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Osteogenic Differentiation Potential in Parthenogenetic Murine Embryonic Stem Cells  

Kang, Ho-In (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry)
Cha, Eun-Sook (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry)
Choi, Young-Ju (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry)
Min, Byung-Moo (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry)
Roh, Sang-Ho (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry)
Publication Information
International Journal of Oral Biology / v.33, no.3, 2008 , pp. 91-95 More about this Journal
Abstract
Embryonic stem cells have a pluripotency and a potential to differentiate to all type of cells. In our previous study, we have shown that embryonic stem cells (ESCs) lines can be generated from murine parthenogenetic embryos. This parthenogenetic ESCs line can be a useful stem cell source for tissue repair and regeneration. The defect in full-term development of parthenogenetic ESCs line enables researchers to avoid the ethical concerns related with ESCs research. In this study, we presented the results demonstrating that parthenogenetic ESCs can be induced into osteogenic cells by supplementing culture media with ascorbic acid and $\beta$-glycerophosphate. These cells showed morphologies of osteogenic cells and it was proven by Von Kossa staining and Alizarin Red staining. Expression of marker genes for osteogenic cells (osteopontin, osteonectin, alkaline phosphatase, osteocalcin, bone-sialoprotein, collagen type1, and Cbfa1) also confirmed osteogenic potential of these cells. These results demonstrate that osteogenic cells can be generated from parthenogenetic ESCs in vitro.
Keywords
Embryonic stem cell; Parthenogenesis; Osteogenic cell;
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1 Bourne S, Polak JM, Hughes S, Buttery SD. Osteogenic differentiation of mouse embryonic stem cells: Differential gene expression analysis by cDNA microarray and purification of osteoblasts by Cadherin-11 magnetically activated cell sorting. Tissue Eng. 2004;10:796-806   DOI   ScienceOn
2 Buttery LD., Bourne S, Xynos JD, Wood H, Hughes FJ, Hughes S, Episkopou,V, Polak, JM. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng. 2001;7:89-99   DOI   ScienceOn
3 Caplan AI. Tissue engineering designs for the future: new logics, old molecules. Tissue Eng. 2000;6:1-8   DOI   ScienceOn
4 Cummine J, Armstrong L, Nade S. Osteogenesis after bone and bone marrow transplantation. Acta. Orthop. Scand. 1983;54:235- 241   DOI   ScienceOn
5 Damien JC, Parson JR. Bone graft and bone graft substitutes: a review of current technology and applications. J. Appl. Biomater. 1991;2:187-208   DOI   ScienceOn
6 D'Ippolito G, Schiller PC, Ricordi C, Roos B, Howard GA. Agerelated osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J. Bone Miner. Res. 1999;14:1115-1122   DOI   ScienceOn
7 Freymana TM, Yannasb IV, Gibson LJ. Cellular materials as porous scaffolds for tissue engineering. Prog. in Mat. Sci. 2001;46: 273-282   DOI   ScienceOn
8 Hujaa SS, Robertsb WE. Mechanism of osseointegration - characterization of supporting bone with indentation testing and backscattered imaging. Seminars in Ortho. 2004; 10:162-173   DOI   ScienceOn
9 Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified culture expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 1997;64: 295-312   DOI   ScienceOn
10 Kim K, Lerou P, Yabuuchi A, Lengerke C, Ng K, West J, Kirby A, Daly MJ, Daley GQ. Differentiation potential of histocompatible parthenogenetic embryonic stem cells. Science. 2007; 315:482-486   DOI   ScienceOn
11 Nagy A, Gertsenstein M, Vintersten K, Behringer R. Manipulating the mouse embryo: A laboratory manual 3rd Edition, Cold Spring Harbor Laboratory Press, 2003
12 Quarto R, Thomas D, Liang CT. Bone progenitor cell deficit and age-associated decline in bone repair capacity. Calcif. Tissue Int. 1995;56:123-129   DOI
13 Lengerke C, Kim K, Lerou P, Daley GQ. Differentiation potential of histocompatible parthenogenetic embryonic stem cells. Ann. NY. Acad. Sci. 2007;1106:209-218   DOI   ScienceOn
14 Szmukler-Moncler S, Piattelli A, Favero GA, Dubruille JH. Considerations preliminary to the application of early and immediate loading protocols in dental implantology. Clin. Oral Implants Res. 2000;11: 12-25   DOI   ScienceOn
15 Zur Nieden NI, Price FD, Davis LA, Everitt RE, Rancourt DE. Gene profiling on mixed embryonic stem cell populations reveals a biphasic role for beta-catenin in osteogenic differentiation. Mol. Endocrinol. 2007;21: 674-685   DOI   ScienceOn
16 Bielby RC, Boccaccini AR, Polak JM, Buttery LD. In vitro Differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells. Tissue Eng. 2004;10:1518-1525   DOI
17 Bradley JA, Bolton EM, Pedersen RA. Stem cell medicine encounters the immune system . Nat. Rev. Immunol. 2002; 2:859-871   DOI   ScienceOn
18 Crane GM, Ihauc SL, Mikos AG. Bone tissue engineering. Nat. Med. 1995;1:1322-1324   DOI
19 Ohgushi H, Goldberg VM, Caplan AI. Heterotopic osteo-genesis in porous ceramics induced by marrow cells. J.Orthop. Res. 1989;7:566-578
20 McCulloch CA, Strugurescu M, Hughes F, Melcher AH, Aubin JE. Osteogenic progenitor cells in rat bone marrow stromal ppopulations exhibit self-renewal in culture. 1991; Blood 77:1906-1911
21 Smith DE, Zarb GA. Criteria for success of osseointegrated endosseous implants. J. Prosthet Dent. 1989;62: 567-572   DOI   ScienceOn
22 Daar AS, Greenwood HL. A proposed definition of regenerative medicine. J. Tissue Eng. Regen. Med. Medicine. 2007;1:179- 184   DOI   ScienceOn
23 Langer R, Vacanti JP. Tissue engineering. Science. 1993; 260:920-926   DOI
24 Minuth WW, Sittinger M, Kloth S. Tissue engineering: generation of differentiated artificial tissue for biomedical applications. Cell Tissue Res. 1998;291:1-9   DOI
25 Nanci A. Ten Cate's Oral Histology: Development, Structure, and Function, Vol.7, pp 345-348, Mosby Press, St. Louis, 2003
26 Caplan AI, Bruder SP. Cell and molecular engineering of bone regeneration, pp 603-618, Academic Press, 1997
27 Choi YJ, Won C, Kang H, Min BM, Roh S. Establishment of ES cell lines derived from mouse parthenogenetic embryo. Reprod. Dev. Biol. 2006;30 (suppl 2):122