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http://dx.doi.org/10.11620/IJOB.2017.42.4.197

Capillary Electrophoresis Detection of Hydrogen Peroxide by Using Titanium Ion and 4-(2-thiazolylazo)resorcinol  

Vu Phuong, Dong (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University)
Yoo, Hoon (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University)
Publication Information
International Journal of Oral Biology / v.42, no.4, 2017 , pp. 197-201 More about this Journal
Abstract
A novel method for the detection of hydrogen peroxide in aqueous solution was developed via reaction between $H_2O_2$, trivalent titanium ion ($Ti^{3+}$) and 4-(2-thiazolylazo) resorcinol (TAR), resulting in a ternary complex with a maximum UV absorbance at 530 nm. The CE detection of $H_2O_2$ was fast, sensitive and cost-effective without pretreatment procedures. $H_2O_2$ was detected within 15 min at 1 to $100{\mu}M$ range with the lowest detection limit at $1.0{\mu}M$. Under the optimized CE conditions, the concentration of $H_2O_2$ in coffee or tea extract was quantitatively determined. Our results show that CE detection of the ternary complex of $H_2O_2-Ti^{3+}$-TAR has potential applications for the detection of $H_2O_2$ in aqueous sources.
Keywords
Hydrogen peroxide; capillary electrophoresis (CE); titanium ion ($Ti^{3+}$); 4-(2-thiazolylazo)resorcinol (TAR);
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1 Tahirovic A, Copra A, Omanovic-Miklicanin E, Kalcher K . A chemiluminescence sensor for the determination of hydrogen peroxide. Talanta. 2007;72: 1378-1385. doi: https://doi.org/10.1016/j.talanta.2007.01.072.   DOI
2 Georgiou G, Masip L. An Overoxidation Journey with a Return Ticket. Science. 2003;5619: 592-594. doi: 10.1126/science.1084976.
3 Rhee SG. $H_{2}O_{2}$, a Necessary Evil for Cells Signaling. Science. 2006;312: 1882-1884. doi: 10.1126/science.1130481.   DOI
4 Tredwin CJ, Naik S, Lewis NJ, Scully C. Hydrogen peroxide tooth-whitening (bleaching) products: Review of adverse effects and safety issues. Br. Dent. J. 2006;200: 371-376. doi: 10.1038/sj.bdj.4813423.   DOI
5 Matsubara C, Kudo K, Kawashita T, Takamura K. Spectrophotometric Determination of Hydrogen Peroxide with Titanium 2-((5-Bromopyridyl)azo)-5-(N-Propyl-NSulfopropylamino) Phenol Reagent and Its Application to the Determination of Serum Glucose Using Glucose Oxidase. Anal. Chem. 1985;57: 1107-1109. doi: 10.1021/ac00283a032.   DOI
6 Simon PW. Ferrous Ion Oxidation in Presence of Ferric Ion Indicator Xylenol Orange for Measurement of Hydroperoxides. Methods Enzymol. 1994;233: 182-189. doi: https://doi.org/10.1016/S0076-6879(94)33021-2.
7 Tanner PA, Wong AYS. Spectrophotometric determination of hydrogen peroxide in rainwater. Anal. Chim. Acta 1998;370:279-287. doi: https://doi.org/10.1016/S0003-2670(98)00273-6.   DOI
8 Komazaki Y, Inoue T, Tanaka S. Automated measurement system for H2O2 in the atmosphere by diffusion scrubber sampling and HPLC analysis of Ti(IV)-PAR-H2O2 complex. Analyst 2001;126: 587-593.   DOI
9 Miller EW, Tulyathan O, Isacoff EY, Chang CJ. Molecular Imaging of Hydrogen Peroxide Produced for Cell Signaling. Nat. Chem. Biol. 2007;3: 263-267. doi:10.1038/nchembio871.
10 Navas MJ, Jimenez AM, Galan G. Air analysis: determination of hydrogen peroxide by chemiluminescence. Atmos. Environ. 1999;33: 2279-2283. doi: https://doi.org/10.1016/S1352-2310(98)00117-4.   DOI
11 Klein-Szanto AJP, Slaga TJ. Effects on peroxides on rodent skin: Epidermal hyperplasia and tumor promotion. J. Invest. Dermatol. 1982;79: 30-34. doi: https://doi.org/10.1111/1523-1747.ep12510444.   DOI
12 Ping J, Wu J, Fan K, Ying Y. An amperometric sensor based on Prussian blue and poly(o-phenylenediamine) modified glassy carbon electrode for the determination of hydrogen peroxide in beverages. Food Chem. 2011;126: 2005-2009. doi: 10.1016/j.foodchem.2010.12.073.   DOI
13 Matsubara C, Iwamoto T, Nishikawa Y, Takamura K, Yano S, Yoshikawa S. Coloured species formed from the titanium (IV)-4-(2'-pyridylazo) resorcinol reagent in the spectrophotometric determination of trace amounts of hydrogen peroxide. J. Chem. Soc. Dalton. Trans. 1985;1:81-84. doi: 10.1039/DT9850000081.
14 Akagawa M, Shigemitsu T, Suyama K. Production of Hydrogen Peroxide by Polyphenols and Polyphenol-rich Beverages under Quasi-physiological Conditions. Biosci. Biotechnol. Biochem. 2003;67: 2632-2640. doi: 10.1271/bbb.67.2632.   DOI