Browse > Article

THE HISTOMORPHOMETRIC STUDY ON THE HEALING PROCESS OF A MANDIBULAR FRACTURE IN THE STREPTOZOTOCIN-INDUCED DIABETIC RATS  

Kang, Hee-Jea (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Kim, Yong-Deok (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Shin, Sang-Hun (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Kim, Uk-Kyu (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Kim, Jong-Ryoul (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Chung, In-Kyo (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.32, no.3, 2006 , pp. 250-261 More about this Journal
Abstract
Diabetes mellitus, as a major health problem for the elderly, is associated with an extensive list of complications involving nearly every tissue in the body and has been shown to alter the properties of bone and impair fracture healing in both human and animals. The objective of this study was to examine the healing process of a mandibular fracture in the streptozotocin-induced rats histomorphometrically and histologically. A standardized fracture model was chosen and based on blood-glucose value at the time of surgery. A total of 11-weeks old 36 rats were divided into 2 groups; One is a streptozotocin-induced diabetic group and the other is a non-diabetic group. All was fractured experimentally. Three animals from each group were killed 1, 2, 4, 6, 8 and 12 weeks after fracture and specimens were processed undecalcified for quantitative bone histomorphometric and histologic studies. The diabetic group showed a significant decrease of histomorphometry-based parameter including trabecular bone volume, trabecular thickness in comparison to the non-diabetic rat. This was confirmed histologically. In conclusion, this study suggests that in streptozotocin-induced diabetics, the healing process of bone fracture was impaired and delayed about 2-3 weeks comparing to non-diabetics.
Keywords
DM; Streptozotocin; Fracture;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lieberman D, Fried V, Castel H, Weitzmann S, Lowenthal MN, Galinsky D: Factors related to successful rehabilitaion after hip fracture: a case-control study. Disabil Rehabil 1996;18:224-230   DOI   ScienceOn
2 Macey LR, Kana SM, Jingushi S, Terek RM, Borretos J, Bolander ME: Defects of early fracture-healing in experimental diabetes. J Bone Joint Surg 1989;71:722-733   DOI
3 Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Maluche H: Bone histmorphometry: standardisation of nomenclature, symbols, and units. J Bone Miner Res 1987;2:595-610   DOI   ScienceOn
4 McKibbin B: The biology for fracture healing in long bones. J Bone Joint Surg 1978;60:150-162
5 Varmarken JE, Olsen CA, Kristiansen B: Multiple fractures in a young diabetic patient. Injury 1988;19:285-286   DOI   ScienceOn
6 Bain S, Ramamurthy NS, Impeduglia T, Scloman S, Golub LM: Tetracycline prevents calcellous bone loss and maintains near-normal rates of bone formation in streptozotocin diabetic rats. Bone 1997;21:147-153   DOI   ScienceOn
7 Brownlee M, Cerami A, Vlassara H: Advanced glycosylation end products in tissue and the biochemical basis of diabetic complications. N Engl J Med 1988;318:1315-1321   DOI   ScienceOn
8 Berenson GS, Radhakrishnamurthy B, Dalfered ER: Connective tissue macromolecular changes in rats with experimentally induced diabetes. Diabetes 1972;21:733-743   DOI
9 Glajchen N, Epstein S. Ismail F, Thomas S, Fallon M: Bone mineral metabolism in experimental diabetes: Osteocalcin as a measure of bone remodeling. Endocrinology 1988;123:290-295   DOI   ScienceOn
10 Follak N, Kloting I, Wolf E, Merk H: Improving metabolic control reverse the histomorphometric and biomechanical abnormalities of an experimentally induced bone defect in spontaneously diabetic rats. Calcif Tissue Int 2004;74:551-560   DOI
11 Lane JM, Suda M, Timple: Immunofluoroscent localization of structural collagen types in endochondral fracture repair. J Orthop Res 1986;4:318-329   DOI   ScienceOn
12 Albright F, Reifenstein EC: The parathyroid glands and metabolic bone disease: selected studies. William & Willkins Co., 1948, pp 150-152
13 Mishima N, Sahara N, Shirakawa M, Ozawa H: Effect of streptozotocin- induced diabetes mellitus on alveolar bone deposition in the rat. Arch Oral Biol 2002;47:843-849   DOI   ScienceOn
14 Follak N, Kloting I, Wolf E, Merk H: Delayed remodeling in hte early period of fracture healing in spontaneously diabetic BB/OK rats depending on the diabetic metabolic state. Histol Histopathol 2004;19:473-486
15 Schwartz AV, Sellmeyer DE, Ensrud KE, Cauley JA, Tabor HK: Older women with diabetes have an increased risk of fracture: a prospective study. J Clin Endocrinol Metab 2001;86:32-8   DOI
16 Devlin H, Garland H, Sloan P: Healing of tooth extraction sockets in experimental diabetes mellitus. J Oral Maxillofac Surg 1996;54:1087- 1091   DOI   ScienceOn
17 Loder RT: The influence of diabetes mellitus on the healing of closed fractures. Clin Orthop 1988;232:210-216
18 Herbsman H, Power JC, Hirschman A, Shaftan GW: Retardation of fracture healing in experimental diabetes. J Surg Res 1968;8:424-431   DOI   ScienceOn
19 Levin ME, Boisseau VC, Avioli LV: Effects of diabetes mellitus on bone mass in juvenile and adult-onset diabetes. N Engl J Med 1976;294:241-245   DOI   ScienceOn
20 Shyng YC, Devlin H,Sloa P: The effect of streptozotocin-induced experimental diabetes mellitus on calvarial defect healing and bone turnover in the rat. Int J Oral Maxillofac Surg 2001;30:70-74   DOI   ScienceOn
21 Cozen L: Does diabetes delay fracture healing? Clin Orthop 1972;82:134-140
22 Seifter E, Rettura G, Padawer J, Stratford F, Kambosos D, Levenson SM: Impaired wound healing in streptozotocin diabetes: prevention by supplemental vitamin A. Ann Surg 1981;194:42-50   DOI   ScienceOn
23 Yamada Y, Fushimi H, Inou T: Effects eicosapentaenoic acid and docosaheaenoic acid and diabetic osteopenia. Diabetes Res Clin Pract 1995;30:37-42   DOI   ScienceOn
24 Ramamurthy NS, Golub LM: Diabetes increases collagenase activity in extracts of rat gingiva and skin. J Periodent Res 1983;18:23-30   DOI
25 Schwartz AV: Diabetes Mellitus: Does it affect bone? Calcif Tissue Int 2003;73:515-519   DOI
26 Follak N, Kloting I, Wolf E, Harry M: Histomorphometric evaluation of the influence of the diabetic metabolic state on bone defect healing depending on the defect size in diabetic rat. Bone 2004;35:144-152   DOI   ScienceOn
27 Hedlund LJ, Maki DD, Griffiths HJ: Calcaneal fractures in diabetic patients. J Diab Comp 1997;12:81-87   DOI   ScienceOn
28 Thomas T, Burguera B, Melton LJ, Atkinson EJ, O'fallon WM: Role of serum leptin, insulin, and estrogen levels as potential mediators of the relationship between fat mass and bone mineral density in men versus women. Bone 2001;29:114-120   DOI   ScienceOn
29 Beam HJ, Parsons JR, Lin SS: The effects of blood glucose control upon fracture healing in the BB Wistar rat with diabetes mellitus. J Orthop Res 2002;20:1210-1216   DOI   ScienceOn
30 Hollinger J, Wong KE: The integrated processes of hard tissue regeneration with special emphasis on fracture healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1996;82:594-606   DOI   ScienceOn
31 Simmons DJ: Fracture healing perspectives. Clin Orthop 1985;200:100-113
32 Funk JR, Hale JE, Carmines D, Gooch HL: Biomechanical evaluation of early fracture healing in normal and diabetic rats. J Ortho Res 2000;18:126-132   DOI   ScienceOn
33 Mustard JF, Packham M: Platelets and diabetes mellitus. N Engl J Med 1984;311:665-667   DOI   ScienceOn
34 Verhaeghe J, Visser WJ, Einhorn TA, Bouillon R: Osteoporosis and diabetes: Lessons from the diabetic BB rat. Horm Res 1990;34:245-248   DOI
35 McNair P, Madsbad S, Christiansen C, Christiansen MS: Bone loss in diabetes. Diabetologia 1979;17:283-286   DOI
36 Cyran F, Sheldon LS, Gregory GP, Nicholas A, Wayne B, Alfred B: Complications after surgical treatment of diabetic vs non-DM patients with isolated ankle fracture: A cohort study. Diabete Res Clin Prac 2000;50:280
37 Kankauer JC, McKenna MJ, Buderer NF, Rao DS, Whitehouse FW, Parfitt AM: Bone loss and bone turnover in diabetes. Diabetes 1995;44:775-782   DOI   ScienceOn