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http://dx.doi.org/10.11620/IJOB.2017.42.4.169

Distribution of Cold Receptor Transient Receptor Potential Melastatin 8-Immunopositive Axons in the Mouse Dental Pulp and Periodontal Tissue  

Kim, Tae Heon (Department of Dental Hygiene, Taegu Science University)
Lee, Jae Sik (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University)
Kim, Yun Sook (Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University)
Bae, Yong Chul (Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University)
Publication Information
International Journal of Oral Biology / v.42, no.4, 2017 , pp. 169-174 More about this Journal
Abstract
Transient receptor potential melastatin 8 (TRPM8) plays a crucial role in innocuous cool sensation, acute cold pain and cold-induced hyperalgesia during pathologic conditions. To help understand TRPM8-mediated cold perception in the dental pulp and periodontal tissues, we examined the distribution of TRPM8-immunopositive (+) axons in molar and incisor pulp and periodontal tissues using transgenic mice expressing a genetically encoded axonal tracer in TRPM8+ neurons. In the radicular pulp of the molar teeth, a small number of TRPM8+ axons were observed. TRPM8+ axons branched frequently and extensively in the core of coronal pulp, forming a network in the peripheral pulp. Some TRPM8+ axons ascended between odontoblasts and were observed in the dentinal tubule. TRPM8+ axons were linear-shaped in the radicular pulp, whereas many TRPM8+ axons showed portions shaped like beads connected with thin axonal stands at the peripheral pulp. TRPM8 was densely expressed in the bead portions. In the incisor pulp, TRPM8+ axons were occasionally observed in the core of the coronal pulp and rarely observed at the peripheral pulp. TRPM8+ axons were occasionally observed and showed a linear shape rather than a bead-like appearance in the periodontal ligament and lamina propria of the gingival tissue. These findings, showing differential distribution of TRPM8+ axons between radicular and coronal portions of the molar pulp, between incisor and molar pulp, and between dental pulp and periodontal tissues, may reflect differential cold sensitivity in these regions.
Keywords
TRPM8; dental pulp; periodontal tissue; immunohistochemistry;
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1 Colburn RW, Lubin ML, Stone DJ Jr, Wang Y, Lawrence D, D'Andrea MR, Brandt MR, Liu Y, Flores CM, Qin N. Attenuated cold sensitivity in TRPM8 null mice. Neuron. 2007;54(3):379-386. doi:10.1016/j.neuron.2007.04.017.   DOI
2 Kim TH, Park SK, Choi SY, Lee JS, Bae YC. Morphologic Change of Parvalbumin-positive Myelinated Axons in the Human Dental Pulp. J Endod. 2017;43(6):977-981. doi: 10.1016/j.joen.2017.01.010.   DOI
3 Byers MR. Dental sensory receptors. Int Rev Neurobiol. 1984;25:39-94.
4 Kato J, Ichikawa H, Wakisaka S, Matsuo S, Sakuda M, Akai M. The distribution of vasoactive intestinal polypeptides and calcitonin gene-related peptide in the periodontal ligament of mouse molar teeth. Arch Oral Biol. 1990;35(1):63-66. doi: https://doi.org/10.1016/0003-9969(90)90116-R.   DOI
5 Saito I, Ishii K, Hanada K, Sato O, Maeda T. Responses of calcitonin gene-related peptide-immunopositive nerve fibres in the periodontal ligament of rat molars to experimental tooth movement. Arch Oral Biol. 1991;36(9):689-692. doi: https://doi.org/10.1016/0003-9969(91)90023-N.   DOI
6 Mengel MK, Jyvasjarvi E, Kniffki KD. Identification and characterization of afferent periodontal A delta fibres in the cat. J Physiol. 1993;464:393-405. doi:10.1113/jphysiol.1993.sp019641.   DOI
7 Ochi K, Wakisaka S, Youn SH, Hanada K, Maeda T. Calretinin-like immunoreactivity in the Ruffini endings, slowly adapting mechanoreceptors, of the periodontal ligament of the rat incisor. Brain Res. 1997;769(1):183-187. doi:https://doi.org/10.1016/S0006-8993(97)00847-0.   DOI
8 Alkhamrah BA, Hoshino N, Kawano Y, Harada F, Hanada K, Maeda T. The periodontal Ruffini endings in brain derived neurotrophic factor (BDNF) deficient mice. Arch Histol Cytol. 2003;66(1):73-81. doi:http://doi.org/10.1679/aohc.66.73.   DOI
9 Fremeau RT Jr, Voglmaier S, Seal RP, Edwards RH. VGLUTs define subsets of excitatory neurons and suggest novel roles for glutamate. Trends Neurosci. 2004;27(2):98-103. doi: 10.1016/j.tins.2003.11.005.   DOI
10 Rahman F, Harada F, Saito I, Suzuki A, Kawano Y, Izumi K, Nozawa-Inoue K, Maeda T. Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors. Neurosci Lett. 2011;488(2):173-177. doi:10.1016/j.neulet.2010.11.023.   DOI
11 Takashima Y, Daniels RL, Knowlton W, Teng J, Liman ER, McKemy DD: Diversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 Neurons. J Neurosci. 2007;27(51):14147-14157. doi:10.1523/JNEUROSCI.4578-07.2007.   DOI
12 Knowlton WM1, McKemy DD. TRPM8: from cold to cancer, peppermint to pain. Curr Pharm Biotechnol. 2011;12(1):68-77. doi:10.2174/138920111793937961.   DOI
13 Brumovsky P, Watanabe M, Hokfelt T. Expression of the vesicular glutamate transporters-1 and -2 in adult mouse dorsal root ganglia and spinal cord and their regulation by nerve injury. Neuroscience. 2007;147(2):469-490. doi:10. 1016/j.neuroscience.2007.02.068.   DOI
14 Yang ES, Jin MU, Hong JH, Kim YS, Choi SY, Kim TH, Cho YS, Bae YC. Expression of vesicular glutamate transporters VGLUT1 and VGLUT2 in the rat dental pulp and trigeminal ganglion following inflammation. PLoS One. 2014;9(10):e109723. doi:10.1371/journal.pone.0109723.   DOI
15 Paik SK, Kim SK, Choi SJ, Yang ES, Ahn SH, Bae YC. Vesicular glutamate transporters in axons that innervate the human dental pulp. J Endod. 2012;38(4):470-474. doi: 10.1016/j.joen.2011.12.012.   DOI
16 Kim YS, Kim YJ, Paik SK, Cho YS, Kwon TG, Ahn DK, Kim SK, Yoshida A, Bae YC. Expression of metabotropic glutamate receptor mGluR5 in human dental pulp. J Endod. 2009;35(5):690-694. doi:10.1016/j.joen.2009.02.005.   DOI
17 Cho YS, Kim YS, Moozhayil SJ, Yang ES, Bae YC. The expression of hyperpolarization-activated cyclic nucleotidegated channel 1 (HCN1) and HCN2 in the rat trigeminal ganglion, sensory root, and dental pulp. Neuroscience. 2015;291:15-25. doi:10.1016/j.neuroscience.2015.01.066.   DOI
18 Peier AM, Moqrich A, Hergarden AC, Reeve AJ, Andersson DA, Story GM, Earley TJ, Dragoni I, McIntyre P, Bevan S, Patapoutian A. A TRP channel that senses cold stimuli and menthol. Cell. 2002;108(5):705-715. doi:https://doi.org/10.1016/S0092-8674(02)00652-9.   DOI
19 McKemy DD, Neuhausser WM, Julius D. Identification of a cold receptor reveals a general role for TRP channels in thermosensation. Nature. 2002;416(6876):52-58. doi:10.1038/nature719.   DOI
20 Chuang HH1, Neuhausser WM, Julius D. The super-cooling agent icilin reveals a mechanism of coincidence detection by a temperature-sensitive TRP channel. Neuron. 2004;43(6):859-869. doi:10.1016/j.neuron.2004.08.038.   DOI
21 Kim YS, Park JH, Choi SJ, Bae JY, Ahn DK, McKemy DD, Bae YC. Central connectivity of transient receptor potential melastatin 8-expressing axons in the brain stem and spinal dorsal horn. PLoS One. 2014;9(4):e94080. doi:10.1371/journal.pone.0094080.   DOI
22 Bodding M. TRP proteins and cancer. Cell Signal. 2007;19(3):617-624. doi:10.1016/j.cellsig.2006.08.012.   DOI
23 Wrigley PJ, Jeong HJ, Vaughan CW. Primary afferents with TRPM8 and TRPA1 profiles target distinct subpopulations of rat superficial dorsal horn neurones. Br J Pharmacol. 2009;157(3):371-380. doi:10.1111/j.1476-5381.2009.00167.x.   DOI
24 Bautista DM, Siemens J, Glazer JM, Tsuruda PR, Basbaum AI, Stucky CL, Jordt SE, Julius D. The menthol receptor TRPM8 is the principal detector of environmental cold. Nature. 2007;448(7150):204-208. doi:10.1038/nature05910.   DOI
25 Knowlton WM, Bifolck-Fisher A, Bautista DM, McKemy DD. TRPM8, but not TRPA1, is required for neural and behavioral responses to acute noxious cold temperatures and cold-mimetics in vivo. Pain. 2010;150(2):340-350. doi: 10.1016/j.pain.2010.05.021.   DOI
26 Kim YS, Kim TH, McKemy DD, Bae YC. Expression of vesicular glutamate transporters in transient receptor potential melastatin 8 (TRPM8)-positive dental afferents in the mouse. Neuroscience. 2015;303:378-388. doi:10.1016/j.neuroscience.2015.07.013.   DOI
27 Tsavaler L, Shapero MH, Morkowski S, Laus R. Trp-p8, a novel prostate-specific gene, is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins. Cancer Res. 2001;61(9):3760-3769.
28 Dhaka A, Murray AN, Mathur J, Earley TJ, Petrus MJ, Patapoutian A. TRPM8 is required for cold sensation in mice. Neuron. 2007;54(3):371-378. doi:10.1016/j.neuron.2007.02.024.   DOI