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AN ATOPIC NUDE MOUSE MODEL OF ORAL CANCER CELL LINE  

Kim, Jong-Hyun (Dept. of Oral & Maxillofacial surgery, College of Dentistry, Yonsei University)
Hwang, Young-Sun (Oral Cancer Institude, College of Dentistry, Yonsei University)
Kim, Hyun-Sil (Oral Cancer Institude, College of Dentistry, Yonsei University)
Nam, Woong (Dept. of Oral & Maxillofacial surgery, College of Dentistry, Yonsei University)
Cha, In-Ho (Dept. of Oral & Maxillofacial surgery, College of Dentistry, Yonsei University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.35, no.2, 2009 , pp. 74-82 More about this Journal
Abstract
In order to make successful oral cancer treatment, we need to understand about tumor biology and effective chemotherapeutic agents. To achieve these studies, it is necessary to develope a proper in-vivo model. Therefore the author will make try to develop more improved animal model of more applicable in various method of cancer study. In this study, the author induced in-vivo tumorigenesis in nude mice by $YD-10B_{mod}$ cell line used by YD-10B cell line originated from oral tongue squamous cell carcinoma and observed tumor formations and invasiveness of surrounding tissue, and found some results as follows : 1. The experimental group($YD-10B_{mod}$, subcutaneous injection) produced tumors 13 out of 15 mice, while the control group produced none of 5 mice. 2. The inoculation of $1{\times}10^6$cells/mouse produced tumors 3 out of 5 mice and inoculation of $1{\times}10^7$cells/mouse, $2{\times}10^7$cells/mouse produced tumors in every 5 mice. 3. In the histopathologic studies, the inoculation of $1{\times}10^6$cells/mouse group showed the characteristic features of well-differentiated squamous cell carcinoma and demarcated expansile growth, while the inoculation of $1{\times}10^7$cells/mouse, $2{\times}10^7$cells/mouse group showed the expansile growth with partial central necrosis and invasive growth to surrounding fat & connective tissue. These findings suggest that atopic xenograft of $YD-10B_{mod}$ cell line in nude mice has a improved productivity of tumors, produced tumors showed the characteristics feature of human tumor and invasive growth to surrounding tissue in histopathologic appearance. These atopic nude mouse model of tongue carcinoma might assist in studying oral cancer biology and effective choice of chemotherapeutic agents.
Keywords
Oral cancer; Tongue cancer; Squamous cell carcinoma; Atopic nude mouse model; Cell line;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Johnson NW : Global epidemiology in oral cancer. Shah JP, Johnson NW, Batsaki JG(eds), 2003; pp.10-20, Martin Dunitz, London
2 Hawkins BL, Heniford BW, Ackermann DM, Leonberger M, Martinez SA, Hendler FJ : 4NQO carcinogenesis : A mouse model of oral cavity squamous cell carcinoma. Head Neck 1994;16:424-432   DOI   PUBMED   ScienceOn
3 Rygaard J, Povlsen CO, : Heterotransplantation of a human malignant tumour to 'Nude' mice. Acta Pathol Microbiol Scand. 1969;77(4):758-760   DOI   PUBMED   ScienceOn
4 Hendrickson EA : The SCID mouse : relevance as an animal model system for studying human disease. Am J Pathol 1993;143:1511   ScienceOn
5 Hoffman, RM : Orthotopic is orthodox : why are orthotopictransplant metastatic models different from all other models? J. Cell. Biochem 1994;56:1-3   DOI   PUBMED   ScienceOn
6 Jeffrey N, Myers JN, Holsinger FC, Samar A, Jasser SA, Bekele BN, Fidler IJ : An orthotopic nude mouse model of oral tonguesquamous cell carcinoma. Clin Cancer Res 2002;8(1):293-8   PUBMED   ScienceOn
7 Holsinger FC, Doan DD, Jasser SA, Swan EA, Greenberg JS, Schiff BA : Epidermal growth factor receptor blockade potentiates apoptosis mediated by paclitaxel and leads to prolonged survival in an murine model of oral cancer. Clin Cancer Res 2003;9:3183-3189   PUBMED   ScienceOn
8 Bruns CJ, Garbison MT, Kuniyasu H, Eue I, Fidler IJ : In vivo selection and characterization of metastatic varients from human pancreatic adenocarcinoma by using orthotopic implantation in nude mice. Neoplasia 1999;1:50-62   DOI   PUBMED   ScienceOn
9 Lensch R, Gotz C, Andres C, Bex A, Lehmann J, Zwergel T : Comprehensive genotype analysis of human prostate cancer cell lines and sublines derived from metastases after orthotopic implantation in nude mice. Int J Oncol 2002;21:695-706   PUBMED   ScienceOn
10 Boeheim K, Teicher B, Ervin TJ, Shklar G : The effect of chemotherapeutic agents on human oral squamous cell carcinoma transplanted to nude mice: a histologic study. Oral Surg Oral Med Oral Pathol 1986;62(1):50-56   DOI   PUBMED   ScienceOn
11 박형국, 김용각 : Making in vivo model to study about human oral cancer. 대한악안면성형재건외과학회지 1997;19(3):300-310   과학기술학회마을
12 Huang J, Liang ZO, Wu TT, Hoque A, Chen H, Jiang Y, Zhang H, Xu XC : Tumor suppressive effect of retinoid-induced gene- 1(RRIG 1) in esophageal cancer. Cancer Res 2007;15,67(4):1589-93   DOI   PUBMED   ScienceOn
13 Kubota T : Metastastic models of human cancer xenografted in mice: the importance of orthotopic transplantation. J Cell Biochem 1994;56:4   DOI   ScienceOn
14 Huang SM, Li J, Armstrong EA, Harari PM : Modulation of radiation response and tumor-induced angiogenesis after epidermal growth factor receptor inhibition by ZD1839(Iressa). Cancer Res 2002;62(15):4300-6   PUBMED   ScienceOn
15 Dinesman A, Haughey B, Gates GA, Aufdemorte T, Von Hoff DD : Development of a new in vivo model for head and neck cancer. Otolaryngol Head Neck Surg 1990;103:766-774   DOI   PUBMED   ScienceOn
16 Umeda M, Komatsubara H, Nishimatsu N, Oku N, Shibuya Y, Yokoo S, et al : Establishment and characterization of a human adenoid cystic carcinoma line of the salivary gland which is serially transplantable and spontaneously metastasis to the lung in nude mice. Oral Oncol. 2002;38:30-34   DOI   PUBMED   ScienceOn
17 Myers JN, Holsinger FC, Jasser SA, Bekele BN, Fidler IJ : An orthotopic nude mouse model of oral squamous cell carcinoma. Clin Cancer Res 2002;8:293-298   PUBMED   ScienceOn
18 Takashi S, Masahiro Umeda, Hideki Komatsubara, et al : Lymph node and pulmonary metastasis after transplantation of oral squamous cell carcinoma cell line (HSC-3) into the subcutaneous tissue of nude mouse : detection of metastases by genetic methods using $\beta$-globin and mutant p53 genes. Oral Surg Oral Pathol Oral Radiol Endod 2008;105:486-90   DOI   PUBMED   ScienceOn
19 김수곤, 명훈, 김명진 : 누드마우스에 이종이식된 구강편평상 피세포암종에 대한 thalidomide의 항암효과와 혈관형성억제 에 관한 연구. 대한구강악안면외과학회지 2001;27(4):330-336   과학기술학회마을
20 Fidler IJ : Rationale and methods for the use of nude mice to study the biology and therapy of human cancer metastasis. Cancer Metastasis Rev 1986;5: 29-49   DOI   ScienceOn
21 명훈, 이종호, 정필훈, 김명진 : 구강편평세포암종 이식 누드 마우스에서 혈액점도 변화. 대한 구강악안면외과학회지 2004;30(2):81-84   과학기술학회마을
22 장명진, 이재훈 : 누드마우스에서 이종이식된 구강편평상피 세포암종에 대한 'ABNOBAviscum' 의 치료효과에 관한 실험적 연구. 대한악안면성형재건외과학회지 2003;25(5):383-397
23 Carew JF, Singh B, Shah JP : Factors affecting choice of treatment. In Oral Cancer. Shah JP, Johnson NW, Batsaki JG(eds), 2003; pp.209-213, Martin Dunitz, London
24 Giovanella BC, Stehiin JS : Heterotransplantation of human malignant tumors in "nude" thymusless mice. I. Breeding and maintenance of "nude" mice. J Natl Cancer Inst. 1973;51:615-619   PUBMED
25 박형욱, 정성훈 : 마우스에서 타액선암 동위종양 모델 제작을 위한 실험적 연구. 대한구강악안면외과학회지 2007;33:81- 93   과학기술학회마을
26 Odukoya O, Shklar G : Two-phase carcinogenesis in hamster buccal pouch. Oral Surg 1982;54:547-552   DOI   PUBMED   ScienceOn
27 McDermott-Lancaster RD, Ito T, Kohaka K, Colston MJ : The nude mouse characteristics, breeding and husbandary. Int J Leprosy 1987;55:885-888   PUBMED
28 Giovanella BC, Stehiin JS, Williams LJ : Heterotransplantation of human malignant tumors in nude thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J Natl Cancer Inst. 1974;52:921-930   DOI   PUBMED
29 Fu XY, Theodorescu D, Kerbel RS, Hoffman RM : Extensive multi organ metastasis following orthotopic onplantation of histologically intact human bladder carcinoma tissue in nude mice. Int J Cancer. 1991;49:938   DOI   PUBMED
30 Eun Ju Lee, Jin Kim, Seoung Ae Lee, Eun Jung Kim, Yong Chan Chun, Mi Heon Ryu, and Jong In Yook : Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells. Experimental and molecular Medicine 2005;37(5):379-390   DOI   ScienceOn
31 Khanna C, Hunter K : Modeling metastasis in vivo. Carcinogenesis 2005;26(3):513-523   DOI   PUBMED   ScienceOn
32 김문기 : 누드마우스에서 Snail 발현을 이용한 이종이식 종양의 침윤성 성장 유도. 박사학위 논문, 연세대학교 대학원, 서울, 2005
33 Santis H, Shklar G, Chauncey HH : Histochemistry of experimentary induced leukoplakia and carcinoma of the hamster buccal pouch. Oral Surg. 1964;17:207-218   DOI   PUBMED   ScienceOn
34 Killion JJ, Radinsky R, and Fidler IJ, : Orthotopic models are necessary to predict therapy of transplantable tumors in mice. Cancer metastasis Rev 1998;17:279-284   DOI   PUBMED   ScienceOn
35 Scheper MA, Nikitakis NG, Chaisuparat R, Montaner S, Sauk JJ : Sulindac induces apoptosis and inhibits tumor growth in vivo in head and neck Squamous cell carcinoma. Neoplasia 2007;9(3):192-9   DOI   PUBMED
36 Kim S, Prak YW, Schiff BA, Doan DD, Yazici Y, Jasser SA, et al : An Orthotopic model of anaplastic thyroid carcinoma. Clin Cancer Res. 2005;11:1713-1721   DOI   PUBMED   ScienceOn
37 Dinney CP, Fishbeck R, Singh RK, Eve B, Pathak S, Brown N : Isolation and characterization of metastatic variants from human transitional cell carcinoma passaged by orthotopic implantation in athymic nude mice. J Urol 1995;154:1532-1538   DOI   PUBMED   ScienceOn
38 Huang SM, Harari PM : Modulation of radiation response after epidermal growth factor recept or blockade in squamous cell carcinomas : inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis. Clin Cancer Res 2000;6(6):2166-74   PUBMED   ScienceOn