Browse > Article

THE CHANGES OF TELOMERASE ACTIVITY AND PROLIFERATING CELL NUCLEAR ANTIGEN(PCNA) EXPRESSION IN THE DEVELOPMENTAL STAGES OF RAT'S UPPER DIGESTIVE SYSTEM  

Sung, Iel-Yong (Dept. of Oral & Maxillofacial Surgery, College of Medicine, Ulsan University)
Kim, Jong-Ryoul (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.30, no.2, 2004 , pp. 85-99 More about this Journal
Abstract
Purpose: This study observed the changes in the telomerase activity, it's developmental regulation, PCNA expression, and their correlation in rat's upper digestive organs during growth and aging. Materials and Methods: Upper digestive organs(buccal mucosa, gingiva, palate, submandibular and parotid glands, and tongue) were aseptically removed from Sprague-Dawley rats of fetal(gestational 20 days), growing(1, 2, 3, 5, and 7 weeks after birth) and adult(12 week old). Samples for telomerase activity were frozen on liquid nitrogen immediately after sacrifice, and stored until the use at $-75^{\circ}C$ in order to measure it. Telomerase activity was measured by a PCR-based telomeric repeat amplication protoco(TRAP) assay and quantitated with Photometric Telo TAGGG Telomerase PCR ELISA plus(Roche Diagnostics GmbH. Mannheim. Germany). PCNA expression were measured immunohistochemistry with anti PCNA Ab-1, Clone PC10(NeoMark. California. USA). Results: 1. Telomerase activities in buccal mucosa, palate and gingiva were the highest in fetus and decreased gradually or rapidly after birth and then diminished, but In salivary gland and tongue were the highest in fetus and also high at 1 week and then decreased rapidly. 2. PCNA expression in buccal mucosa, gingiva, Tongue and salivary gland was the highest in fetus and decreased gradually and then diminished. but only in palate decreased rapidly after birth and then diminished. Conclusion: The highest telomerase activity of embryonic stage decreased rapidly after birth in rat's upper digestive organs. There may be a developmental regulation of telomerase activity, but not a tissue-specific. This telomerase activity seems correlated closely with PCNA expression in rat's upper digestive system.
Keywords
Telomerase; PCNA; Upper digestive system;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim NW, Wu F: Advances in quantification and characterization of telomerase activity by the telomeric repeat amplication protocol(TRAP). Nucleic Acids Res 25:2595-7, 1997   DOI   ScienceOn
2 Toschi L, Bravo R: Changes in cyclin/PCNA distribution during DNA repair synthesis. J Cell Biol 107:1623-8, 1988
3 Blasco MA, Funk W, Villeponteau B, Greider CW: Functional characterization and developmental regulation of mouse telomerase RNA. Sci 269: 1267-70, 1995
4 Zakian VA: Structure and function of telomeres. Annu Rev Genet 23:579-604, 1989
5 Takubo K, Nakamura N, Mafune K, Tanaka Y, Miyashita et al: Telomerase activity in esophageal carcinoma. J Surg Onco 66:88-92, 1997
6 Landberg G, Nielsen NH, Nilsson P, Emdin SO, Cajandr J, et al: Telomerase activity is associated with cell cycle deregulation in human breast cancer. Cancer Res 57:549-54, 1997
7 Yasumoto S, Kunimura C, Kikuchi K, Tahara H, Ohji H, et al: Telomerase activity in normal human epithelial cells. Oncogene 13:433-9, 1996
8 Liao J, Mitsuyasu T, Yamana K, Ohishi M: Telomerase activity in oral and maxillofacial tumors. Oral Onco 36:347-52, 2000
9 Blackburn EH: Structure and function of telomeres. Nature 350:569-573, 1991
10 Kannan S, Tahara H, Yokozaki H, Mathew B, Nalinakumari KR, et al: Telomerase activity in premalignant and lesions of human oral mucosa. Cancer Epidemiol Biomarkers Prev 6:413-20, 1997
11 Cohen MB, Waldman FM, Carrol PR, Kerschmann R, Chew K, et al: Comparison of five histopathologic methods to assess cellular proliferation in transitional cell carcinoma of the urinary bladder. Hum Pathol 24;411-86, 1993
12 Morin GB: The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59:521-9, 1989
13 Mao L, El-Naggar AK, Fan YH, Lee JS, Lippman SM, et al: Telomerase activity in head and neck squamous cell carcinoma and adjacent tissues. Cancer Res 56:5600-4, 1996
14 Greider CW, Blackburn EH: The telomere terminal transferase of tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell 51:887-98, 1987
15 Wright WE, Piatyszek MA, Ranley WE, Byrd W, Shay JW: Telomerase activity in human germline and embryonic tissues and cells. Dev Genet 18:173-9, 1996
16 Greider CW: Telomere, telomerase and senescence. Bioessays 12:363-9, 1990
17 오금수, 이상돈: 백서 신장의 발달 시기별 Telomerase 활성 및 Proliferating Cell Nuclear Antigen(PCNA) 발현에 관한 연구. 대한비뇨기과학회지 41:1041-50, 2000
18 Mutiranguna A, Supiyaphun P, Trirekapan S, Sriuranpong V, Sakuntabhai A, et al: Telomerase activity in oral leukoplakia and head and neck squamous cell carcinoma. Cancer Res 56:3530-3, 1996
19 Cerni C: Telomeres, telomerases, and myc. An update. Mutation Res 462:31-47, 2000
20 Sumida T, Sogawa K. Hamakawa H, Sugita A, Tanioka H, et al: Detection of telomerase activity in oral lesions, J Oral pathol Med 27:111-5, 1998
21 Brestilny L, Brown CB, Miura Y, Robertson LD, Riabowol KT: Selective inhibition of telomerase activity during terminal differentiation of immortal cell lines. Cancer Res 56: 3796-802, 1996
22 Li M, Adel K, El-Naggar, Fan YH, Lee JS, et al: Tolmerase activity in head and neck squamous cell carcinoma and adjacent tissues, Cancer Res 56:5600-4, 1996
23 Yamaguchi Y, Nozawa K, Savcysky E, Hayakawa N, Nimura Y, et al: Change in telomerase of rat organs during growth and aging. Exp Cell Res 242:120-7, 1998   DOI   ScienceOn
24 Landberg G, Tan EM, Roos G: Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen. Exp Cell Res 187:111-8, 1990
25 Harley CB, Villeponteau B: Telomeres and telomerase in aging and cancer. Curr Opin Genet Dev 5: 249-55, 1995
26 Meyer JS, Coplin MD: Thymidine labeling index, flow cytometric sphase measurement, and DNA index in human tumors: Comparison and correlations. Am J Clin Pathol 89:586-95, 1988
27 Prowse KR, Greider CW: Developmental and tissue -specific regulation of mouse telomerase and telomere length. Proc Natl Acad Sci USA 92:4818-22, 1995
28 Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, et al: Specific association of human telomerase activity with immortal cells and cancer. Sci 266:2011-5, 1994