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http://dx.doi.org/10.11620/IJOB.2015.40.2.079

Antimicrobial Effect of Coptidis rhizome Extract against Mutans Streptococci and Periodontopathogens  

Park, Soon-Nang (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Lim, Yun Kyong (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Kook, Joong-Ki (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Publication Information
International Journal of Oral Biology / v.40, no.2, 2015 , pp. 79-83 More about this Journal
Abstract
The purpose of the study was to investigate the antimicrobial activity of the methanol extract of Coptidis rhizome against the type strains of cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus, and the periodontopathogens, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extract and the methanol extract fractions of Coptidis rhizome separated by silica gel chromatography were evaluated by determining the minimal bactericidal concentration (MBC) values, using the microdilution method. The cell viability test of the extracts of Coptidis rhizome on the KB cells was also studied by methyl thiazolyl tetrazolium (MTT) assay. Our results showed that the 11th fraction (F11) of the methanol extract had the greatest antimicrobial activity against the tested bacteria, with no associated cytotoxicity on the KB cells, upto a concentration of $50{\mu}g/ml$. These results suggest that the silica gel chromatography fraction F11 of the methanol extract of Coptidis rhizome, could be useful in the development of oral hygiene products as an antimicrobial agent for the prevention of dental caries and periodontal diseases.
Keywords
antimicrobial effect; Coptidis rhizome; mutans streptococci; periodontopathogens;
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Times Cited By KSCI : 4  (Citation Analysis)
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