Browse > Article

Antibacterial Effect on Oral Normal flora of Phytoncide from Chamaecyparis Obtusa  

Auh, Q-Schick (Department of Oral Medicine, School of Dentistry, Kyung Hee University)
Hong, Jung-Pyo (Department of Oral Medicine, School of Dentistry, Kyung Hee University)
Chun, Yang-Hyun (Department of Oral Medicine, School of Dentistry, Kyung Hee University)
Publication Information
Journal of Oral Medicine and Pain / v.34, no.4, 2009 , pp. 353-362 More about this Journal
Abstract
The present study was performed to observe the effect of phytoncide on oral normal microflora and the inhibitory effect of the surviving resident oral bacteria on F. nucleatum. In this study, saliva from each of 20 healthy subjects was treated with 1% phytoncide from Japanese Hinoki (Chamaecyparis obtusa Sieb. et Zucc.). The surviving salivary bacterium were isolated on blood agar plates and identified by 16S rDNA sequencing. In order to select inhibitory isolates against F. nucleatum, the isolates from the phytoncide-treated saliva were cultured with F. nucleatum. The results are as follows: 1. Among the 200 surviving resident oral bacterium, 70(35.0%) bacterium inhibit the growth of F. nucleatum on blood agar plates. 2. Among the 70 bacterium which inhibit F. nucleatum, Streptococcus salivarius was 41.3%(45/109), Streptococcus sanguinis was 28%.(7/25), Streptococcus mitis was 20%(3/15), Streptococcus parasanguinis was 33.3%(3/9), Streptococcus Alactolyticus was 100%(8/8), Streptococcus vestibularis was 28.6%(2/7) and Streptococcus sp. was 50%(2/4). Taken together, among the surviving resident oral bacterium, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus mitis were mainly observed to inhibit F. nucleatum. and they may exert an additional inhibitory activity against the periodontopathic bacterium. Therefore, phytoncide can be used to prevent and cease the progress of periodontal disease, halitosis. Thus it is expected to promote oral health.
Keywords
Oral normal flora; Phytoncide; Antibacterial effect; F. nucleatum;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 강수경, 신미경, 어규식, 전양현, 홍정표. 구강병원균에 대한 편백 피톤치드의 항균작용. 대한구강내과학회지 2007;32:45-55
2 Welsh C. Complementary therapies in hospice care:touch with oils-a pertinent part of holistic care. Am J hospice Palliat Care 1997;14:42-44   DOI   ScienceOn
3 Lis-Balchin M. Essential oils and aromatherapy: their modern role in healing. J R Soc Health 1977;117: 324-329   DOI
4 Rosan B, Lamont RJ. Dental plaque formation. Microbes Infect 2000;2:1599-1607   DOI   PUBMED   ScienceOn
5 Muller CH. Allelopathy as a factor in ecological process. Vegetatio 1969;18:348-357   DOI
6 강하영, 최인규, 정의배, 윤영원, 나기정, 오종환. 침엽수종으로부터 분리된 정유의 스트레스 완화효과. 한국실험동물학회지 1998;14(1)93-96
7 이현옥, 백승화, 한동민. 편백정유의 항균효과. Kor J Appl Microbiol Biotechnol 2001;29:253-257
8 Cox SD, Mann CM, Markham JL et al. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol 2000;88:170-175   DOI   PUBMED   ScienceOn
9 Nguefack J, Budde BB, Jakobsen M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett Appl Microbiol 2004;39: 395-400   DOI   PUBMED   ScienceOn
10 Schnaubelt K. Functional group therapy. The International Journal of Aromatherapy 2000;10:62-63   DOI   ScienceOn
11 이종흔, 김중수. 구강생리학. 3판, 서울, 1989, 군자출판사, pp204-206
12 Morgan TD, Beezer AE, Mitchell JC, Bunch AW. A microcalorimertric comparison of the anti-Streptococcus mutans efficacy of plant extracts and antimicrobial agents in oral hygiene formations. J Appl Microbiol 2001;90: 53-58   DOI   ScienceOn
13 Papapanou PN. Population studies of microbial ecology in periodontal health and disease. Ann Periodontol 2002;7:54-61   DOI   ScienceOn
14 Tessier JF, Kulkarni GV. Bad breadth: etiology and treatment. Periodontic oral health Periodontic oral health 1991;10:19-24
15 이종한. 피부감염모낭충에 대한 피톤시드의 역할. 중앙대학교 학위논문 2005:19-21
16 Carson CF, Mee BJ, Riley TV. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assay and electron microscopy. Antimicrob Agents Chemother 2002;46: 1914-1920   DOI
17 안정엽, 이성숙, 강하영. 편백(Chanaecyparis obtusa) 정유의 항균, 항염, 항산화 효과. J Soc Cosmet Scientists Korea 2004;30(4):503-507
18 Beck JD, Koch GG, Zambon JJ, Genco RJ, Tudor GE. Evaluation of oral bacteria as risk indicators for periodontitis in older adults. J Periodontol 1992;63(2):93-99   DOI   PUBMED
19 Walker CB, Ratliff D, Muller D, Mandell R, Socransk SS. Medium for selective isolation of Fusobacterium nucleatum from human periodontal pockets. J Clin Microbiol 1979;10(6):844-849
20 Scully C, El-Maaythah M, Poter SR, Greenman J. Breadth odor: etiopathogenesis, assessment and management. Eur J Oral Sci 1995;105:287-293   DOI   ScienceOn
21 이진용. 타액단백질과 치태형성. 타액과 타액선 토론회 1995:27-35
22 박재봉, 어규식, 전양현, 홍정표. 피톤치드 처리 후의 잔존 구강 세균이 Pr. intermedia에 미치는 영향. 대한안면통증구강내과학회지 2009;34(2):153-168
23 강하영, 이성숙, 최인규. 침엽수 수엽 정유의 항균성에 관한 연구. 한국임산에너지학회지 1993;13(2):71-77
24 Whittaker RH, Feeny PP. Alleochemics, chemical interactions between species. Science 1971;171:757-770   DOI   PUBMED
25 미야자키 요시후미. 산림욕과 릴렉션. 후레 그란스자날사 1994, 156-159
26 Vincent JW, Cornett WC, Falkler WA Jr, Montoya RG. Biologic activity of type I and type II Fusobacterium nucleatum isolates from clinically characterized sites. J Periodontol 1985;56(6):334–339   DOI
27 Tonzetich J. Production and origin of oral malodor: a review of mechanism and methods of analysis. J Periodontol 1977;28:13-20
28 Kleinberg I, Westbay G. Salivary methanolic factors and involved in oral malodor formation. J Periodontal 1992;63:768-765   DOI
29 Cowan MM. Plant products as antimcrobial agents. Clin Microbiol Rev 1999;12:564-582   PUBMED
30 강하영, 오종환. 침엽수 침엽 정유의 방향성 이용적성. 임업연보 1994;49:177-179
31 강하영, 정의배, 나기정, 윤신근. 침엽수 정유의 생물학적 효능. 한국실험동물학회지 1999;15(1)79-81
32 이승우 외. 구강진단학. 4판, 서울, 1990, 고문사, pp17
33 Moore WE, Moore LH, Ranney RR, Smibert RM, Burmeister JA, Schenkein HA. The microflora of periodontal sites showing active destructive progression. J Clin Periodontol 1991;18(10):729–739   DOI