Browse > Article

THE BONE FORMATION AROUND ANODIC OXIDIZED TITANIUM IMPLANTS IN THE TINBIAE OF OVARECTOMIZED RATS  

Park, Sung-Hwan (Soo Boo Boo Dental Clinic)
Jung, Suk-Young (Dept. of Oral and Maxillofacial Surgery, Busan St. Mary's Medical Center)
Lee, Jae-Yeol (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Kim, Gyoo-Cheon (Dept. of Oral Anatomy, College of Dentistry, Pusan National University)
Shin, Sang-Hun (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.34, no.3, 2008 , pp. 306-318 More about this Journal
Abstract
Anodic spark deposition method(ASD) surface treated titanium implant possesses a considerable osteoconductive potential that promoting a high level of implant osseointegration in normal bone. The purpose of this study was to observe the ASD implant's osseointegration in the osteoporosis-induced animal model. Twenty four rats, 10 weeks of age, were ovarectomized and 5 weeks later divided into two groups : ASD implant group and control implant group. Titanium screw implants (diameter; 2.0 mm, length, 3.5 mm; pitch-height, 0.4 mm) were designed for this study. Experimental implants were ASD treated and no treatment on control implants. ASD implants and control implants were placed in to left tibiae of rats. The rats were sacrificed at different time interval(1, 2, 4 and 8 weeks after implantation) for histopathologic observation and immunohisto-chemistrical observation, with collagen type Ⅰ, fibronectin, integrin ${\alpha}_2{\beta}_1$ and integrin ${\alpha}_5{\beta}_1$ antibodies. The results obtained from this study were as follow: 1. Histopathologic findings, overall tissue response and the pattern of bone formation in both groups were similar. In ASD group, more newly formed bone was seen at 1 week and 2weeks than control group. 2. The levels of type Ⅰ collagen and fibronectin expression were the most abundant at 2weeks and decreased gradually in both groups. Fibronectin and type Ⅰ collagen expression in ASD group were stronger than control group but no significance. 3. The levels of integrin ${\alpha}_2{\beta}_1$ and Integrin ${\alpha}_5{\beta}_1$ expression were most abundant at 2 weeks and decreased gradually in both groups. No significant difference was observed in both groups. From this results, anodic oxidized titanium implants were more advantages in early stage of bone formation than control group, but have no significance in tissue responses and late bone formations. It could be stated that although anodic oxidized titanium implant possesses considerable osteoconductive potential but in osteoporotic bone condition dental implant procedure should performed after improving or treating the osteoporotic bone condition.
Keywords
Anodic oxidation(anodization); Osteoporosis; Bone healing; Dental implants;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 통계청: 2005 고령자 통계: 대한민국 통계청. 대전, 2005
2 Riggs BL, Melton LJ: The prevention and treatment of osteoporosis. New Eng J Med 1992;27:620-627
3 Fini M, Giavaresi G, Rimondini L et al: Titanium alloy osseointegration in cancellous and cortical bone of ovariectomized animals; histomorphometric and bone hardness measurements. Int J Oral Maxillofac Implants 2002;17:28-37
4 Johnson RB, Gilbert JA, Cooper RC, et al: Alveolar bone loss one year following ovariectomy in sheep. J Periodontol 1997;68(9):864-871   DOI   ScienceOn
5 Pilliar RM, Deporter DA, Watson PA, Todescan R: The Endopore implant enhanced osseointegration with a sintered porous-surfaced design. Oral Health 1998;88:61-64
6 Caulier H, Vercaigne S, Naert I, et al: The effect of Ca-P plasmasprayed coatings on the initial bone healing of oral implants: An experimental study in the goat. J Biomed Mater Res 1997;34:121-128   DOI
7 Wennerberg A, Albrektsson T, Lausmaa J: Torque and histomorphometric evaluation of c.p. titanium screws blasted with 25- and 75-microns-sized particles of Al2O3. J Biomed Mater Res 1996;30:251-260   DOI
8 Baron R, Tross R, Vignery A: Evidence of sequential remodeling in rat trabecular bone: morphology, dynamic histomorphometry, and changes during skeletal maturation. Anat Rec 1984;208:137-145   DOI   ScienceOn
9 Moursi AM, Damsky CH, Lull JC, et al : Fibronectin regulates calvarial osteoblast differentiation. J Cell Sci 1996;109:1369-1380
10 Ichinohe T, Takayama H, Ezumi Y et al: Collagen-stimulated activation of Syk but not c-Src is severely compromised in human platelets lacking membrane glycoprotein VI. J Biol Chem 1997;272:63-68   DOI
11 민용기: 골흡수의 생화학적 표지자. 대한골대사학회지 1994;1:240-245
12 Liotta LA: Tumor invasion and metastases-role of the extracellular matrix. Rhoads Memorial Award Lecture Cancer Res 1986;46:1-7
13 Pytela R, Pierschbacher MD and Ruoslahti E: Identification and isolation of a 140 kilodalton cell surface glycoprotein with properties of a fibronectin receptor. Cell 1985;40:191-198   DOI   ScienceOn
14 Steinemann SG: Titanium- the material of choice? Periodontology 2000 1998;17:7-20   DOI   ScienceOn
15 Biesbrock AR, Edgerton M: Evaluation of the clinical predictability of hydroxyapatite-coated endosseous dental implants: A review of the literature. Int J Oral Maxillofac Implants 1995;10:712-720
16 Thiery JP, Duband JL and Tucker GC: Cell migration in the vertebrate embryo: role of cell adhesion and tissue environment in pattern formation. Ann Rev Cell Biol 1985;1:91-113   DOI   ScienceOn
17 Kribbs PJ, Chestnut CH, Ott SM, Kilcoyne R: Relationship between mandibular and skeletal bone in osteoporotic population. J Prosthet Dent 1989;62:703-704   DOI   ScienceOn
18 Turner AS, Mallinckrodt CH, Alvis MR, Bryant HU: Dose-response effects of estradiol implants on bone mineral density in ovariectomized ewes. Bone 1995;17(4):421-427
19 Owen TA, Aronow M, Shalhoub V et al: Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 1990;143(3):420-30   DOI
20 보건복지부: 보건복지통계연보: 대한민국 보건복지부. 과천, 2004
21 Clokie CM, Warshawsky H: Development of a rat tibia model for morphological studies of the interface between bone and a titanium implant. Compendium 1995;16:56-66
22 Kalu DN: The ovariectomized rat model of postmenopausal bone loss. Bone and Mineral 1991;15:175-192   DOI   ScienceOn
23 Lynch MP, Stein JL, Stein GS et al: The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. Exp Cell Res 1995;216(1):35-45   DOI   ScienceOn
24 Ivanoff CJ, Widmark G, Johansson C et. al: Histologic evaluation of bone response to oxidized and turned titanium micro-implants in human jawbone. Int J Oral Maxillofac Implants 2003;18:341-348
25 한은영, 류인철, 이용무 등: 골다공증과 악골의 골밀도 및 치주 질환과의 상관관계. 대한치주과학회지 2001;31(3):565-570
26 Meredith N: Assessment of implant stability as a prognostic determinant. Int J Prothodont 1998;11:491-501
27 Glauser R, Portmann M, Ruhstaller P et. al: Stability measurements of immediately loaded machined and oxidized implants in the posterior maxilla(A comparative clincal study using resonace frequency analysis). Applied Osseointegration Research. 2001;2(1):27-29
28 Rocci A, Martignoni M, Burgos PM et al: Histology of retrieved immediately and early loaded oxidized implants: light microscopic observation after 5 to 9 months of loading in the posterior mandible. Clinical Implant Dentistry and Related Research 2003;5(Suppl 1):88-98   DOI   ScienceOn
29 Kribbs PJ, Chesnut CH III, Ott SM, Kilcoyne RF: Relationships between mandibular and skeletal bone in an osteoporotic population. J Prosthet Dent 1989;62:703-7   DOI   ScienceOn
30 Fini M, Cigada A, Rondelli G et al: In vitro and in vivo behavior of Ca and P enriched anodized implants. Biomaterials 1999;20:1587-1594   DOI   ScienceOn
31 Kohn DH: Overview of factors important in implant design. J Oral Implantol 1992;18:204-219
32 통계청: 장래인구추계: 대한민국 통계청. 서울, 2001
33 Friberg B, Billstrom C: Preliminary results of a prospective multicenter clinical study on TiUnite implants. Applied Osseointegration Research 2002;3(1):29-31
34 Keely PJ and Parise LV: The alpha2beta1 integrin is a necessary co-receptor for collagen-induced activation of Syk and the subsequent phosphorylation of phospholipase Cgamma2 in platelets. J Biol Chem 1996;271(43):26668-26676   DOI   ScienceOn
35 von Wowern N, Kollerup G: Symptomatic osteoporosis : a risk factor for residual ridge reduction of the jaws. J Prosthet Dent 1992;67:656-660   DOI   ScienceOn
36 윤상호, 김정구: 폐경 후 골다공증의 최신 치료 : 대한산부회지 2005;48(4):844-857
37 Klemetti E, Vainio P, Lassila V, Alhava E: Trabecular bone mineral density of mandibule and alveolar height in postmenopausal women. Scand J Dent Res 1993;101:166-170
38 Nordahl J, Mengarelli-Widholm S, Hultenby K, et al: Ultrastructural Immunolocalization of Fibronectin in Epiphyseal and Metaphyseal Bone of Young Rats. Calcif Tissue Int 1995;57:442-449   DOI
39 Xiaolong Zhu, Jun Chen, Lutz Scheideler et al: Effect of topography and composition of titanium surface oxides on osteoblast responses. Biomaterials 2004;25:4087-4103   DOI   ScienceOn
40 Hay ED: Cell biology of extracellular matrix. 2nd eds. Plenum press, New York and London, pp111-124, 1991
41 Cook SD, Thomas KA, Dalton JE et al: Hydroxylapatite coating of porous implants improves bone ingrowth and interface attachment strength. J Biomed Mater Res 1992;26:989-1001   DOI
42 Cochran DL, Schenk RK, Lussi A et al: Bone response to unloaded and loaded titanium implants with a sandblasted and acidetched surface: A histometric study in the canine mandible. J Biomed Mater Res 1998;40:1-11   DOI   ScienceOn
43 Svetlana Lim, Seong-Joo Heo, Chong-hyun Han et al: Histologic response of anodized titanium implant. 대한치주과학회지 2005;35(3):525-535   과학기술학회마을   DOI