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http://dx.doi.org/10.11620/IJOB.2013.38.2.043

Development of Streptococcus sanguinis-, Streptococcus parasanguinis-, and Streptococcus gordonii-PCR Primers Based on the Nucleotide Sequences of Species-specific DNA Probes Screened by Inverted Dot Blot Hybridization  

Park, Soon-Nang (Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University)
Kook, Joong-Ki (Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University)
Publication Information
International Journal of Oral Biology / v.38, no.2, 2013 , pp. 43-49 More about this Journal
Abstract
The objective of this study was to develop PCR primers that are specific for Streptococcus sanguinis, Streptococcus parasanguinis, and Streptococcus gordonii. We designed the S. sanguinis-, S. parasanguinis-, and S. gordonii-specific primers, Ssa21-F3/Ssa21-R2, Spa17-F/Spa17-R, and Sgo41-F1/Sgo41-R1 respectively, based on the nucleotide sequences of the Ssa21, Spa17, and Sgo41 DNA probes that were screened using inverted dot blot hybridization (IDBH). The species-specificity of these primers was assessed against 43 strains of mitis group streptococci, including clinical strains of S. sanguinis, S. parasanguinis, and S. gordonii. The resulting PCR data revealed that species-specific amplicons had been obtained from all strains of the target species tested, and that none of these amplicons occurred in any other strains from other species. These results suggest that the Ssa21-F3/Ssa21-R2, Spa17-F/Spa17-R, and Sgo41-F1/Sgo41-R1 primers may be useful in detecting S. sanguinis, S. parasanguinis, and S. gordonii at the species level, respectively.
Keywords
Streptococcus gordonii; Streptococcus sanguinis; Streptococcus parasanguinis; species-specific PCR primer; IDBH;
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