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http://dx.doi.org/10.5536/KJPS.2020.47.3.169

Effects of Probiotic Complex on Performance, Blood Biochemical and Immune Parameters, Digestive Enzyme Activity, Fecal Microbial Population and Noxious Gas Emission in Broiler Chicks  

Kim, Min-Jeong (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Jeon, Dong-Gyung (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Ahn, Ho-Sung (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Yoon, Il-Gyu (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Moon, Eun-Seo (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Lee, Chai-Hyun (Technical Research Team, Hafeed, Korea Industrial C., Ltd)
Lim, Yong (Department of Clinical Laboratory Science, Dong-Eui Univerisity)
Jang, In-Surk (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Publication Information
Korean Journal of Poultry Science / v.47, no.3, 2020 , pp. 169-180 More about this Journal
Abstract
This study examined the effects of a probiotic complex (PC) containing Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae on growth performance, organ weight, immune parameters, fecal microbial count, and noxious odor in broiler chicks. A total of 216 birds (4-day-old) were fed a basal diet (CON) and a diet supplemented with 0.25% (PC1) and 0.5% (PC2) of PC until 35 days of age. No difference in body weight, feed intake, and FCR was observed among the groups. The intestinal mucosal weight of the PC1 group was greater than that of the CON group without affecting weights of the other organs. Intestinal secretory immunoglobulin A (sIgA) levels in the PC2 group increased significantly (P<0.05) compared with that in the CON group. The PC2 group also had a strong tendency for elevated blood sIgA levels. Dietary PC did not affect the level of interleukin-1β in the blood and mucosal tissues or alter maltase, sucrase, and leucine aminopeptidase activities in the intestinal mucosa. The PC2 group had higher colony-forming units (cfu) for L. plantarum and S. cerevisiae, but lower cfu for E. coli than those in the CON group. Compared to the CON diet, the PC2 diet resulted in a decreased H2S concentration and a tendency toward decreased CH3SH concentration. In conclusion, a 0.5% PC diet showed increased sIgA and desirable microbial population, and decreased noxious odor in the feces, suggesting that PC could be applied as an environmentally friendly feed additive in broiler chicks.
Keywords
probiotic complex; performance; immune parameters; microbial count; noxious gas;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Aktas B, De Wolfe TJ, Safdar N, Darien BJ, Steele JL 2016 The impact of Lactobacillus casei on the composition of the cecal microbiota and innate immune system is strain specific. PLoS One 11(5):e0156374.   DOI
2 Awad WA, Ghareeb K, Abdel-Raheem S, Bohm J 2009 Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult Sci 88(1):49-56.   DOI
3 Bai SP, Wu AM, Ding XM, Lei Y, Bai J, Zhang KY, Chio JS 2013 Effects of probiotic supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poult Sci 92(3):663-670.   DOI
4 Chen KL, Kho WL, You SH, Yeh RH, Tang SW, Hsieh CW 2009 Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae mixed fermented feed on the enhanced growth performance of broilers. Poult Sci 88(2):309-315.   DOI
5 Chu GM, Lee S, Jeong HS, Lee SS 2011 Efficacy of probiotics from anaerobic microflora with prebiotics on growth performance and noxious gas emission in growing pigs. Anim Sci J 82(2):282-290.   DOI
6 Collington GK, Parker DS, Armstrong DG 1990 The influence of inclusion of either an antibiotic and a probiotic in the diet on the development of digestive enzyme activity in the pig. Br J Nutr 64:59-70.   DOI
7 Dahlgvist A 1968 Assay of the intestinal disaccharidase. Anal Biochem 22:99-107.   DOI
8 Fukushima M, Nakano M 1995 The effect of a probiotic on fecal and liver lipid classes in rats. Br J Nutr 73(5):701-710.   DOI
9 Fuller R 1989 Probiotics in man and animals: a review. J Appl Bacteriol 66:365-378.   DOI
10 Gadde UD, Oh S, Lee Y, Davis E, Zimmerman N, Rehberger T, Lillehoj HS 2017 Dietary Bacillus subtilis-based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens. Res Vet Sci 114:236-243.   DOI
11 Gal-Garber O, Uni Z 2000 Chicken intestinal aminopeptidase: partial sequence of the gene, expression and activity. Poult Sci 79(1):41-45.   DOI
12 Giannini E, Botta F, Fasoli A, Ceppa P, Risso D, Lantieri PB, Celle G, Testa R 1999 Progressive liver functional impairment is associated with an increase in AST/ALT ratio. Dig Dis Sci 44(6):1249-1253.   DOI
13 Hosoi T, Ametani A, Kiuchi K, Kaminogawa S 1999 Changes in fecal microflora induced by intubation of mice Bacillus subtilis (Natto) spores are dependent upon dietary components. Can J Microbiol 45(1):59-66.   DOI
14 Kabir SM 2009 The role of probiotics in the poultry industry. Int J Mol Sci 10:3531-3546.   DOI
15 Huang MK, Choi YJ, Houde R, Lee JW, Lee B, Zhao X 2004 Effects of Lactobacilli and an acidophilic fungus on the production performance and immune responses in broiler chickens. Poult Sci 83(5):788-795.   DOI
16 Huyghebaert G, Ducatelle R, Van Immerseel F 2011 An update on alternatives to antimicrobial growth promoters for broilers. Vet J 187(2):182-188.   DOI
17 Jin LZ, Ho YW, Abdullah N, Jalaludin S 1998 Growth performance, intestinal microbial population, and serum cholesterol of broilers fed diets containing Lactobacillus culture. Poult Sci 77:1259-1265.   DOI
18 Kim JS, Ingale SL, Kim YW, Kim KH, Sen S, Ryu MH, Lohakare JD, Kwon IK, Chae BJ 2012 Effect of supplementation of multi-microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers. J Anim Physiol Anim Nutr (Berl) 96(4):618-626.   DOI
19 Kim YR, Ahn BK, Kim MS, Kang CW 2010 Effects of dietary supplementation of probiotics (MS102$^{(R)}$) on performance, blood cholesterol level, size of small intestine and intestinal microflora in broiler chicks. Kor J Anim Sci 42(6):849-858.
20 Ko YD, Sin JH, Kim SC, Kim YM, Park KD, Kim JH 2003 Effects of dietary probiotics on performance, noxious gas emission and microflora population on the cecum in broiler. J Anim Sci Technol 45(4):559-568.   DOI
21 Lan R, Tran H, Kim I 2017 Effects of probiotic supplementation in different nutrient density diets on growth performance, nutrient digestibility, blood profiles, fecal microflora and noxious gas emission in weaning pig. J Sci Food Agric 97(4):1335-1341.   DOI
22 Ng SC, Hart AL, Kamm MA, Stagg AJ, Knight SC 2009 Mechanisms of action of probiotics: recent advances. Inflamm Bowel Dis 15(2):300-310.   DOI
23 Lee KW, Lillehoj HS, Jang SI, Lee SH 2014 Effects of salinomycin and Bacillus subtilis on growth performance and immune responses in broiler chickens. Res Vet Sci 97(2):304-308.   DOI
24 Li HL, Li ZJ, Wei ZS, Liu T, Zou XZ, Liao Y, Luo Y 2015 Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers. J Zhejiang Univ Sci B 16(12):1019-1026.   DOI
25 Nava GM, Bielke LR, Callaway TR, Castaneda MP 2005 Probiotic alternatives to reduce gastrointestinal infections: the poultry experience. Anim Health Res Rev 6(1):105-118.   DOI
26 Nurmi E, Rantala M 1973 New aspects of Salmonella infection in broiler production. Nature 241:210.   DOI
27 O'Shea CJ, Sweeney T, Bahar B, Ryan MT, Thornton K, O'Doherty JV 2012 Indices of gastrointestinal fermentation and manure emissions of growing-finishing pigs as influenced through singular or combined consumption of Lactobacillus plantarum and inulin. J Anim Sci 90(11):3848-3857.   DOI
28 Park SH, Choi JS, Jung DS, Auh JH, Choi YI 2010 Effects of complex probiotics and antibiotics on growth performance and meat quality in broilers. Kor J Food Sci Ani Resour 30(3):504-511.   DOI
29 Pourakbari M, Seidavi A, Asadpour L, Martinez A 2016 Probiotic level effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers. An Acad Bras Cienc 88(2):1011-1021.   DOI
30 Rajput IR, Li LY, Xin X, Wu BB, Juan ZL, Cui ZW, Yu DY, Li WF 2013 Effect of Saccharomyces boulardii and Bacillus subtilis B10 on intestinal ultrastructure modulation and mucosal immunity development mechanism in broiler chickens. Poult Sci 92(4):956-965.   DOI
31 Rodjan P, Soisuwan K, Thongprajukaew K, Theapparat Y, Khongthong S, Jeenkeawpieam J, Salaeharae T 2018 Effect of organic acids or probiotics alone or in combination on growth performance, nutrient digestibility, enzyme activities, intestinal morphology and gut microflora in broiler chickens. J Anim Physiol Anim Nutr (Berl) 102(2):e931-e940.   DOI
32 Shang QH, Yang ZB, Yang WR, Li Z, Zhang GG, Jiang SZ 2016 Toxicity of mycotoxins from contaminated corn with or without yeast cell wall adsorbent on broiler chickens. Asian-Aust J Anim Sci 29(5):674-680.   DOI
33 Shokryazdan P, Faseleh Jahromi M, Liang JB, Ramasamy K, Sieo CC, Ho YW 2017 Effects of a Lactobacillus salivarius mixture on performance, intestinal health and serum lipids of broiler chickens. PLoS One 12(5):e0175959.   DOI
34 Wang X, Farnell YZ, Peebles ED, Kiess AS, Wamsley KG, Zhai W 2016 Effects of prebiotics, probiotics, and their combination on growth performance, small intestine morphology, and resident Lactobacillus of male broilers. Poult Sci 95(6):1332-1340.   DOI
35 Wilkins T, Sequoia J 2017 Probiotics for gastrointestinal conditions: a summary of the evidence. Am Fam Physician 96(3):170-178.
36 Yeo J, Kim K 1997 Effects of feeding diets containing an antibiotic, a probiotic, or yucca extract on growth and intestinal urease activity in broiler chicks. Poult Sci 76:381-385.   DOI
37 Ahmed ST, Islam M, Mun HS, Sim HJ, Kim YJ, Yang CJ 2014 Effects of Bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broiler chickens. Poult Sci 93(8):1963-1971.   DOI
38 Zhang ZF, Kim IH 2013 Effects of probiotic supplementation in different energy and nutrient density diets on performance, egg quality, excreta microflora, excreta noxious gas emission, and serum cholesterol concentrations in laying hens. J Anim Sci 91(10):4781-4787.   DOI
39 Abboud G, Kaplowitz N 2007 Drug-induced liver injury. A review. Drug Saf 30(4):277-294.   DOI