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http://dx.doi.org/10.11620/IJOB.2014.39.2.075

Antimicrobial Effects of Oleanolic Acid, Ursolic Acid, and Sophoraflavanone G against Enterococcus faecalis and Propionibacterium acnes  

Jo, Eojin (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Choi, Mi-Hwa (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Kim, Hwa-Sook (Department of Dental Hygiene, Chunnam Techno University)
Park, Soon-Nang (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Lim, Yun Kyong (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Kang, Christina K. (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Kook, Joong-Ki (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
Publication Information
International Journal of Oral Biology / v.39, no.2, 2014 , pp. 75-79 More about this Journal
Abstract
The aim of this study was to investigate antimicrobial effect of oleanolic acid (OA), ursolic acid (UA), and sophoraflavanone G against Enterococcus faecalis and Propionibacterium acnes, which are the major causative bacteria of endodontic infections. The antimicrobial activity was evaluated by the minimal inhibitory concentration (MIC). The data showed that the OA, UA, and sophoraflavanone G had antimicrobial effect on all the strains use in the study with $16-64{\mu}g/ml$, $8-64{\mu}g/ml$, and $1-8{\mu}g/ml$ of MIC values, respectively. These results indicate that OA, UA, and sophoraflavanone G could be useful in the development of antiseptic solution for washing the root canal in endodontic treatments.
Keywords
antimicrobial effect; oleanolic acid; ursolic acid; sophoraflavanone G; Enterococcus faecalis; Propionibacterium acnes;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Menezes MM, Valera MC, Jorge AO, Koga-Ito CY, Camargo CH, Mancini MN. In vitro evaluation of the effectiveness of irrigants and intracanal medicaments on microorganisms within root canals. Int Endod J. 2004;37:311-319.   DOI
2 Siqueira JF, Rocas IN. Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod. 2008;34:1291-1301.   DOI
3 Walker A. Definite and dependable therapy for pulpless teeth. J Am Dent Assoc. 1936;23:1418.
4 Senia ES, Marshall FJ, Rosen S. The solvent action of sodium hypochlorite on pulp tissue of extracted teeth. Oral Surg Oral Med Oral Pathol. 1971;31:96-103.   DOI   ScienceOn
5 Siqueira JF Jr, Batista MM, Fraga RC, de Uzeda M. Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria. J Endod. 1998;24:414-416.   DOI   ScienceOn
6 Mehdipour O, Kleier DJ, Averbach RE. Anatomy of sodium hypochlorite accidents. Compend Contin Educ Dent. 2007;28:544-546.
7 Zamany A, Safavi K, Spangberg LS. The effect of chlorhexidine as an endodontic disinfectant. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96:578-581.   DOI
8 Naenni N, Thoma K, Zehnder M. Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod. 2004;30:785-787.   DOI
9 Schaupp H, Wohnaut H. Disturbances of taste from oral disinfectants. HNO. 1978;26:335-341.
10 Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:86-93.   DOI   ScienceOn
11 Jett BD, Huycke MM, Gilmore MS. Virulence of enterococci. Clin Microbiol Rev. 1994;7:462-478.   DOI
12 Evanes M, Davies K, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 2002;35:221-228.   DOI   ScienceOn
13 Rocas IN, Siqueira JF Jr. Comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. J Endod. 2011;37:143-150.   DOI
14 Siqueira JF Jr, Magalhaes KM, Rocas IN. Bacterial reduction in infected root canals treated with 2.5% NaOCl as an irrigant and calcium hydroxide/camphorated paramonochlorophenol paste as an intracanal dressing. J Endod. 2007;33:667-672.   DOI
15 Fujii R, Saito Y, Tokura Y, Nakagawa KI, Okuda K, Ishihara K. Characterization of bacterial flora in persistent apical periodontitis lesions. Oral Microbiol Immunol. 2009;24:502-505.   DOI
16 Kubo I, Muroi H, Himejima M. Antimicrobial activity of green tea flavor components and their combination effects. J Agri Food Chem. 1992;40:245-248.   DOI
17 Lee ES, Ahn TY, Yoon JJ, Kook JK, Lee BR, Kim DK. Restraint effect on leaf-extract from Camellia sinensis and seed-extract from Casia tora against periodontopathogens. J Korean Acad Dent Health 2003;27:569-579.
18 Lim SH, Seo JS, Yoon YJ, Kim KW, Yoo SY, Kim HS, Kook JK, Lee BR, Cha JH, Park JY. Effect of leaf-extract from Camellia sinensis and seed-extract from Casia tora on viability of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces. Korea J Orthod. 2003;33:381-389.
19 Petti S, Scully C. Polyphenols, oral health and disease: A review. J Dent. 2009;37:413-423.   DOI   ScienceOn
20 Liu J. Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol. 1995;49:57-68.   DOI   ScienceOn
21 Kim MJ, Kim CS, Ha WH, Kim BH, Lim YK, Park SN, Cho YJ, Kim M, Ko JH, Kwon SS, Ko YM, Kook JK. Antimicrobial effects of oleanolic acid against Streptococcus mutans and Streptococcus sobrinus isolated from a Korean population. Int J Oral Biol. 2010; 35:191-195.
22 Horiuchi K, ShiotaS, Hatano T, Yoshida T, Kuroda T, Tsuchiya T. Antimicrobial activity of oleanolic acid from Salvia officinalis and related compounds on vancomycinresistant enterococci (VRE). Biol Pharm Bull. 2007; 30:1147-1149.   DOI   ScienceOn
23 Fontanay S, Grare M, Mayer J, Finance C, Duval RE. Ursolic, oleanolic and betulinic acids: antibacterial spectra and selectivity indexes. J Ethnopharmacol. 2008; 120:272-276.   DOI   ScienceOn
24 Han C, Guo J. Antibacterial and anti-inflammatory activity of traditional Chinese herb pairs, Angelica sinensis and Sophora flavescens. Inflammation. 2012;35:913-919.   DOI   ScienceOn
25 Kim CS, Park SN, Ahn SJ, Seo YW, Lee YJ, Lim YK, Freire MO, Cho E, Kook JK. Antimicrobial effect of sophoraflavanone G isolated from Sophora flavescens against mutans streptococci. Anaerobe 2013;19:17-21.   DOI   ScienceOn
26 National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 5th ed. Approved standard M7-A5. Wayne, Pa, USA: NCCLS; 2000.
27 Cha JD, Jeong MR, Jeong SI, Lee KY. Antibacterial activity of sophoraflavanone G isolated from the roots of Sophora flavescens. J Microbiol Biotechnol. 2007;17:858-864.
28 Neal RG, Craig RG, Powers JM. Effect of sterilization and irrigants on the cutting ability of stainless steel files. J Endod. 1983;9:93-96.   DOI   ScienceOn
29 Tredwin CJ, Scully C, Bagan-Sebastian JV. Drug-induced disorders of teeth. J Dent Res. 2005;84:596-602.   DOI   ScienceOn
30 Gentil M, Pereira JV, Sousa YT, Pietro R, Neto MD, Vansan LP, de Castro Franva S. In vitro evaluation of the antibacterial activity of Arctium lappa as a phytotherapeutic agent used in intracanal dressings. Phytother Res. 2006;20:184-186.   DOI
31 Park SN, Kook JK. Antimicrobial activity of oleanolic acid, ursolic acid, and sophoraflavanone G against periodontopathogens. Int J Oral Biol. 2013;38:149-154.   DOI
32 Hichri F, Jannet JC, Cheriaa J, Jegham S, Mighri Z. Antibacterial activities of a few prepared derivatives of oleanolic acid and of other natural triterpenic compounds. C R Chimie. 2003;6:473-483.   DOI   ScienceOn
33 Zehnder M. Root canal irrigants. J Endod. 2006; 32:389-498.   DOI   ScienceOn
34 Yesilsoy C, Whitaker E, Cleveland D, Phillips E, Trope M. Antimicrobial and toxic effects of established and potential root canal irrigants. J Endod. 1995;21:513-515.   DOI   ScienceOn
35 Rocas IN, Siqueira JF Jr, Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;30:315-320.   DOI   ScienceOn
36 Kim MJ, Kim CS, Park JY, Lim YK, Park SN, Ahn SJ, Jin DC, Kim TH, Kook JK. Antimicrobial effects of ursolic acid against mutans streptococci isolated from Koreans. Int J Oral Biol. 2011;36:7-11.