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Synthetic Chenodeoxycholic Acid Derivative HS-1200-Induced Apoptosis of Human Oral Squamous Carcinoma Cells  

Kim, In-Ryoung (Department of Biology, College of Natural Science, Pusan National University)
Sohn, Hyeon-Jin (Department of Oral Anatomy, College of Dentistry, Pusan National University)
Kim, Gyoo-Cheon (Department of Oral Anatomy, College of Dentistry, Pusan National University)
Kwak, Hyun-Ho (Department of Oral Anatomy, College of Dentistry, Pusan National University)
Park, Bong-Soo (Department of Oral Anatomy, College of Dentistry, Pusan National University)
Choi, Won-Chul (Department of Biology, College of Natural Science, Pusan National University)
Ko, Myung-Yun (Department of Oral Medicine, College of Dentistry, Pusan National University)
Ahn, Yong-Woo (Department of Oral Medicine, College of Dentistry, Pusan National University)
Publication Information
Journal of Oral Medicine and Pain / v.32, no.3, 2007 , pp. 251-261 More about this Journal
Abstract
Bile acids and synthetic its derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. Previous studies have been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis inducing activity on various cancer cells in vitro. It wasn't discovered those materials have apoptosis induced effects on YD9 human oral squamous carcinoma cells. The present study was done to examine the synthetic bile acid derivatives(HS-1199, HS-1200) induced apoptosis on YD9 cells and such these apoptosis events. We administered them in culture to YD9 cells. Tested YD9 cells showed several lines of apoptotic manifestation such as activation of caspase-3, degradation of DFF, production of poly (ADP-ribose) polymerase(PARP) cleavage(HS-1200 only), DNA degradation(HS-1200 only), nuclear condensation, inhibition of proteasome activity, reduction of mitochondrial membrane potential(HS-1200 only) and the release of cytochrome c and AIF to cytosol. Between two synthetic CDCA derivatives, HS-1200 showed stronger apoptosis-inducing effect than HS-1199. Therefore HS-1200 was demonstrated to have the most efficient antitumor effect. Taken collectively, we demonstrated that a synthetic CDCA derivative HS-1200 induced caspases-dependent apoptosis via mitochondrial pathway in human oral sqauamous carcinoma cells in vitro. Our data therefore provide the possibility that HS-1200 could be considered as a novel therapeutic strategy for human orall squamous carcinoma from its poweful apoptosis-inducing activity.
Keywords
Apoptosis; Synthetic CDCA derivatives; HS-1200; Human oral squamous cell carcinoma;
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1 Zimmermann KC, Bonzon C, Green DR. The machinery of programmed cell death. Pharmacol Ther 2001;92:57-70   DOI   ScienceOn
2 Reed JC. Apoptosis-regulating proteins as targets for drug discovery. Trend Mol Med 2001;5:459-471
3 Jones B, Roberts PJ, Faubion WA, Kominami E, Gores GJ. Cystatin A expression reduces bile salt-induced apoptosis in a rat hepatoma cell line. Am J Physiol 1998;275:723-730
4 Patel T, Bronk SF, Gores GJ. Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes. J Clin Invest 1994;94:2183-2192   DOI   ScienceOn
5 Powell AA, LaRue JM, Batta AK, Martinez JD. Bile acid hydrophobicity is correlated with induction of apoptosis and/or growth arrest in HCT116 cells. Biochem J 2001;356:481-486   DOI   ScienceOn
6 Wyllie AH, Kerr JFR, Currie AR. Cell death: the significance of apoptosis. Int Rev Cytol 1980;68:251-305   DOI
7 Thornberry NA, Rosen A, Nicholson DW. Control of apoptosis by proteases, in Apoptosis (Kaufmann SH eds). Adv Pharmacol 1997;44:155-177
8 Yuan J. Evolutionary conservation of a genetic pathway of programmed cell death. J Cell Biochem 1996;60:4-11   DOI
9 Jeong JH, Park JS, Moon B et al. Orphan nuclear receptor Nur77 translocates to mitochondria in the early phase of apoptosis induced by synthetic chenodeoxycholic acid derivatives in human stomach cancer cell line SNU-1. Ann N Y Acad Sci 2003;1010:171-177   DOI
10 Drexler HC, Risau W, Konerding MA. Inhibition of proteasome function induces programmed cell death in proliferating endothelial cells. FASEB J 2000;14: 65-77   DOI
11 Adams J, Palombella VJ, Sausville EA et al. Proteasome inhibitors: a novel class of potent and effective antitumor agents. Cancer Res 1999;59:2615-2622
12 Park BS, Baek SJ, Song KH et al. Genistein-induced apoptosis of p815 mastocytoma cells is mediated by Bax and augmented by a proteasome inhibitor, lactacystin. Nutr Cancer 2002;42:248-255   DOI   ScienceOn
13 Kroemer G, Zamzami N, Susin SA. Mitochondrial control of apoptosis. Immunol Today 1997;18:44-51   DOI   ScienceOn
14 Williams GT. Programmed cell death: apoptosis and oncogenesis. Cell 1991;65:1097-1098   DOI   ScienceOn
15 Sadoul R, Fernandez PA, Quiquerez AL et al. Involvement of the proteasome in the programmed cell death of NGF-deprived sympathetic neurons. EMBO J 1996;15:3845-3852
16 Li B, Dou QP. Bax degradation by the ubiquitin/ proteasome-dependent pathway: involvement in tumor survival and progression. Proc Natl Acad Sci USA 2000;97:3850-3855   DOI   ScienceOn
17 Martinez-Diez MC, Serrano MA, Monte MJ, Marin JJ. Comparison of the effects of bile acids on cell viability and DNA synthesis by rat hepatocytes in primary culture. Biochim Biophys Acta 2000;1500: 153-160   DOI   ScienceOn
18 Orlowski RZ. The role of the ubiquitin-proteasome pathway in apoptosis. Cell Death Differ 1999;6: 303-313   DOI   ScienceOn
19 Susin SA, Lorenzo HK, Zamzami N et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-446   DOI   ScienceOn
20 Shen J, Huang C, Jiang L et al.. Enhancement of cisplatin induced apoptosis by suberoylanilide hydroxamic acid in human oral squamous cell carcinoma cell lines. Biochem Pharmacol 2007;73:1901-1909   DOI   ScienceOn
21 Golab J, Stoklosa T, Czajka A et al. Synergistic antitumor effects of a selective proteasome inhibitor and TNF in mice. Anticancer Res 2000;20:1717-1721
22 Orrenius S. Mitochondrial regulation of apoptotic cell death. Toxicol. Lett 2004;149:19-23   DOI
23 Green DR, Reed JC. Mitochondria and apoptosis. Science 1998;281:1309-1312   DOI
24 Seo SY, Jun EJ, Jung SM et al. Synthetic chenodeoxycholic acid derivative HS-1200-induced apoptosis of p815 mastocytoma cells is augmented by co-treatment with lactacystin. Anticancer Drugs 2003;14:219-225   DOI   ScienceOn
25 Choi HJ, Kim HH, Lee HS et al. Lactacystin augments the sulindac-induced apoptosis in HT-29 cells. Apoptosis 2003;8:301-305   DOI   ScienceOn
26 Rust C, Karnitz LM, Paya CV, Moscat J, Simari RD, Gores GJ. The bile acid taurochenodeoxycholate activates a phosphatidylinositol 3-kinase-dependent survival signaling cascade. J Biol Chem 2000;275: 20210-20216   DOI   ScienceOn
27 Orlowski RZ, Eswara JR, Lafond-Walker A, Grever MR, Orlowski M, Dang CV. Tumor growth inhibition induced in a murine model of human Burkitt's lymphoma by a proteasome inhibitor. Cancer Res 1998;58:4342-4348
28 Li P, Nijhawan D, Budihardjo I et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-489   DOI   ScienceOn
29 Daugas E, Susin SA, Zamzami N et al. Mitochondrio -nuclear translocation of AIF in apoptosis and necrosis. FASEB J 2000;14:729-739   DOI
30 Choi Y H, Im EO, Suh H et al. Apoptotic activity of novel bile acid derivatives in human leukemic T cells through the activation of caspases. Int J Oncol 2001;18:979-984
31 Martinez JD, Stratagoules ED, LaRue JM et al. Different bile acids exhibit distinct biological effects: the tumor promoter deoxycholic acid induces apoptosis and the chemopreventive agent ursodeoxycholic acid inhibits cell proliferation. Nutr Cancer 1998;31:111-118   DOI   ScienceOn
32 Wagenknecht B, Hermisson M, Groscurth P, Liston P, Krammer PH, Weller M. Proteasome inhibitorinduced apoptosis of glioma cells involves the processing of multiple caspases and cytochrome c release. J Neurochem 2000;75:2288-2297   DOI   ScienceOn
33 Patel T, Roberts LR, Jones BA, Gores GJ. Dysregulation of apoptosis as a mechanism of liver disease: an overview. Semin Liver Dis 1998;18:105-114   DOI   ScienceOn
34 Grimm LM, Goldberg AL, Poirier GG, Schwartz LM, Osborne BA. Proteasomes play an essential role in thymocyte apoptosis. EMBO J 1996;15:3835-3844
35 Hofmann AF. Chemistry and enterohepatic circulation of bile acids. Hepatology 1984;4(5 Suppl):4S-14S   DOI
36 Delic J, Masdehors P, Omura S et al. The proteasome inhibitor lactacystin induces apoptosis and sensitizes chemo- and radioresistant human chronic lymphocytic leukaemia lymphocytes to TNF-alphainitiated apoptosis. Br J Cancer 1998;77:1103-1107   DOI   ScienceOn
37 Fanelli M, Minucci S, Gelmetti V et al. Constitutive degradation of PML/RARalpha through the proteasome pathway mediates retinoic acid resistance. Blood 1999;93:1477-1481
38 Holtzman MJ, Green JM, Jayaraman S, Arch RH. Regulation of T cell apoptosis. Apoptosis 2000;5:459-471   DOI   ScienceOn
39 Chandra J, Niemer I, Gilbreath J et al. Proteasome inhibitors induce apoptosis in glucocorticoid-resistant chronic lymphocytic leukemic lymphocytes. Blood 1998;92: 4220-4229
40 Liu X, Li P, Widlak P et al. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-8466   DOI
41 Marshansky V, Wang X, Bertrand R et al. Proteasomes modulate balance among proapoptotic and antiapoptotic Bcl-2 family members and compromise functioning of the electron transport chain in leukemic cells. J Immunol 2001;166:3130-3142   DOI
42 Im EO, Choi YH, Paik KJ et al. Novel bile acid derivatives induce apoptosis via a p53-independent pathway in human breast carcinoma cells. Cancer Lett 2001;163:83-93   DOI   ScienceOn
43 Kankaanranta H, Giembycz MA, Barnes PJ, el- Haddad B, Saarelainen S, Zhang X, Moilanen E, Lindsay MA. Hydrogen peroxide reverses IL-5 afforded eosinophil survival and promotes constitutive human eosinophil apoptosis. Int Arch Allergy Immunol 2002;127:73-78   DOI   ScienceOn
44 Yoon HS, Rho JH, Yoo KW et al. Synthetic bile acid derivatives induce nonapoptotic death of human retinal pigment epithelial cells. Curr Eye Res 2001;22:367-374   DOI   ScienceOn