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Antibacterial Activity of $NANOVER^{TM}$ Against Oral Malodor Generating Microorganisms 1. The Effect of Nanosilver on Growth of Oral Malodor Generating Microorganisms  

Jung, Young-Hee (Food Science and Nutrition, Daegu Catholic University)
Mo, Hye-Won (Food Science and Nutrition, Daegu Catholic University)
Jeong, Ji-Suk (Food Science and Nutrition, Daegu Catholic University)
Choi, Kyung-Ho (Food Science and Nutrition, Daegu Catholic University)
Choi, Jae-Kap (Department of Oral Medicine, School of Dentistry, Kyungpook National University)
Hur, Yun-Kyung (Department of Oral Medicine, School of Dentistry, Kyungpook National University)
Lee, Sang-Heun (Department of Otolaryngology, School of Medicine, Kyungpook National University)
Publication Information
Journal of Oral Medicine and Pain / v.34, no.1, 2009 , pp. 39-48 More about this Journal
Abstract
Recently there is much interest in the antibacterial activity of nano-sized silver particle (nanosilver) since silver is known to be safe and effective as disinfectant for a long time. Oral malodor is considered to originate in the oral cavity primarily as a result of production of malodorous compounds by oral bacteria. Major compounds responsible for oral malodor are volatile sulfur compounds, which is thought to be generated by the G(-) anaerobic bacteria found normally in the oral cavity, especially on the dorsum of the tongue. The purposes of this study were to investigate the effect of nanosilver on growth of oral malodor generating microorganisms, including Fusobacterium nucleatum, Prevotella melaninogenica, Klebsiella pneumonia, and to determine the optimal culture condition of them. The results were as follows: 1. The optimal culture condition for P. melaninogenica was vacuum culture using desiccator after evacuation of air by vacuum pump in chopped beef meat media. 2. The growth of K. pneumonia was temporarily inhibited by nanosilver (5 ppm and 10 ppm). 3. The morphological alteration and cell damage caused by nanosilver were observed in K. pneumonia.
Keywords
Nasosilver; Oral malodor; G(-) anaerobic bacteria; Antibacterial effect;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Miyazaki H, Sakao S, Katoh Y, Takehara T. Correlation between volatile sulphur compounds and certain oral health measurements in the general population. J Periodontol 1995;66:679-684   DOI   PUBMED   ScienceOn
2 Tonzetich J. Production and origin or oral malodor: a review of mechanisms and methods of analysis. J Periodontol 1977;48:13-20   DOI   PUBMED
3 Yaegaki K, Sanada K. Biochemical and clinical factors influencing oral malodor in periodontal patients. J Periodontol 63:783-789, 1992   DOI   PUBMED
4 Nachnami S. The effects of oral rinses on halitosis. J Cal Dent Assoc 1997;25:145-150   PUBMED
5 Winkel EG, Roldan S, Van Winkelhoff AJ, Herrera D, Sanz M. Clinical effects of a new mouthrinse containing chlorhexidine, cetylpyridinium chloride and zinc-lactate on oral halitosis. J Clin Periodontol 2003;30:300-306   DOI   ScienceOn
6 조준형, 이용원, 김형진, 이종만. 기상분사법을 이용한 은나노 항균 기능지 개발에 관한 연구. 한국펄프.종이 공학회 춘계 학술발표논문집 2005;197-205
7 Berg M, Fosdick LS. Studies in periodontal disease. II. Putrefactive organisms in the mouth. J Dent Res 1946;25:73-81   DOI
8 McNamara TF, Alexander JF, Lee M. The role of microorganisms in the production of oral malodor. Oral Surg 1972;34:41-48   DOI   ScienceOn
9 Bosy A, Kulkarni GV, Rosenberg M, McCulloch CAG. Relationship of oral malodor to periodontitis: evidence of independence in discrete subpopulations. J Periodontol 1994;65:37-46   DOI   PUBMED   ScienceOn
10 De Boever EH, De Uzeda M, Loesche WJ. Relationship between volatile sulfur compounds. BANA-hydrolyzing bacteria and gingival health in patients with and without complaints of oral malodor. J Clin Dent 1994;4:114-119   PUBMED
11 Nakano Y, Yoshimura M, Koga T. Correlation between oral malodor and periodontal bacteria. Microbes Infet 2002;4:679-683   DOI   ScienceOn
12 Tanaka M, Yamamoto Y, Kuboniwa M et al. Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor. Microbes and Infect 2004;6:1078-1083   DOI   ScienceOn
13 Donaldson AC, McKenzie D, Riggio MP et al. Microbiological culture analysis of the tongue anaerobic microflora in subjects with and without halitosis. Oral Dis 2005;11(Suppl 1):61-63   DOI   ScienceOn
14 안형준, 유지원, 김기서, 김종열, 최종훈. 키토올리고당 과 자일리톨 및 아연이 포함된 구강청정제(마이센스액) 의 구취감소, 치태형성 및 재부착 억제 치은염 완화 효과에 대한 연구. 대한구강내과학회지 2003;28(2):185-195
15 Persson S, Claesson R, Carlsson J. The capacity of subgingival species to produce volatile sulfur compounds in human serum. Oral Microbiol Immunol 1989;4:169-172   DOI   ScienceOn
16 Morita M, Wang HL. Relationship between sulcular sulfide level and oral malodor in subjects with periodontal disease. J Periodontol 2001;72:79-84   DOI   ScienceOn
17 Tonzetich, J. Production and origin of oral malodor - A review of mechanisms and methods of analysis. J Periodontol 1977;48:13-20   DOI   PUBMED
18 GoldbergS, Kozlovsky A, Gordon D, Gelernter I, Sintov A, Rosenberg M. Cadaverine as a putative component of oral malodor. J Dent res 1994;73(6):1168-1172   DOI   PUBMED
19 Chen X, Schluesener. Nanosilver. A nanoproduct in medical application. Toxicology Letters 2008;176:1-12   DOI   ScienceOn
20 Cheng D, Yang J, Zhao Y. Antibacterial materials of silver nanoparticles application in medical appliances and appliances for daily use. Chin Med Equip J 2004;4:26-32
21 Nester EW, Anderson DG, Roberts CE, Jr., Nester MT. Microbiology-a human perspective. 4th ed., 2006, McGraw-Hill Co, pp. 88-89
22 Haraszthy VI, Zambon JJ, Sreenivasan PK et al. Identification of oral bacterial species associated with halitosis. J Am Dent Assoc 2007;138:1113-1120   DOI
23 이재명, 임성빈, 정진형, 홍기석. 치주질환 치료 후 구취감소에 대한 연구. 대한치주과학회지 2004;34(2):449-459
24 김도윤, 신금백, 장기완. Chitosan이 구취 관련 구강 세균의 증식에 미치는 억제효과. 대한구강내과학회지 2002;27(1):1-14
25 신승철, 이건수. 한국인의 구취실태에 대한 역학조사연구. 대한구강보건학회지 1999;23(4):343-359
26 Persson S, Edlund MB, Claesson R, Carlsson J. The formation of hydrogen sulfide and methyl mercaptan by oral bacteria. Oral Microbiol Immunol 1990;5(4):195-201   DOI   ScienceOn
27 Washio J, Sato T, Koseki T, Takahashi N. Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodor. J Med Microbiol 2005;54:889-895   DOI   ScienceOn
28 Kleinberg I, Westbay G. Salivary and metabolic factors involved in oral malodor formation. J Periodontol 1992;63:768-775   DOI   PUBMED
29 Klinberg I, Westbay G. Oral malodor. Crit Rev Oral Biol Med 1990;1:247-259   DOI
30 Figueiredo LC, Rosetti EP, Marcantonio Jr. E, Marcantonio RAC, Salvador SL. The relationship of oral malodor in patients with or without periodontal disease. J Periodontol 2002;73:1338-1342   DOI   ScienceOn
31 강은혜, 임회순, 최재흥, 김무영. 전문가 잇솔질과 자가잇솔질의 구취감소효과 비교. Oral Biology Research 2003;27(2):77-84
32 Miyazaki H, Sakao S, Katoh Y, Takehara T. Correlation between volatile sulphur compounds and certain oral health measurements in the general population. J Periodontol 1995;66:679-684   DOI   PUBMED   ScienceOn
33 Yaegaki K, Sanada K. Volatile sulfur compounds in mouth air from clinically healthy subjects and patients with periodontal disease. J Periodont Res 1992;27:233-238   DOI   PUBMED
34 김지연, 김성은, 김재은, 이종찬, 윤제용. 은 이온과의 비교를 통한 나노 은 입자의 항균 특성 연구. 대한환경공학회지 2005;27(7):771-776
35 De Boever EH, Loesche WJ. Assessing the contribution of anaerobic microflora of the tongue to oral malodor. J Am Dent Assoc 1995;126:1384-1393   DOI
36 조경환, 박수길. 초음파법으로 제조된 은 나노 입자의 항균특성 연구. J Korean Ind Eng Chem 2004;15:952-955