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BONE FORMATION BY HUMAN ALVEOLAR BONE CELLS  

Choi, Byung-Ho (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University)
Park, Jin-Hyoung (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University)
Huh, Jin-Young (Dept. of Dentistry, Asan Kangnung Hospital)
Oh, Jin-Rok (Dept. of Orthopedics, Wonju College of Medicine, Yonsei University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.28, no.1, 2002 , pp. 42-45 More about this Journal
Abstract
Cultures of primary human alveolar bone-derived cells were established from alveolar bone chips obtained from normal individuals undergoing tooth extraction. These cells were expanded in vitro until passage 3 and used for the in vivo assays. Cells were loaded into transplantation vehicles, and transplanted subcutaneously into immunodeficient mice to study the capacities of human alveolar bone-derived cells to form bone in vivo. Transplants were harvested 12 weeks after transplantation and evaluated histologically. Of 10 human alveolar bone-derived cell transplants, two formed a bone-like tissue that featured osteocytes and mineral. Eight of the ten formed no osseous tissue. These results show that cells from normal human alveolar bone are capable of forming bone-like tissue when transplanted into immunodeficient mice.
Keywords
Tissue engineering; Cell culture; Alveolar bone;
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