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http://dx.doi.org/10.5125/jkaoms.2011.37.3.161

Expression of Bcl-2 in the epithelial lining and clinical findings of keratocystic odotogenic tumor  

Lee, In-Hyuk (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University)
Choi, So-Young (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University)
Park, Ji-Hoon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University)
Kim, Chin-Soo (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.37, no.3, 2011 , pp. 161-168 More about this Journal
Abstract
Purpose: The odontogenic keratocysts demonstrated a high recurrence rate and a biologically aggressive nature. This might be due to unknown factors inherent in the epithelium or enzymatic activity in the fibrous wall. Bcl-2 protein is characterized by its ability to inhibit apoptosis. The aim of this study was to evaluate the expression and distribution of bcl-2 in the OKCs, its possible relationship with the tumorous characteristics, such as the aggressive nature and high recurrence rate, and its usefulness to differentiate OKCs from dentigerous cysts. Materials and Methods: Formalin fixed paraffin-embedded tissue sections of 53 OKCs, and 44 dentigerous cyst were immunohistochemically analyzed quantitatively for the immunoreactivity of the bcl-2 protein with i-solution. Results: More Bcl-2 expression was observed in the OKCs (mean34.387%) than dentigerous cyst (mean11.144%) with statistical significance (P<0.001). Seventeen and 15 of the 32 OKCs in this study showed positivity in the basal layer and basal/suprabasal layers, respectively. In dentigerous cyst, 2 of 3 showed positivity in the basal cell layer. Conclusion: Considering that bcl-2 over expression may lead to the increased survival of epithelial cells, this study demonstrated a possible relationship between the aggressive nature of OKC and the intrinsic growth potential of its lining epithelium. Furthermore, the basal/suprabasal distribution of bcl-2 positive cells was observed in some OKCs, which might have a significant impact on the behavior of cysts. The bcl-2 expression of OKCs can be useful for differentiating OKCs from dentigerous cysts.
Keywords
Bcl-2; Keratocystic Odontogenic Tumor; Dentigerous cyst;
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1 Ogden GR, Chisholm DM, Kiddie RA, Lane DP. P53 protein in odontogenic cysts: increased expression in some odontogenic keratocysts. J Clin Pathol 1992:45:1007-10.   DOI
2 Eid H, Gulyas M, Geczi L, Bodrogi I, Institoris E, Bak M. Expression of bcl-2 in testicular carcinoma: correlation with tumor progression and MDR1/Pgp. Cancer 1998;83:331-6.   DOI   ScienceOn
3 Lu QL, Abel P, Foster CS, Lalani EN. Bcl-2: role in epithelial differentiation and oncogenesis. Hum Pathol 1996;27:102-10.   DOI   ScienceOn
4 Piattelli A, Fioroni M, Rubini C. Differentiation of odontogenic keratocysts from other odontogenic cysts by the expression of bcl-2 immunoreactivity. Oral Oncol 1998;34:404-7.   DOI   ScienceOn
5 Pezzella F, Turley H, Kuzu I, Tungekar MF, Dunnill MS, Pierce CB, Harris A, Gatter KC, Mason DY. BCL-2 protein in nonsmall- cell lung carcinoma. N Engle J Med 1993;329:690-4.   DOI   ScienceOn
6 Virkajarvi N, Paakko, Soini Y. Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma. Histopathology 1998;33:432-9.   DOI
7 Gee JM, Robertson JF, Ellis IO, Willsher P, McClelland RA, Hoyle HB, et al. Immunocytochemical localization of BCL-2 protein in human breast cancers and its relationship to a series of prognostic markers and response to endocrine therapy. Int J Cancer 1994;59:619-28.   DOI   ScienceOn
8 Colombel M, Symmans F, Gil S, O'Toole KM, Chopin D, Benson M, et al. Detection of the apoptosis-suppressing oncoprotein bcl-2 in hormone-refractory human prostate cancers. Am J Pathol 1993 Aug;143:390-400.
9 Lauwers GY, Scott GV, Hendricks J. Immunohistochemical evidence of aberrant bcl-2 protein expression in gastric epithelial dysplasia. Cancer 1994;73:2900-4.   DOI   ScienceOn
10 Skopelitou A, Hadjiyannakis M, Alexopoulou V, Krikoni O, Kamina S, Agnantis N. Topographical immunohistochemical expression of bcl-2 protein in human liver lesions. Anticancer Res 1996;16:975-8.
11 Song MY, Lee SS, Lee JK, Yi WJ, Heo MS, Lee JI, et al. Relation of the radiologic findings and labeling index of Ki-67, PCNA and cytokeratin in unicystic ameloblastoma, dentigerous cyst and odontogenic keratocyst. Korean J Oral Maxillofac Radiol 2004;34:75-9.
12 Stoll C, Stollenwerk C, Riediger D, Mittermayer C, Alfer J. Cytokeratin expression patterns for distinction of odontogenic keratocysts from dentigerous and radicular cysts. J Oral pathol Med 2005;34:558-64.   DOI   ScienceOn
13 Lee SG, Lee JH, Kim CH. Immunohistochemical Study of Odontogenic Keratocyst. J Korean Assoc Maxillofac Plast Reconstr Surg. 2004;26:469-76.
14 Slootweg PJ. P53 protein and Ki-67 reactivity in epithelial odontogenic lesions. An immunohistochemical study. J Oral Pathol Med 1995 Oct;24(9):393-7.   DOI   ScienceOn
15 Hockenbery DM, Zutter M. Hickey W, Nahm M, Korsmeyer SJ. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death. Proc Natl Acad Sci USA 1991; 88(16):6961-5.   DOI   ScienceOn
16 Meara JG, Pilch BZ, Shah SS, Cunningham MJ. Cytokeratin expression in the odontogenic keratocyst. J Oral Maxillofac Surg 2000;58:862-5.   DOI   ScienceOn
17 Rodriguez-Villanueva J, Colome MI, Brisbay S, McDonnell TJ. The expression and localization of bcl-2 protein in normal skin and in non-melanoma skin cancers. Pathol Res Pract 1995;191:391-8.   DOI   ScienceOn
18 Birchall MA, Winterford CM, Allan DJ, Harmon BV. Apoptosis in normal epithelium, premalignant and malignant lesions of the oropharynx and oral cavity: a preliminary study. Eur J Cancer B Oral Oncol 1995;31B(6):380-3.
19 August M, Faquin WC, Troulis M, Kaban LB. Differentiation of odontogenic keratocysts from nonkeratinizing cyst by use of fineneedle aspiration biopsy and cytokeratin-10 staining. J Oral Maxilloc Surg 2000;58:935-40.   DOI   ScienceOn
20 el-Hajj G, Anneroth G. Odontogenic keratocysts-a retrospective clinical and histologic study. Int J Oral Maxillofac Surg. 1996; 25:124-9.   DOI   ScienceOn
21 Singh BB, Chandler FW Jr, Whitaker SB, Forbes-Nelson AE. Immunohistochemical evaluation of bcl-2 oncoprotein in oral dysplasia and carcinoma. Oral Surg Oral Med Oral Pathol Oral Oadiol Endod 1998;85:692-8.   DOI   ScienceOn
22 Madras J, Lapointe H. Keratocystic Odontogenic Tumour: reclassification of the odontogenic keratocyst from cyst to tumour. J Can Dent Assoc. 2008;74:165-165h.
23 Chow HT. Odontogenic keratocyst: a clinical experience in Singapore. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;86:573-7.   DOI   ScienceOn
24 Chun SD, An CH, Choi KS. Radiographic study of the odontogenic keretocyst. Korean J Oral Maxillofac Radiol 2005;35:51-4.
25 Shear M. The aggressive nature of the odontogenic keratocyst: Is it a benign cystic neoplasm? Part 2. Proliferation and genetic studies. Oral Oncol 2002;38:323-31.   DOI   ScienceOn
26 Nicholson DW, Thornberry NA. Caspases: killer proteases. Trends Biochem Sci 1997;22:299-306.   DOI
27 Lo Muzio L, Staibano S, Pannone G, Bucci P, Nocini PF, Bucci E, De Rosa G. Expression of cell cycle and apoptosis-related proteins in sporadic odontogenic keratocysts and odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome. J Dent Res 1999;78:1345-53.   DOI   ScienceOn
28 Jahanshahi GH, Talebi A, Shirvani A. Expression of bcl-2 in the epithelial lining of odontogenic keratocysts. J Dent Tehran Univ Medical Sci, 2006;3:no 1
29 Singh B, Chandler F, Gaugham Jr G, Whitaker b, Zunt S. Immunohistochemical status of bcl-2 oncoprotein in the odontogenic keratocyst. In: Abstract and clinical pathologic conference cases presented at the 52nd Academy of Oral and Maxillofacial Pathology. Oral Surg Oral Med Oral Pathol 1998;86:207-19.   DOI   ScienceOn
30 Pindborg JJ, Hansen J. Studies on odontogenic cyst epithelium: 2.Clinical and roentgenologic aspects of odontogenic keratocysts. Acta Pathol Microbiol Scand 1963;58:283-94.
31 Giuliani M, Grossi GB, Lajolo C, Bisceglia M, Herb KE. Conservative management of a large odontogenic keratocyst: report of a case and review of the literature. J Oral Maxillofac Surg 2006;64:308-16.   DOI   ScienceOn
32 Barnes L. Surgical pathology of the head and neck. 1st ed. New York: Marcel Dekker; 1985.
33 Arafat A, Lunin M. Odontogenic keratocysts. J Baltimore Coll Dental Surg 1984;36:5-13.
34 Villa P, Kaufmann SH, Earnshaw WC. Caspases and caspase inhibitors. Trends Biochem Sci 1997;22:388-93.   DOI   ScienceOn
35 Kumar V, Abbas AK, Fausto N. Robbinson and Cotran pathologic basis of disease. 7th ed. Philadelpia: Elsevier Saunders; 2005: 26-32.
36 Philipsen HP. Om Keratocyster(Kolesteatomer) I Kaeberne. Tandlaegebladet 1956;60:963-80.
37 Perumalsamy LR, Nagala M, Sarin A. Notch-activated signaling cascade interacts with mitochondrial remodeling proteins to regulate cell survival. Proc Natl Acad Sci USA. 2010;107:6882-7.   DOI   ScienceOn
38 Kernohan NM, Cox LS. Regulation of apoptosis by bcl-2 and its related proteins: immunochemical challenges and therapeutic implications. J Pathol 1996;179:1-3.   DOI   ScienceOn
39 Donoff RB, Guralnick WC, Clayman L. Keratocysts of the jaws. J Oral Surg 1972;30:800-4.
40 Omura S, Kawabe R, Kobayashi S, Mizuki N. Odontogenic keratocyst appearing as a soap-bubble or honeycomb radiolucency: report of a case. J Oral Maxillofac Surg 1997;55:185-9.   DOI   ScienceOn
41 Philipsen HP. Keratocystic odontogenic tumour. In: Barnes L, Eveson JW, Reichart P, Sidransky D, eds. World Health Organization classification of tumours: pathology and genetics of head and neck tumours. Lyon: IARC Press; 2005:306-7.
42 Forssell K, Sorvari TE, Oksala E. A clinical and radiographic study of odontogenic keratocysts in jaws. Proc Finn Dent Soc 1974;70:121-34.
43 Marker P, Brondum N, Clausen PP, Bastian HL. Treatment of large odontogenic keratocysts by decompression and later cystectomy: a long-term follow-up and a histologic study of 23cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1996;82:122-131.   DOI   ScienceOn
44 Browne RM. The odontogenic keratocyst: histological features and their correlation with clinical behaviour. Br Dent J 1971; 131:249-59.   DOI   ScienceOn
45 Magnusson BC. Odontogenic keratocysts: a clicical and histological study with special reference to enzyme histochemistry. J Oral Pathol 1978;7:8-18.   DOI
46 Toller P. Origin and growth of cysts of the jaws. Ann R Coll Surg Engl 1967;40:306-36.
47 Shear M. Developmental odontogenic cysts: an update. J Oral Pathol Med 1994;23:1-11.   DOI   ScienceOn
48 Browne RM. The odontogenic keratocyst: clinical aspects. Br Dent J 1970;128:225-31.   DOI   ScienceOn
49 Ahlfors E, Larsson A, Sjogren S. The odontogenic keratocyst: a benign cystic tumor? J Oral Maxillofac Surg 1984;42:10-9.   DOI   ScienceOn
50 Brannon RB. The odontogenic keratocyst; a clinicopathologic study of 312 cases. Part I. Clinical features. Oral Surg Oral Med Oral Pathol 1976;42:54-72.   DOI   ScienceOn
51 Gonzalez-Alva P, Tanaka A, Oku Y, Yoshizawa D, Itoh S, Sakashita H, et al. Keratocystic odontogenic tumor: a retrospective study of 183 cases. J Oral Sci 2008;50:205-12.   DOI   ScienceOn