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Global Transcriptional Analysis Reveals Upregulation of NF-${\kappa}B$-responsive and Interferon-stimulated Genes in Monocytes by Treponema lecithinolyticum Major Surface Protein  

Lee, Sung-Hoon (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University)
Lee, Hae-Ri (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University)
Jun, Hye-Kyoung (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University)
Choi, Bong-Kyu (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University)
Publication Information
International Journal of Oral Biology / v.36, no.2, 2011 , pp. 91-101 More about this Journal
Abstract
MspTL is the major surface protein of Treponema lecithinolyticum associated with periodontitis and endodontic infections. Our recent investigation revealed that MspTL induces proinflammatory cytokines and intercellular adhesion molecule 1 in THP-1 cells and periodontal ligament cells. In this study we conducted oligonucleotide microarray analysis to investigate the global transcriptional regulation in THP-1 cells stimulated with purified recombinant MspTL. MspTL upregulated the expression of 90 genes in THP-1 cells at least four fold, and the functions of these genes were categorized into adhesion, apoptosis/antiapoptosis, cell cycle/growth/differentiation, chemotaxis, cytoskeleton organization, immune response, molecular metabolism, proteolysis, signaling, and transcription. The majority of the modified genes are known to be NF-${\kappa}B$-responsive and interferon-stimulated genes (ISGs). The expression of 12 selected genes was confirmed by real-time RT-PCR. Because prostaglandin $E_2(PGE_2)$ is an important inflammatory mediator and Cox-2 was found to be induced by MspTL in the microarray analysis, we determined the level of $PGE_2$ in the culture supernatants of MspTL-treated cells and found that MspTL significantly increased $PGE_2$. Our results provide insight into the gene regulation of host cells in response to MspTL, and may contribute to the understanding of the molecular mechanism in periodontitis.
Keywords
T. lecithinolyticum; Surface protein; Oligonulceotide microarray;
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1 Choi BK, Jung JH, Suh HY, Yoo YJ, Cho KS, Chai JK, Kim CK. Activation of matrix metalloproteinase-2 by a novel oral spirochetal species Treponema lecithinolyticum. J Periodontol. 2001a;72:1594-600.   DOI   ScienceOn
2 Choi EK, Kang IC. Production of pro-inflammatory cytokines by Porphyromonas gingivalis in THP-1 macrophagic cells. Int J Oral Biol. 2009;34:87-95
3 Choi BK, Moon SY, Cha JH, Kim KW, Yoo YJ. Prostaglandin $E_{2}$ is a main mediator in receptor activator of nuclear factorkappaB ligand-dependent osteoclastogenesis induced by Porphyromonas gingivalis, Treponema denticola, and Treponema socranskii. J Periodontol. 2005;76:813-20.   DOI   ScienceOn
4 Choi BK, Ohk SH, Lee HJ, Kang JH, Jeong GJ, Yoo YJ. Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation. J Periodontol 2001b;72: 1172-77.   DOI   ScienceOn
5 Voyich JM, Braughton KR, Sturdevant DE, Whitney AR, Saïd- Salim B, Porcella SF, Long RD, Dorward DW, Gardner DJ, Kreiswirth BN, Musser JM, DeLeo FR. Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils. J Immunol. 2005;175:3907-19.   DOI
6 Walter C, Zahlten J, Schmeck B, Schaudinn C, Hippenstiel S, Frisch E, Hocke AC, Pischon N, Kuramitsu HK, Bernimoulin JP, Suttorp N, Krüll M. Porphyromonas gingivalis straindependent activation of human endothelial cells. Infect Immun. 2004;72:5910-18.   DOI   ScienceOn
7 Wyss C, Choi BK, Schupbach P, Moter A, Guggenheim B, Göbel UB. Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases. Int J Syst Bacteriol 1999;49:1329-39.   DOI   ScienceOn
8 Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity. 2000;12:121-7.   DOI   ScienceOn
9 Lee SH, Kim KK, Rhyu IC, Koh S, Lee DS, Choi BK. Phenolwater extract of Treponema socranskii subsp. socranskii as an antagonist of Toll-like receptor 4 signaling. Microbiology. 2006;152:535-46.
10 Lee SH, Kim KK, Choi BK. Upregulation of intercellular adhesion molecule 1 and proinflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections. Infect Immun. 2005;73:268-76.   DOI   ScienceOn
11 Leong ML, Maiyar AC, Kim B, O'Keeffe BA Firestone GL. Expression of the serum- and glucocorticoid-inducible protein kinase, Sgk, is a cell survival response to multiple types of environmental stress stimuli in mammary epithelial cells. J Biol Chem 2003; 278:5871-82.   DOI
12 Nau GJ, Richmond JF, Schlesinger A, Jennings EG, Lander ES, Young RA. Human macrophage activation programs induced by bacterial pathogens. Proc Natl Acad Sci USA. 2002; 99:1503-8.   DOI   ScienceOn
13 Siqueira JF Jr, Rocas IN. PCR-based identification of Treponema maltophilum, T. amylovorum, T. medium, and T. lecithinolyticum in primary root canal infections. Arch Oral Biol. 2003;48:495-502.   DOI   ScienceOn
14 Nomura F, Kawai T, Nakanishi K, Akira S. NF-kappaB activation through IKK-i-dependent I-TRAF/TANK phosphorylation. Genes Cells. 2000;5:191-202.   DOI   ScienceOn
15 Ren Q, Robertson SJ, Howe D, Barrows LF, Heinzen RA. Comparative DNA microarray analysis of host cell transcriptional responses to infection by Coxiella burnetii or Chlamydia trachomatis. Ann N Y Acad Sci. 2003;990:701-13.   DOI   ScienceOn
16 Sankar S, Chan H, Romanow WJ, Li J, Bates RJ. IKK-i signals through IRF3 and NFkappaB to mediate the production of inflammatory cytokines. Cell Signal. 2006;18:982-93   DOI   ScienceOn
17 Ishii K, Kurita-Taniguchi M, Aoki M, Kimura T, Kashiwazaki Y, Matsumoto M, Seya T. Gene-inducing program of human dendritic cells in response to BCG cell-wall skeleton (CWS), which reflects adjuvancy required for tumor immunotherapy. Immunol Lett. 2005;98:280-90.   DOI   ScienceOn
18 Kuramitsu HK, Kang IC Qi M. Interactions of Porphyromonas gingivalis with host cells: implications for cardiovascular diseases. J Periodontol. 2003;74:85-9.   DOI   ScienceOn
19 Jin HK, Lee YH, Jeong SY, Na HS, Park HR, Chung J. Induction of signal transduction pathway genes in dendritic cells by lipopolysaccharides from Porphyromonas gingivalis and Escherichia coli. Int J Oral Biol. 2010;35:113-9
20 Kabashima H, Yoneda M, Nagata K, Hirofuji T, Maeda K. The presence of chemokine (MCP-1, MIP-1alpha, MIP-1beta, IP-10, RANTES)-positive cells and chemokine receptor (CCR5, CXCR3)-positive cells in inflamed human gingival tissues. Cytokine. 2002;20:70-7.   DOI   ScienceOn
21 Hemmi H, Takeuchi O, Sato S, Yamamoto M, Kaisho T, Sanjo H, Kawai T, Hoshino K, Takeda K, Akira S. The roles of two IkappaB kinase-related kinases in lipopolysaccharide and double stranded RNA signaling and viral infection. J Exp Med. 2004;199:1641-50.   DOI   ScienceOn
22 Cohen P, Bouaboula M, Bellis M, Baron V, Jbilo O, Poinot- Chazel C, Galiègue S, Hadibi EH, Casellas P. Monitoring cellular responses to Listeria monocytogenes with oligonucleotide arrays. J Biol Chem. 2000; 275:11181-90.   DOI
23 de Veer MJ, Holko M, Frevel M, Walker E, Der S, Paranjape JM, Silverman RH, Williams BR. Functional classification of interferon-stimulated genes identified using microarrays. J Leukoc Biol. 2001;69:912-20.
24 Emingil G, Atilla G, Huseyinov A. Gingival crevicular fluid monocyte chemoattractant protein-1 and RANTES levels in patients with generalized aggressive periodontitis. J Clin Periodontol. 2004:31:829-34.   DOI   ScienceOn
25 Fujigaki S, Saito K, Sekikawa K, Tone S, Takikawa O, Fujii H, Wada H, Noma A, Seishima M. Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-gamma-independent mechanism. Eur J Immunol. 2001; 31:2313-18.   DOI   ScienceOn
26 Galindo CL, Fadl AA, Sha J, Pillai L, Jr Gutierrez C, Chopra AK. Microarray and proteomic analyses of human intestinal epithelial cells treated with the Aeromonas hydrophila cytotoxic enterotoxin. Infect Immun. 2005;73:2628-43.   DOI   ScienceOn
27 Barnes M, Freudenberg J, Thompson S, Aronow B, Pavlidis P. Experimental comparison and cross-validation of the Affymetrix and Illumina gene expression analysis platforms. Nucleic Acids Res. 2005;33:5914-23.   DOI   ScienceOn