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Molecular Characterization of $Ca_v2.3$ in Rat Trigeminal Ganglion Neurons  

Fang, Zhi (Department of Physiology and Program in Molecular and Cellular Neuroscience, College of Dentistry and Dental Research Institute, Seoul National University)
Kim, Joong-Soo (Department of Physiology and Program in Molecular and Cellular Neuroscience, College of Dentistry and Dental Research Institute, Seoul National University)
Oh, Seog-Bae (Department of Physiology and Program in Molecular and Cellular Neuroscience, College of Dentistry and Dental Research Institute, Seoul National University)
Publication Information
International Journal of Oral Biology / v.31, no.2, 2006 , pp. 45-51 More about this Journal
Abstract
R-type($Ca_v2.3$) calcium channel contributes to pain sensation in peripheral sensory neurons. Six isoforms of $Ca_v2.3$ that result from combinations of presence or deletion of three inserts(insert I and insert in the II-III loop, and insert III in N-terminal regions) have been demonstrated to be present in different mammalian tissues. However, the molecular basis of $Ca_v2.3$ in trigeminal ganglion(TG) neurons is not known. In the present study, we determined which isoforms of $Ca_v2.3$ are expressed in rat TG neurons using the RT-PCR analysis. Whole tissue RT-PCR analyses revealed that only two isoforms, $Ca_v2.3a$ and $Ca_v2.3e$, were present in TG neurons. From single-cell RT-PCR, we found that $Ca_v2.3e$ rather than $Ca_v2.3a$ was the major isoform expressed in TG neurons, and $Ca_v2.3e$ was preferentially detected in small-sized neurons that express nociceptive marker, transient receptor potential vanilloid 1(TRPV1). Our results suggest that $Ca_v2.3e$ in trigeminal neurons may be a potential target for the pain treatment.
Keywords
$Ca_v2.3$; voltage-activated calcium channels; trigeminal ganglion; nociceptor;
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