Browse > Article
http://dx.doi.org/10.11620/IJOB.2018.43.3.147

Participation of Opioid Pathway in the Central Antinociceptive Effects of Eugenol  

Kang, Song-hee (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Kang, Sa-won (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Kim, Jae-ho (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Kim, Hee-young (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Ryu, Hyeon-seo (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Bae, So-yeon (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Oh, Ju-ae (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Lee, Jun-hyuk (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Hyun, Ji-hee (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Ahn, Dong Kuk (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
Publication Information
International Journal of Oral Biology / v.43, no.3, 2018 , pp. 147-153 More about this Journal
Abstract
The aim of the present study was to evaluate the central antinociceptive effects of eugenol after intraperitoneal administration. Experiments were carried out using male Sprague-Dawley rats. Subcutaneous injection of 5% formalin-induced nociceptive behavioral responses was used as the pain model. Subcutaneous injection of 5% formalin significantly produced nociceptive responses by increasing the licking time during nociceptive behavior. Subsequent intraperitoneal injection of 100 mg/kg of eugenol led to a significant decrease in the licking time. However, low dose of eugenol (50 mg/kg) did not affect the nociceptive behavioral responses produced by subcutaneous injection of formalin. Intrathecal injection of $30{\mu}g$ of naloxone, an opioid receptor antagonist, significantly blocked antinociceptive effects produced by intraperitoneal injection of eugenol. Neither intrathecal injection of methysergide ($30{\mu}g$), a serotonin receptor antagonist nor phentolamine ($30{\mu}g$), an ${\alpha}-adrenergic$ receptor antagonist influenced antinociceptive effects of eugenol, as compared to the vehicle treatment. These results suggest that central opioid pathway participates in mediating the antinociceptive effects of eugenol.
Keywords
eugenol; antinociception; formalin; pain; opioid;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Li W, Tsubouchi R, Qiao S, Haneda M, Murakami K, Yoshino M. Inhibitory action of eugenol compounds on the production of nitric oxide in RAW264.7 macrophages. Biomed Res. 2006;27:69-74. doi: https://doi.org/10.2220/biomedres.27.69.   DOI
2 Kim SS, Oh OJ, Min HY, Park EJ, Kim Y, Park HJ, Nam Han Y, Lee SK. Eugenol suppresses cyclooxygenase-2 expression in lipopolysaccharide-stimulated mouse macrophage RAW 264.7 cells. Life Sci. 2003;73:337-348. doi:https://doi.org/10.1016/S0024-3205(03)00288-1.   DOI
3 Briseno BM, Willershausen B. Root canal sealer cytotoxicity on human gingival fibroblasts. 1. Zinc oxide-eugenol-based sealers. J Endod. 1990;16:383-386. doi:https://doi.org/10.1016/S0099-2399(06)81910-2.   DOI
4 Goerig AC, Payne TF, del Rio CE. The pulpal response to ZOE with stock eugenol versus ZOE with purified eugenol. Oral Surg Oral Med Oral Pathol. 1980 ;50:557-562.   DOI
5 Horsted P, El Attar K, Langeland K. Capping of monkey pulps with Dycal and a Ca-eugenol cement. Oral Surg Oral Med Oral Pathol. 1981;52:531-553.   DOI
6 Koh T, Murakami Y, Tanaka S, Machino M, Sakagami H. Re-evaluation of anti-inflammatory potential of eugenol in IL-$1{\beta}$-stimulated gingival fibroblast and pulp cells. In Vivo. 2013;27:269-273. doi:http://iv.iiarjournals.org/content/27/2/269.long.
7 Wie MB, Won MH, Lee KH, Shin JH, Lee JC, Suh HW, Song DK, Kim YH. Eugenol protects neuronal cells from excitotoxic and oxidative injury in primary cortical cultures. Neurosci Lett. 1997;225:93-96. doi: https://doi.org/10.1016/S0304-3940(97)00195-X.   DOI
8 Hansen EK, Asmussen E. Influence of temporary filling materials on effect of dentin-bonding agents. Scand J Dent Res. 1987;95:516-520.
9 Anpo M, Shirayama K, Tsutsui T. Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells. Odontology. 2011;99:188-192. doi:10.1007/s10266-011-0009-2.   DOI
10 Fujisawa S, Atsumi T, Satoh K, Sakagami H. Interaction between 2-ethoxybenzoic acid (EBA) and eugenol, and related changes in cytotoxicity. J Dent Res. 2003;82:43-47.   DOI
11 Irie Y, Keung WM. Rhizoma acori graminei and its active principles protect PC-12 cells from the toxic effect of amyloid- ${\beta}$ peptide. Brain Res. 2003;963:282-289. doi:https://doi.org/10.1016/S0006-8993(02)04050-7.   DOI
12 Won MH, Lee JC, Kim YH, Song DK, Suh HW, Oh YS, Kim JH, Shin TK, Lee YJ, Wie MB. Postischemic hypothermia induced by eugenol protects hippocampal neurons from global ischemia in gerbils. Neurosci Lett. 1998;254:101-104. doi:https://doi.org/10.1016/S0304-3940(98)00664-8.   DOI
13 Nangle MR, Gibson TM, Cotter MA, Cameron NE. Effects of eugenol on nerve and vascular dysfunction in streptozotocin- diabetic rats. Planta Med. 2006;72:494-500. doi:10.1055/s-2005-916262.   DOI
14 Brodin P. Differential inhibition of A, B and C fibres in the rat vagus nerve by lidocaine, eugenol and formaldehyde. Arch Oral Biol. 1985;30:477-480.   DOI
15 Norte MC, Cosentino RM, Lazarini CA. Effects of methyl- eugenol administration on behavioral models related to depression and anxiety, in rats. Phytomedicin.2005;12: 294-298. doi:https://doi.org/10.1016/j.phymed.2003.12.007.   DOI
16 Jeon HJ, Han SR, Lim KH, Won KA, Bae YC, Ahn DK. Intracisternal administration of NR2 subunit antagonists attenuates the nociceptive behavior and p-p38 MAPK expression produced by compression of the trigeminal nerve root. Mol Pain. 2011;7:46. doi: 10.1186/1744-8069-7-46.
17 Won KA, Kim MJ, Yang KY, Park JS, Lee MK, Park MK, Bae YC, Ahn DK. The glial-neuronal GRK2 pathway participates in the development of trigeminal neuropathic pain in rats. J Pain. 2014;15:250-261. doi: 10.1016/j.jpain.2013.10.013.   DOI
18 Jorkjend L, Skoglund LA. Effect of non-eugenol- and eugenol-containing periodontal dressings on the incidence and severity of pain after periodontal soft tissue surgery. J Clin Periodontol. 1990;17:341-344. doi:https://doi.org/10.1111/j.1600-051X. 1990.tb00028.x.   DOI
19 Hodosh AJ, Hodosh S, Hodosh M. Potassium nitrate-zinc oxide eugenol temporary cement for provisional crowns to diminish postpreparation tooth pain. J Prosthet Dent. 1993; 70:493-495. doi:https://doi.org/10.1016/0022-3913(93)90260-U.   DOI
20 Li HY, Lee BK, Kim JS, Jung SJ, Oh SB. Eugenol Inhibits ATP-induced P2X Currents in Trigeminal Ganglion Neurons. Korean J Physiol Pharmacol. 2008 ;12:315-321. doi:10.4196/kjpp.2008.12.6.315.   DOI
21 Kozam G. The effect of eugenol on nerve transmission. Oral Surg Oral Med Oral Pathol. 1977;44:799-805.   DOI
22 Carlini EA, Dallmeier K, Zelger JL. Methyleugenol as a surgical anesthetic in rodents. Experientia. 1981;37:588-589   DOI
23 Sell AB, Carlini EA. Anesthetic action of methyleugenol and other eugenol derivatives. Pharmacology. 1976;14:367-377. doi:https://doi.org/10.1159/000136617.   DOI
24 Guenette SA, Beaudry F, Marier JF, Vachon P. Pharmacokinetics and anesthetic activity of eugenol in male Sprague-Dawley rats. J Vet Pharmacol Ther. 2007;30:91-92. doi:https://doi.org/ 10.1111/j.1365-2885.2007.00814.x.   DOI
25 Yano S, Suzuki Y, Yuzurihara M, Kase Y, Takeda S, Watanabe S, Aburada M, Miyamoto K. Antinociceptive effect of methyleugenol on formalin-induced hyperalgesia in mice. Eur J Pharmacol. 2006;553:99-103. doi:https://doi.org/10.1016/j.ejphar.2006.09.020.   DOI
26 Wang ZJ, Tabakoff B, Levinson SR, Heinbockel T. Inhibition of Nav1.7 channels by methyl eugenol as a mechanism underlying its antinociceptive and anesthetic actions. Acta Pharmacol Sin. 2015;36:791-799. doi:10.1038/aps.2015.26.   DOI
27 Chung G, Im ST, Kim YH, Jung SJ, Rhyu MR, Oh SB. Activation of transient receptor potential ankyrin 1 by eugenol. Neuroscience. 2014;261:153-160. doi: https://doi.org/10.1016/j.neuroscience.2013.12.047   DOI
28 Park CK, Kim K, Jung SJ, Kim MJ, Ahn DK, Hong SD, Kim JS, Oh SB. Molecular mechanism for local anesthetic action of eugenol in the rat trigeminal system. Pain. 2009;144:84-94. doi:10.1016/j.pain.2009.03.016. Epub 2009 Apr 18.   DOI
29 Basbaum AI, Fields HL. Endogenous pain control mechanisms: review and hypothesis. Ann Neurol. 1978;4:451-462. doi: https://doi.org/10.1002/ana.410040511.   DOI
30 Cho JS, Kim TH, Lim JM, Song JH. Effects of eugenol on Na+ currents in rat dorsal root ganglion neurons. Brain Res. 2008;1243:53-62. doi: https://doi.org/10.1016/j.brainres.2008.09.030.   DOI
31 Basbaum AI, Fields HL. Endogenous pain control systems: brainstem spinal pathways and endorphin circuitry. Annu Rev Neurosci. 1984;7:309-338. doi:https://doi.org/10.1146/annurev.ne.07.030184.001521.   DOI
32 Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. The formalin test: an evaluation of the method. Pain. 1992;51:5-17. doi:10.1016/0304-3959(92)90003-T.   DOI
33 Choi HS, Ju JS, Lee HJ, Jung CY, Kim BC, Park JS, Ahn DK. Effects of TNF-alpha injected intracisternally on the nociceptive jaw-opening reflex and orofacial formalin test in freely moving rats. Prog Neuropsychopharmacol Biol Psychiatry. 2003;27:613-618. doi:https://doi.org/10.1016/S0278-5846(03)00049-6.   DOI
34 Reynolds DV. Surgery in the rat during electrical analgesia induced by focal brain stimulation. Science. 1969;164:444-445. doi: 10.1126/science.164.3878.444.   DOI
35 Mayer DJ, Wolfle TL, Akil H, Carder B, Liebeskind JC. Analgesia from electrical stimulation in the brainstem of the rat. Science. 1971 ;174:1351-1354. doi:10.1126/science.174.4016.1351   DOI
36 Choi HS, Ju JS, Lee HJ, Kim BC, Park JS, Ahn DK. Effects of intracisternal injection of interleukin-6 on nociceptive jaw opening reflex and orofacial formalin test in freely moving rats. Brain Res Bull. 2003;59:365-370. doi:https://doi.org/10.1016/S0361-9230(02)00931-0.   DOI
37 Raboisson P, Dallel R. The orofacial formalin test. Neurosci Biobehav Rev. 2004;28:219-226. doi:https://doi.org/10.1016/ j.neubiorev.2003.12.003.   DOI
38 Ahn DK, Lee KR, Lee HJ, Kim SK, Choi HS, Lim EJ, Park JS. Intracisternal administration of chemokines facilitated formalin-induced behavioral responses in the orofacial area of freely moving rats. Brain Res Bull. 2005;66:50-58. doi: https://doi.org/10.1016/j.brainresbull.2005.03.015.   DOI
39 Yang GY, Woo YW, Park MK, Bae YC, Ahn DK, Bonfa E. Intracisternal administration of NR2 antagonists attenuates facial formalin-induced nociceptive behavior in rats. J Orofac Pain. 2010;24:203-211.
40 Raboisson P, Dallel R, Clavelou P, Sessle BJ, Woda A. Effects of subcutaneous formalin on the activity of trigeminal brain stem nociceptive neurones in the rat. J Neurophysiol. 1995;73:496-505. doi:10.1152/jn.1995.73.2.496.   DOI
41 Abbott FV, Franklin KB, Westbrook RF. The formalin test: scoring properties of the first and second phases of the pain response in rats. Pain. 1995;60:91-102.   DOI
42 Watkins LR, Mayer DJ. Organization of endogenous opiate and nonopiate pain control systems. Science. 1982;216: 1185-1192. doi:10.1126/science.6281891.   DOI
43 Llewelyn MB, Azami J, Roberts MH. The effect of modification of 5-hydroxytryptamine function in nucleus raphe magnus on nociceptive threshold. Brain Res. 1984;306: 165-170. doi:https://doi.org/10.1016/0006-8993(84)90365-2.   DOI
44 Ossipov MH, Chatterjee TK, Gebhart GF. Locus coeruleus lesions in the rat enhance the antinociceptive potency of centrally administered clonidine but not morphine. Brain Res. 1985;341:320-330. doi:https://doi.org/10.1016/0006-8993(85) 91071-6.   DOI
45 Simson PE, Weiss JM. Alpha-2 receptor blockade increases responsiveness of locus coeruleus neurons to excitatory stimulation. J Neurosci. 1987;7:1732-1740. doi:https://doi.org/10.1523/JNEUROSCI.07-06-01732.1987.   DOI
46 Ohkubo T, Shibata M. The selective capsaicin antagonist capsazepine abolishes the antinociceptive action of eugenol and guaiacol. J Dent Res. 1997;76:848-851. doi:https://doi.org/10.1177/00220345970760040501.   DOI