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Effects of Chitosan on the Differentiation of MDPC-23 Cells  

Park, Ju-Hyun (Oral Biology Research Institute, Chosun University School of Dentistry)
Kim, Do-Kyung (Oral Biology Research Institute, Chosun University School of Dentistry)
Park, Jong-Tae (Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 Project, Yonsei University College of Dentistry)
Kim, Su-Young (Oral Biology Research Institute, Chosun University School of Dentistry)
Yu, Sun-Kyoung (Oral Biology Research Institute, Chosun University School of Dentistry)
Cho, Kwang-Hee (Oral Biology Research Institute, Chosun University School of Dentistry)
Kim, Heung-Joong (Oral Biology Research Institute, Chosun University School of Dentistry)
Publication Information
International Journal of Oral Biology / v.35, no.3, 2010 , pp. 91-97 More about this Journal
Abstract
The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.
Keywords
chitosan; odontoblast; differentiation;
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Times Cited By KSCI : 2  (Citation Analysis)
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