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THE EFFECTS OF INSULIN-LIKE GROWTH FACTOR I (IGF-I) ON EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) MRNA IN MG-63 OSTEOBLASTLIKE CELLS  

Suh, Je-Duck (Department of Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital)
Myung, Hoon (Department of Oral and Maxillofacial Surgery, Seoul National University, College of Dentistry)
Kang, Nara (Department of Oral and Maxillofacial Surgery, Ewha Womans University, College of Medicine)
Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery, Seoul National University, College of Dentistry)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.31, no.5, 2005 , pp. 363-369 More about this Journal
Abstract
Purpose: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. Materials and methods: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea $(76.1\;{\mu}g/ml)$, actinomycin D $(2.5\;{\mu}g/ml)$, cycloheximide $(10\;{\mu}g/ml)$ were added 1 hour after treatment of 10 nM IGF-I. Results: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time-and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. Conclusion: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.
Keywords
Osteogenesis; Angiogenesis; Vascular endothelial growth factor; Insulin-like growth factor I; MG-63 cell line;
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