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EFFECT OF DIFFERENT STERILIZATION METHODS ON THE SURFACE MORPHOLOGY OF PPDO-hybrid-PLGA NANOFIBER SCAFFOLD AND ATTACHMENTS OF PC12 CELL  

Lee, Ju-Hyon (Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery)
Min, Hyun-Gi (Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery)
Jung, Ju-Young (Ewha Womans University Graduate School of Clinical Dentistry Department of lmplant Dentistry)
Kang, Na-Ra (Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.34, no.6, 2008 , pp. 635-639 More about this Journal
Abstract
Objectives : the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated. Methods : Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; Control:Non-sterilized scaffold, Group I:scaffold sterilized with 70% Alcohol, Group II: scaffold sterilized with Ethylene Oxide at $65^{\circ}C$, and Group III: scaffold sterilized with Ethylene Oxide at $37^{\circ}C$. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control: sterilized with 70% Alcohol, Group I: sterilized with Ethylene Oxide at $65^{\circ}C$, and Group II: sterilized with Ethylene Oxide at $37^{\circ}C$. Results : 1. The surface morphology was slightly changed in Group I, II and Group III, compared with control. 2. The attachment of PC12 cells in Group I, II was not higher than in control Discussion : The attachment of PC12 cell is not influenced by different sterilization methods.
Keywords
Sterilization; Nanofiber scaffold; Surface morphology; Attachment of cell;
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