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Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared t6 Benign Oral Epithelial Lesions  

Son, Hyun-Jin (Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Chu, Jung-Youb (Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Cho, Eui-Sic (Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Lee, Dong-Geun (Department of Pathology, School of Medicine, Chonbuk National University)
Min, Myung-Gee (Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Lee, Suk-Keun (Department of Oral Pathology, College of Dentistry, Kangnung National University)
Cho, Nam-Pyo (Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
Publication Information
International Journal of Oral Biology / v.31, no.2, 2006 , pp. 27-32 More about this Journal
Abstract
Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline mutations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast cancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma(SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign epithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.
Keywords
CDH1; E-Cadherin; CpG Methylation; Oral Squamous Cell Carcinoma;
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