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http://dx.doi.org/10.5536/KJPS.2019.46.4.287

Identification of Female Specific Genes in the W Chromosome that are Expressed during Gonadal Differentiation in the Chicken  

Rallabandi, Harikrishna Reddy (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Jo, Yong Jin (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Lee, Hwi Cheul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Byun, Sung June (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Lee, Bo Ram (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Korean Journal of Poultry Science / v.46, no.4, 2019 , pp. 287-296 More about this Journal
Abstract
Avian sex determination system involves the male ZZ and female ZW chromosomes. However, very few studies are reported the expression, functional role and importance of genes on the W chromosome because of its small and highly heterochromatic genomic regions. Recent studies demonstrated that the W chromosome may have critical roles in physiology, sex determination and subsequent sexual differentiation in chickens. Therefore, gene annotation, including describing the expression and function of genes in the chicken W chromosome, is needed. In this study, we have searched the W chromosome of chickens and selected a total of 36 genes to evaluated their specific expression in the testis and ovary at various developmental stages such as embryonic day 6 (E6), hatch and adult. Interestingly, out of 36 genes in chicken W chromosome, we have found seven female-specific expression at E6.5 day, indicating that they are functionally related to female chicken gonadal differentiation. In addition, we have identified the stage specific gene expression from the sex specific genes. Furthermore, we analyzed the relative location of genes in the chicken W chromosome. Collectively, these results will contribute molecular insights into the sexual determination, differentiation and female development based on the W chromosome.
Keywords
chicken; W chromosome; gonadal differentiation; female specific expression;
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1 Ellergen Hans 1996 First gene on the avian W chromosome (CHD) provides a tag for universal sexing of non-ratite birds. Proceedings of the Royal Society B263(1377):1635-1641.
2 Fridolfsson AK, Cheng H, Copeland NG, Jenkins NA, Liu HC, Raudsepp T, Woodage T, Chowdhary B, Halverson J, Ellegren H 1998 Evolution of the avian sex chromosomes from an ancestral pair of autosomes. Proceedings of the National Academy of Sciences of the United States of America 95(14):8147-8152.   DOI
3 Graves JAM 2014 Avian sex, sex chromosomes, and dosage compensation in the age of genomics. Chromosome Research 22(1):45-57.   DOI
4 Hamburger V, Hamilton HL 1951 A series of normal stages in the development of the chick embryo. J Morphol 88(1):49-92.   DOI
5 Kiuchi T, Koga H, Kawamoto M, Shoji K, Sakai H, Arai Y, Ishihara G, Kawaoka S, Sugano S, Shimada T, Suzuki Y, Suzuki MG, Katsuma S 2014 A single female-specific piRNA is the primary determiner of sex in the silkworm. Nature 509(7502):633-636.   DOI
6 Lee BR, Kim H, Park TS, Moon S, Cho S, Park T, Lim JM, Han JY 2007 A set of stage-specific gene transcripts identified in EK stage X and HH stage 3 chick embryos. BMC Developmental Biology 7(1):60.   DOI
7 Mank JE 2012 Small but mighty: the evolutionary dynamics of W and Y sex chromosomes. Chromosome Research 20(1):21-33.   DOI
8 Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG 2012 Primer3--new capabilities and interfaces. Nucleic Acids Res 40(15):e115.   DOI
9 Smith CA, Roeszler KN, Ohnesorg T, Cummins DM, Farlie PG, Doran TJ, Sinclair AH 2009 The avian Z-linked gene DMRT1 is required for male sex determination in the chicken. Nature 461(7261):267-271.   DOI
10 Rengaraj D, Lee BR, Lee SI, Seo HW, Han JY 2011 Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells. Plos One 6(5):e19524.   DOI
11 Smith CA, Sinclair AH 2004 Sex determination: insights from the chicken. Bioessays 26(2):120-132.   DOI
12 Solari AJ, Fechheimer NS, Bitgood JJ 1988 Pairing of ZW gonosomes and the localized recombination nodule in two Z-autosome translocations in Gallus domesticus. Cytogenet 48(3):130-136.   DOI
13 Ayers KL, Sinclair AH, Smith CA 2013b The molecular genetics of ovarian differentiation in the avian model. Sex Dev 7(1-3):80-94.   DOI
14 Matsubara K, Tarui H, Toriba M, Yamada K, Nishida-Umehara C, Agata K, Matsuda Y 2006 Evidence for different origin of sex chromosomes in snakes, birds, and mammals and step-wise differentiation of snake sex chromosomes. Proceedings of the National Academy of Sciences 103(48):18190-18195.   DOI
15 Moghadam HK, Pointer MA, Wright AE, Berlin S, Mank JE 2012 W chromosome expression responds to female-specific selection. Proceedings of the National Academy of Sciences of the United States of America 109(21):8207-8211.   DOI
16 Ayers KL, Davidson NM, Demiyah D, Roeszler KN, Grutzner F, Sinclair AH, Oshlack A, Smith CA 2013a RNA sequencing reveals sexually dimorphic gene expression before gonadal differentiation in chicken and allows comprehensive annotation of the W-chromosome. Genome Biology 14(3):R26.   DOI
17 Bachtrog D, Kirkpatrick M, Mank JE, McDaniel SF, Pires JC, Rice WR, Valenzuela N 2011 Are all sex chromosomes created equal? Trends in Genetics 27(9):350-357.   DOI
18 Berlin S, Tomaras D, Charlesworth B 2007 Low mitochondrial variability in birds may indicate Hill-Robertson effects on the W chromosome. Heredity 99(4):389-396.   DOI
19 Chue J, Smith CA 2011 Sex determination and sexual differentiation in the avian model. FEBS J 278(7):1027-1034.   DOI