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THE mRNA EXPRESSION OF MMP-1, TIMP-1, $TGF-{\beta}_1$ IN GINGIVAL KERATOCYTES FROM GINGIVAL HYPERPLASIA INDUCED BY CYCLOSPORINE A  

Kang, Hag-Soo (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University)
Lee, Jae-Sun (Institute of Biomedical Science, College of Medicine, Hanyang University)
Bing, Jung-Ho (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University)
Park, Chang-Joo (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University)
Im, Jae-Jung (Division of Orthodontics/Department of Dentistry, College of Medicine, Hanyang University)
Hwang, Kyung-Gyun (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University)
Shim, Kwang-Sup (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.34, no.4, 2008 , pp. 405-411 More about this Journal
Abstract
Purpose : Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and $TGF-{\beta}_1$ and the concentration of CsA in cultured human gingival keratinocytes. Materials & Methods : Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and $TGF-{\beta}_1$ were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results : The expressions of MMP-1 and $TGF-{\beta}_1$ were not significantly different according to the concentrations of CsA. The expression of TIMP-1 was significantly increased at the CsA concentration of 500 ng/ml. Conclusion : We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or $TGF-{\beta}_1$ on gingival collagen metabolism in patients treated with CsA.
Keywords
MMP-1; TIMP-1$TGF-{\beta}_1$; cyclosporine A; gingival keratinocyte;
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Times Cited By KSCI : 2  (Citation Analysis)
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