashi T, Rennard SI, et al. Glutathione prevents inhibition of fibroblast-mediated collagen gel contraction by cigarette smoke. Am J Physiol Lung Cell Mol Physiol 2002;283: L409-17   DOI
7 McCrea KA, Ensor JE, Nall K, Bleecker ER, Hasday JD. Altered cytokine regulation in the lungs of cigarette smokers. Am J Respir Crit Care Med 1994;150:696-703   DOI   ScienceOn
8 Kuschner WG, D'Alessandro A, Wong H , Blanc PD. Dose-dependent cigarette smoking-related inflammatory responses in healthy adults. Eur Respir J 1996;9: 1989-94   DOI   ScienceOn
9 Barnes PJ, Stockley RA. COPD: current therapeutic interventions and future approaches. Eur Respir J 2005;25:1084-106   DOI   ScienceOn
10 Patel IS, Roberts NJ, Lloyd-Owen SJ, Sapsford RJ, Wedzicha JA. Airway epithelial inflammatory responses and clinical parameters in COPD. Eur Respir J 2003; 22:94-9   DOI   ScienceOn
11 Carnevali S, Nakamura Y, Mio T, Liu XI, Takigawa K, Rennard SI, et al. Cigarette smoke extract inhibits fibroblast-mediated collagen gel contraction. Am J Physiol 1998;274:L591-8