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THE EFFECTS OF ${\beta}-TCP$/rhBMP-2 ON BONE FORMATION IN OSTEOBLAST-LIKE CELLS INDUCED FROM BONE MARROW-DERIVED MESENCHYMAL STEM CELLS  

Choi, Yong-Soo (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University)
Hwang, Kyung-Gyun (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University)
Lee, Jae-Seon (Institute of Biomedical Science, College of Medicine, Hanyang University)
Park, Chang-Joo (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University)
Shim, Kwang-Sup (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.34, no.4, 2008 , pp. 419-427 More about this Journal
Abstract
The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on ${\beta}-tricalcium$ phosphate (${\beta}-TCP$) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM ${\beta}$-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor ${\kappa}B$ ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-Ⅰ (COL-Ⅰ). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on ${\beta}-TCP(+/-)$. According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/${\beta}-TCP(-)$ than DOCs/${\beta}-TCP(+)$. According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/${\beta}-TCP(+)$ compared to that of DOCs/${\beta}-TCP(-)$ on rhBMP 10 ng/ml. Our study presented the ${\beta}-TCP$ will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
Keywords
mesenchymal stem cell; rhBMP -2${\beta}-TCP$; differentiated osteoblast-like cell;
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1 Takahashi Y, Yamamoto M, Tabata Y. Enhanced osteoinduction by controlled release of bone morphogenetic protein-2from biodegradable sponge composed of gelatin and beta-tricalcium phosphate. Biomaterials 2005;26:4856-65   DOI   ScienceOn
2 Chu TM, Warden SJ, Turner CH, Stewart RL: Segmental bone regeneration using a load-bearing biodegradable carrier of bone morphogenetic protein-2. Biomaterials 2007;28:459-67   DOI   ScienceOn
3 Mastrogiacomo M, Muraglia A, Komlev V, Peyrin F, Rustichelli F, Crovace A, Cancedda R: Tissue engineering of bone: search for a better scaffold. Orthod Craniofac Res 2005;8:277-84   DOI   ScienceOn
4 Li YL, Xiao ZS: Advances in Runx2 regulation and its isoforms. Med Hypotheses 2007;68:169-75   DOI   ScienceOn
5 Hunter GK, Goldberg HA: Modulation of crystal formation by bone phosphoproteins: role of glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein. Biochem J 1994;302:175-79   DOI
6 Borsalino G, Bagnacani M, Bettati E, Fornaciari F, Rocchi R, Uluhogian S,Ceccherelli G, Cadossi R, Traina GC: Electrical stimulation of human femoral intertrochanteric osteotomies. Clin Orthop Relat Res 1988;237:256-63
7 Chen F, Feng X, Wu W, Ouyang H, Gao Z, Cheng X et al :Segmental bone tissue engineering by seeding osteoblast precursor cells into titanium mesh-coral composite scaffolds. Int J Oral Maxillofac Surg 2007;36:822-27   DOI   ScienceOn
8 Fisher LW, McBride OW, Termine JD, Young MF: Human bone sialoprotein. Deduced protein sequence and chromosomal localization. J Biol Chem 1990;265:2347-51
9 Swallow DM, Povey S, Parkar M: Mapping of the gene coding for the human liver/bone/kidney isoenzyme of alkaline phosphatase the chromosome 1. Ann Hum Genet 1986;50:229-35   DOI   ScienceOn
10 Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem 1997;67:386-98   DOI   ScienceOn
11 Mu¨ller P, Bulnheim U, Diener A, Luthen F, Teller M, Klinkenberg ED, Neumann HG, Nebe B, Liebold A, Steinhoff G, Rychly J: Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells. J Cell Mol Med. 2008;12:281-91   DOI   ScienceOn
12 Kinoshita K, Hibi H, Yamada Y, Ueda M: Promoted new bone formation in maxillary distraction osteogenesis using a tissue-engineered osteogenic material. J Craniofac Surg 2008;19:80-7   DOI
13 Caplan AI: Mesenchymal stem cells. J Orthop Res 1991;9:641-50   DOI
14 Beresford JN: Osteogenic stem cells and the stromal system of bone and marrow. Clin Orthop Rel Res 1989;240:270-80
15 Wiltfang J, Schlegel KA, Schultze-Mosgau S, Nkenke E, Zimmermann R, Kessler P: Sinus floor augmentation with betatricalciumphosphate (beta-TCP): does platelet-rich plasma promote its osseous integration and degradation? Clin Oral Implants Res 2003;14;213-8   DOI   ScienceOn
16 Aghaloo TL, Moy PK, Freymiller EG: Evaluation of platelet-rich plasma in combination with anorganic bovine bone inthe rabbit cranium: a pilot study. Int J Oral Maxillofac Implants 2004;19:59-65
17 Haynesworth SE, Goshima J, Goldberg VM, Caplan AI: Characterization of cells with osteogenic potential from human marrow. Bone 1992;13:81-8   DOI   ScienceOn
18 Hastings GW, Mahmud FA: Electrical effects in bone. J Biomed Eng 1988;10:515-21   DOI
19 Hughes FJ, Collyer J, Stanfield M, Goodman SA: The effects of bone morphogenetic protein-2, -4, and -6 on differentiation of rat osteoblast cells in vitro. Endocrinology 1995;136:2671-7   DOI   ScienceOn
20 Bjerre L, B¨unger CE, Kassem M, Mygind T: Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds. Biomaterials 2008;29:2616-27   DOI   ScienceOn
21 Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR: Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46   DOI   ScienceOn
22 Oklund SA, Prolo DJ, Gutierrez RV, King SE: Quantitative comparisons of healing in cranial fresh autografts, frozen autografts and processed autografts, and allografts in canine skull defects. Clin Orthop Relat Res 1986;205:269-91
23 Goldberg VM, Stevenson S: Nature history of autograft and allograft. Clin Orthop 1987;225: 7-16
24 Ichida F, Nishimura R, Hata K, Matsubara T, Ikeda F, Hisada K, Yatani H, Cao X, Komori T, Yamaguchi A, Yoneda T: Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation. J Biol Chem 2004;279:34015-22   DOI   ScienceOn
25 Banfi A, Bianchi G, Notaro R, Notaro R, Luzzatto L, Cancedda R, Quarto R : Replicative aging and gene expression in long-term cultures of human bone marrow stromal cells. Tissue Eng 2002;8:901-10   DOI   ScienceOn
26 Sato Y, Takita H, Ohata N, Tamura M, Kuboki Y: Pleiotrophin regulates bone morphogenetic protein (BMP)-indued ectopic osteogenesis. J Biochem 2002;131:877-86   DOI   ScienceOn
27 Chaudhary LR, Hofmeister AM, Hruska KA: Differential growth factor control of bone formation through osteoprogenitor differentiation. Bone 2004;34:402-11   DOI   ScienceOn
28 Wakitani S, Goto T, Pineda SJ, Young RG, Mansour JM, Goldberg VM, Caplan AI: Mesenchymal cell-based repair of large full-thickness defects of articular cartilage. J Bone Joint Surg 1994;76:579-92   DOI
29 Jingushi S, Urabe K, Okazaki K, Hirata G, Sakai A, Ikenoue T, Iwamoto Y: Intramuscular bone induction by human recombinant bone morphogenetic protein-2 with beta-tricalcium phosphate as a carrier: in vivo bone banking for muscle-pedicle autograft. J Orthop Sci 2002;7:490-4   DOI
30 Christodoulou I, Buttery LD, Saravanapavan P, Tai G, Hench LL, Polak JM : Dose- and time-dependent effect of bioactive gelglass ionic-dissolution products on human fetal osteoblast-specific gene expression. J Biomed Mater Res B Appl Biomater 2005;74:529-37
31 Fromigue O, Marie PJ, Lomri A: Bone morphogenetic protein-2 and transforming growth factor-beta2 interact to modulate human bone marrow stromal cell proliferation and differentiation. J Cell Biochem 1998;68:411-26   DOI   ScienceOn
32 Langer R, Vacanti JP: Tissue Engineering. Science 1993;260:920-6   DOI
33 Chen Y, Shao JZ, Xiang LX, Dong XJ, Zhang GR: Mesenchymal stem cells: A promising candidate in regenerative medicine. Int J Biochem Cell Biol 2008;40:815-20   DOI   ScienceOn
34 Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilinkage potencial of adult mesenchymal stem cells. Science 1999;284:143-7   DOI   ScienceOn
35 Guillot PV, De Bari C, Dell'accio F, Kurata H, Polak J, Fisk NM: Comparative osteogenic transcription profiling of various fetal and adult mesenchymal stem cell sources. Differentiation 2008;13: in press
36 Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP: Osteogenic differentiation of purified culture expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997:64:295-312   DOI   ScienceOn
37 Becker W: Treatment of small defects adjacent to oral implants with various biomaterials. Periodontol 2000 2003;33:26-35   DOI   ScienceOn
38 Ahn SH, Kim CS, Suk HJ, Lee YJ, Choi SH, Chai JK et al: Effect of recombinant human bone morphogenetic protein-4 with carriers in ratcalvarial defects. J Periodontol 2003;74:787-97   DOI   ScienceOn