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Effect of Glucose at High Concentrations on the Integrin and Cathepsin Expression of the Cultured Human Periodontal Ligament Cells  

Kim, Bang-Soo (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
Shin, Je-Won (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
Hong, Jung-Pyo (Department of Oral Medicine School of Dentistry, Kyung Hee University)
Jue, Seong-Suk (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
Publication Information
Journal of Oral Medicine and Pain / v.33, no.1, 2008 , pp. 35-40 More about this Journal
Abstract
This experiment was designed to clarify the effect of high concentrations of extracellular glucose on the periodontal ligament cells. The cells were incubated for 24 and 48 hours with ${\alpha}-MEM$ including 1,000 mg/L(control group) and 4,500 mg/L(experimental group) of glucose. Then, the expressions of ${\alpha}5$ integrin, cathepsin-B and -L were examined using RT-PCR method. The results were as follows: 1. ${\alpha}5$ integrin expression was increased after incubation in high glucose media. 2. Cathepsin-B expression was increased after the 24-H incubation with high glucose media, but was decreased after the 48-H incubation. 3. The expression of cathepsin-L was decreased by the high glucose media. Theses results suggest that extracellular glucose at high concentration may be attributed to delayed wound healing in diabetes patients through increase in ${\alpha}5$ integrin and decrease in cathepsins, which lead to accumulation of extracellular matrices and adhesion molecules.
Keywords
Glucose; Periodontal ligament cells; Integrin; Cathepsin;
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