Browse > Article
http://dx.doi.org/10.5125/jkaoms.2010.36.1.1

Stromal cell-derived factor-1 (SDF-1) expression in the oral squamous cell carcinoma  

Kim, Kyung-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
Han, Se-Jin (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
Roh, Kyu-Seob (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.36, no.1, 2010 , pp. 1-6 More about this Journal
Abstract
Purpose: Chemokines are structurally related, small polypeptide signaling molecules that bind to and activate a family of transmembrane G protein-coupled receptors, the chemokine receptors. Recently, interaction between the chemokine receptor CXCR4 and its ligand, stromal cell-derived factor 1 (SDF-1 or CXCL12), has been found to play an important role in tumorigenicity, proliferation, metastasis and angiogenesis in many cancers such as lung cancer, breast cancer, melanoma, glioblastoma, pancreatic cancer and cholangiocarcinoma. Hence, the goal of this study is to identify the correlation of clinicopathological factors and the up-regulation of SDF-1 expression in oral squamous cell carcinoma. Material and methods: We studied the immunohistochemical staining of SDF-1, quantitative RT-PCR (qRT-PCR) of SDF-1 gene in 20 specimens of 20 patients with oral squamous cell carcinoma. Results: 1. In the immunohistochemical study of poor differentiated and invasive oral squamous cell carcinoma, the high level staining of SDF-1 was observed. And the correlation between immunohistochemical SDF-1 expression and tumor nodes metastases (TNM) classification of specimens was significant.($x^2$ test, P < 0.05) 2. In the SDF-1 gene qRT-PCR analysis, SDF-1 expression was more in tumor tissue than in carcinoma in situ tissue. Paired-samples analysis determined the difference of SDF-1 mRNA expression level between the cancer tissue and the carcinoma in situ tissue.(Student's t-test, P < 0.05) Conclusion: These findings suggest that up-regulation of the SDF-1 may play a role in progression and invasion of oral squamous cell carcinoma.
Keywords
Chemokine; Chemokine CXCL12 (SDF-1); Oral squamous cell carcinoma;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Scotton CJ, Wilson JL, Milliken D, Stamp G, Balkwill FR. Epithelial cancer cell migration: a role for chemokine receptors? Cancer Res 2001;61:4961-5.
2 Bertolini F, Dell'Agnola C, Mancuso P, Rabascio C, Burlini A, Monestiroli S, et al. CXCR4 neutralization, a novel therapeutic approach for non-Hodgkin's lymphoma. Cancer Res 2002;62:3106-12.
3 Hemmer J, Kreidler J. Flow cytometric DNA ploidy analysis of squamous cell carcinoma of the oral cavity. Comparison with clinical staging and histologic grading. Cancer 1990;66:317-20.   DOI   ScienceOn
4 Kim H, Muller WJ. The role of the epidermal growth factor receptor family in mammary tumorigenesis and metastasis. Exp Cell Res 1999;253:78-87.   DOI   ScienceOn
5 Rossi D. Zlotnik A. The biology of chemokines and their receptors. Annu Rev Immunol 2000;18:217-42.   DOI   ScienceOn
6 Parkin DM, Pisani P, Ferlay J. Estimates of the worldwide incidence of 25 major cancers in 1990. Int J Cancer 1999;80:827-41.   DOI   ScienceOn
7 Laing KJ, Secombes CJ. Chemokines. Dev Comp Immunol 2004;28:443-60.   DOI   ScienceOn
8 Murdoch C, Finn A. Chemokine receptors and the role in inflammation and infectious diseases. Blood 2000;95:3032-43.
9 Muller A, Homey B, Soto H, Ge N, Catron D, Buchanan M, et al. Involvement of chemokine receptors in breast cancer metastasis. Nature 2001;410:50-6.   DOI   ScienceOn
10 Kang H, Watkins G, Parr C, Douglas-Jones A, Mansel RE, Jiang WG. Stromal cell derived factor-1: its influence on invasiveness and migration of breast cancer cells in vitro, and its association with prognosis and survival in human breast cancer. Breast Cancer Research 2005;7:R402-10.   DOI
11 Mirshahi F. Pourtau J, Li H, Muraine M, Trochon V, Legrand E, et al. SDF-1 activity on microvascular endothelial cells: consequences on angiogenesis in in vitro and in vivo models. Thromb Res 2000;99:587-94.   DOI   ScienceOn
12 Molino M, Woolkalis MJ, Prevost N, Pratico′D, Barnathan ES, Taraboletti G, et al. CXCR4 on human endothelial cells can serve as both a mediator of biological responses and as a receptor for HIV-2. Biochim Biophys Acta 2000;1500:227-40.   DOI   ScienceOn
13 Parkin DM, Bray F, Ferlay J, Pisani P. Estimating the world cancer burden: Globocan 2000. Int J Cancer 2001;94:153-6.   DOI   ScienceOn
14 Datta SR, Brunet A, Greenberg ME. Cellular survival: a play in three Akts. Genes Dev 1999;13:2905-27.   DOI   ScienceOn
15 Evan GI, Vousden KH. Proliferation, cell cycle and apoptosis in cancer. Nature 2001;411:342-8.   DOI   ScienceOn
16 Dewan MZ, Ahmed S, Iwasaki Y, Ohba K, Toi M, Yamamoto N. Stromal cell-derived factor-1 and CXCR4 receptor interaction in tumor growth and metastasis of breast cancer. Biomed Pharmacother 2006;60:273-6.   DOI   ScienceOn
17 Hosokawa Y, Hosokawa I, Ozaki K, Nakae H, Murakami K, Miyake Y, et al. CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease. Clin Exp Immunol 2005;141:467-74.   DOI   ScienceOn
18 Fernandez EJ, Lolis E. Structure, function, and inhibition of chemokines. Annu Rev Pharmacol Toxicol 2002;42:469-99.   DOI   ScienceOn
19 Daly AJ, McIlreavey L, Irwin CR. Regulation of HGF and SDF-1 expression by oral fibroblasts-implications for invasion of oral cancer. Oral Oncol 2008;44:646-51.   DOI   ScienceOn
20 Sankaranarayanan R, Masuyer E, Swaminathan R, Ferlay J, Whelan S. .Head and neck cancer: a global perspective on epidemiology and prognosis. Anticancer Res 1998;18:4779-86.
21 Youngs SJ, Ali SA, Taub DD, Rees RC. Chemokines induce migrational responses in human breast carcinoma cell lines. Int J Cancer 1997;71:257-66.   DOI   ScienceOn
22 Taichman RS, Cooper C, Keller ET, Pienta KJ, Taichman NS, McCauley LK. Use of the stromal cell-derived factor-1/CXCR4 pathway in prostate cancer metastasis to bone. Cancer Res 2002;62:1832-7.
23 Carvalho AL, Magrin J, Kowalski LP. Sites of recurrence in oral and oropharyngeal cancers according to the treatment approach. Oral Dis 2003;9:112-8.   DOI   ScienceOn
24 Ghadjar P, Coupland SE, Na IK, Noutsias M, Letsch A, Stroux A,et al. Chemokine receptor CCR6 expression level and liver metastases in colorectal cancer. J Clin Oncol 2006;24:1910-6.   DOI   ScienceOn
25 Hill MW, Mackenzie IC. The influence of differing connective tissue substrates on the maintenance of adult stratified squamous epithelia. Cell Tissue Res 1984;237:473-8.
26 Uchida D, Begum NM, Almofti A, Nakashiro K, Kawamata H, Tateishi Y, et al. Possible role for stromal-cell-derived factor-1/CXCR4 signaling on lymph node metastasis of oral squamous cell carcinoma. Exp Cell Res 2003;290:289-302.   DOI   ScienceOn
27 Pisani P. Parkin DM, Bray F, Ferlay J. Estimates of the worldwide mortality from 25 cancers in 1990. Int J Cancer 1999;83:18-29.
28 Yang SX, Chen JH, Jiang XF, Wang QL, Chen ZQ, Zhao W, et al. Activation of chemokine receptor CXCR4 in malignant glioma cells promotes the production of vascular endothelial growth factor. Biochem Biophys Res Commun 2005;335:523-8.   DOI   ScienceOn
29 Strieter RM, Burdick MD, Mestas J, Gomperts B, Keane MP, Belperio JA. Cancer CXC chemokine networks and tumour angiogenesis. Eur J Cancer 2006;42:768-78.   DOI   ScienceOn
30 Murakami T, Maki W, Cardones AR, Fang H, Tun Kyi A, Nestle FO. Expression of CXC chemokine receptor-4 enhances the pulmonary metastatic potential of murine B16 melanoma cells. Cancer Res 2002;62: 7328-34.
31 Carvalho AL, Ikeda MK, Magrin J, Kowalski LP. Trends of oral and oropharyngeal cancer survival over five decades in 3267 patients treated in a single institution. Oral Oncol 2004;40:71-6.   DOI   ScienceOn