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The effect of combined Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max (OPB) extracts on bone mineral density in ovariectomized rats.  

Kim, Jung-Keun (Dept. of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Kim, Se-Won (Dept. of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Ko, Seon-Yle (Dept. of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Kim, Se-Nyun (Dept. of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Kwon, Jong-Seok (Dept. of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Hwang, Hyeon-Hwan (Dept. of Safety and Toxicology, Research Institute, Oscotec. Inc.)
Publication Information
International Journal of Oral Biology / v.32, no.4, 2007 , pp. 143-151 More about this Journal
Abstract
This study was conducted to investigate the preventing effects of OPB (Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max extracts) and combined OPB/Calcium therapy on bone loss in ovariectomized rats. Sixty Sprague Dawley rats of 12-week-old were divided into eight groups: OVX (ovariectomized), OPBL (OPB 50 mg/kg), OPBM (OPB100 mg/kg), OPBH (OPB 200 mg/kg), OPBL/CAL(OPBL+CAL), OPBM/CAL (OPBM+CAL), OPBH/CAL (OPBH+CAL) and CAL (Calcium citrate 88.33 mg/kg+1$\alpha$, 25-dihydroxy-vitamin $D_3$ 33.33 IU/kg). Bone mineral density (BMD), bone mineral content (BMC), bone strength indices and cortical thickness were analyzed by peripheral quantitative computerized tomography (pQCT). pQCT scanning showed that OVX induced a significant decrease in trabecular bone mineral density and bone mineral content in the proximal tibia $(-36.4\pm2.4%,\;-21.8\pm12.7%)$. These decreases were significantly prevented by the administration of OPBM and OPBM/CAL. Cortical BMD and BMC of tibia were slightly enhanced by OPB and OPB/CAL. However there was no significant difference between OVX and OPB, OPB/CAL treated group. Bone strength indices and cortical thickness were not significantly different. Our results suggest that OPB and combined OPB/Calcium therapy are effective in preventing the development of bone loss induced by ovariectomy in rats.
Keywords
Rehmannia glutinosa Libosch; Eleutherococcus senticosus Max; Osteoporosis; bone mineral density; bone mineral content;
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