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http://dx.doi.org/10.5536/KJPS.2019.46.3.161

Effects of Dietary Zinc Supplements on the Antioxidant Indicators and the Expression of Zinc Transport Genes in Korean Native Chicks  

Jeon, Dong-Gyung (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Kim, Min-Jeong (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Yoon, Il-Gyu (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Ahn, Ho-Sung (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Sohn, Sea-Hwan (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Jang, In-Surk (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
Publication Information
Korean Journal of Poultry Science / v.46, no.3, 2019 , pp. 161-171 More about this Journal
Abstract
Four-week-old male Korean native chicks (KNC) were assigned to 3 groups with 6 replicates (8 birds/replicate) in each group: a basal diet (CON, 100 ppm of Zn), basal diet fortified with 50 ppm of Zn with zinc oxide (ZnO), or basal diet fortified with 50 ppm of Zn with Zn-methionine (ZnM). Immediately after a 4-week-feeding trial, 6 birds per group were used to evaluate the effects of zinc supplements on antioxidant indicators and the mRNA expression of zinc transport genes. The nitrogen components, lipid peroxidation, and total antioxidant status in blood were not influenced by Zn fortified diets. However, the ZnM group showed a significant (P<0.05) increase in uric acid levels than those in the ZnO group. In the small intestine, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities, and malondialdehyde (MDA) level were unaffected by zinc supplements. The activity of glutathione S-transferase (GST) was significantly (P<0.05) enhanced by Zn-methionine supplementation. In the liver, the activity of GST was significantly (P<0.05) increased by Zn-methionine supplement without affecting SOD, GPX, and MDA levels. With respect to the mRNA expression of zinc transport genes, the ZnM group displayed a strong tendency for increases in intestinal ZnT-1 (P=0.09) and ZnT-5 (P=0.06) levels, compared to those in the CON group. Moreover, the ZnM group showed a tendency (P=0.10) for up-regulation of hepatic metallothionein mRNA as compared with the CON group. In conclusion, the Zn-fortified diet with 50 ppm of Zn-methionine helped to improve GST activity and Zn transport gene expression in the small intestine or liver of KNC.
Keywords
zinc oxide; Zn-methionine; antioxidant enzymes; Zn transport genes;
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1 Abd El-Wahab A, Radko D, Kamphues J 2013 High dietary levels of biotin and zinc to improve health of foot pads in broilers exposed experimentally to litter with critical moisture content. Poult Sci 92(7):1774-1782.   DOI
2 Alam S, Kelleher SL 2012 Cellular mechanisms of zinc dysregulation: a perspective on zinc homeostasis as an etiological factor in the development and progression of breast cancer. Nutrients 4(8):875-903.   DOI
3 Bidlack WR, Tappel AL 1973 Damage to microsomal membrane by lipid peroxidation. Lipid 8(4):177-182.   DOI
4 Bun SD, Guo YM, Guo FC, Ji FJ, Cao H 2011 Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella. Poult Sci 90(8):1220-1226.   DOI
5 Cabre M, Camps J, Ferre N, Paternain JL, Joven J 2001 The antioxidant and hepatoprotective effects of zinc are related to hepatic cytochrome P450 depression and metallothionein induction in rats with experimental cirrhosis. Int J Vitam Nutr Res 71(4):229-236.   DOI
6 Cragg RA, Phillips SR, Piper JM, Varma JS, Campbell FC, Mathers JC, Ford D 2005 Homeostatic regulation of zinc transporters in the human small intestine by dietary zinc supplementation. Gut 54(4):469-478.   DOI
7 Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sanchez-Perez P, Cadenas S, Lamas S 2015 Antioxidant responses and cellular adjustments to oxidative stress. Redox Biol 6:183-197.   DOI
8 Habig WH, Pabst MJ, Jakoby WB 1974 Glutathionine S transferase: the first enzymatic steps in mercapturic acid formation. J Biol Chem 249(22):7130-7139.   DOI
9 Halliwell B, Gutteridge JM 1990 The antioxidants of human extracellular fluids. Arch Biochem Biophys 280(1):1-8.   DOI
10 Hayes JD, Flanagan JU, Jowsey IR 2005 Glutathione transferases. Annu Rev Pharmacol Toxicol 45:51-88.   DOI
11 He B, Bortoluzzi C, King WD, Graugnard D, Dawson KA, Applegate TJ 2019 Zinc source influences the gene expression of zinc transporters in jejunum and cecal tonsils during broiler challenge with Eimeria maxima and Clostridium perfringens. Poult Sci 98(3):1146-1152.   DOI
12 Huang YL, Lu L, Luo XG, Liu B 2007 An optimal dietary zinc level of broiler chicks fed a corn-soybean meal diet. Poult Sci 86(12):2582-2589.   DOI
13 Jamakala O, Rani UA 2015 Amelioration effect of zinc and iron supplementation on selected oxidative stress enzymes in liver and kidney of cadmium-treated male albino rat. Toxicol Int 22(1):1-9.   DOI
14 Kimura T, Kambe T 2016 The functions of metallothionein and ZIP and ZnT transporters: an overview and perspective. Int J Mol Sci 17(3):336-358.   DOI
15 Korea Feeding Standard for Poultry 2007 National Institute of Animal Science, Rural Development Administration.
16 Li L, Li P, Chen Y, Wen C, Zhuang S, Zhou Y 2015 Zinc-bearing zeolite clinoptilolite improves tissue zinc accumulation in laying hens by enhancing zinc transporter gene mRNA abundance. Anim Sci J 86(8):782-789.   DOI
17 Liochev SI, Fridovich I 2000 Cu- and Zn-containing superoxide dismutase can act as a superoxide reductase and a superoxide oxidase. J Biol Chem 275(49):38482-38485.   DOI
18 Mansour SA, Abbassy MA, Shaldam HA 2017 Zinc ameliorate oxidative stress and hormonal disturbance induced by methomyl, abamectin, and their mixture in male rats. Toxics 5(4):E37-54.   DOI
19 Liu ZH, Lu L, Wang RL, Lei HL, Li SF, Zhang LY, Luo XG 2015 Effects of supplemental zinc source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Poult Sci 94(11):2686-2694.   DOI
20 Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2$^{-{\Delta}{\Delta}CT}$ method. Methods 25(4):402-408.   DOI
21 McMahon RJ, Cousins RJ 1998 Mammalian zinc transporters. J Nutr 128(4):667-670.   DOI
22 National Research Council 1994 Nutrient Requirement of Poultry. 9th rev ed. National Academic Press. Washington, DC, USA.
23 Nishito Y, Kambe T. 2018. Absorption mechanisms of iron, copper, and zinc: an overview. J Nutr Sci Vitaminol (Tokyo) 64(1):1-7   DOI
24 Park JH, Kweon GR 2013 Clinical applications of antioxidants. Hanyang Med Rev 33(2):130-136.   DOI
25 Prasad AS 2014 Zinc: an antioxidant and anti-inflammatory agent: role of zinc in degenerative disorders of aging. J Trace Elem Med Biol 28(4):364-371.   DOI
26 Rao SV, Prakash B, Raju MV, Panda AK, Kumari RK, Reddy EP 2016 Effect of supplementing organic forms of zinc, selenium and chromium on performance, antioxidant and immune responses in broiler chicken reared in tropical summer. Biol Trace Elem Res 172(2):511-520.   DOI
27 Ruttkay-Nedecky B, Nejdl L, Gumulec J, Zitka O, Masarik M, Eckschlager T, Stiborova M, Adam V, Kizek R 2013 The role of metallothionein in oxidative stress. Int J Mol Sci 14(3):6044-6066.   DOI
28 Surai PF 2003 Natural antioxidant in avian nutrition and reproduction. Pages 1-25 In: Antioxidant Systems in the Animal Body. Nottingham University Press, Thrumpton.
29 SAS 1996 User's Guide: Statistics Version 6.12 Ed. SAS Inst., Inc., Cary, NC.
30 Song Y, Leonard SW, Traber MG, Ho E 2009 Zinc deficiency affects DNA damage, oxidative stress, antioxidant defenses, and DNA repair in rats. J Nutr 139(9):1626-1631.   DOI
31 Navidshad B, Jabbari S, Mirzael Aghjeh Gheshlagh F 2016 The new progresses in Zn requirements of poultry. Iranian J App Anim Sci 6(4):763-767.
32 Tang Z, Wen C, Li P, Wang T, Zhou Y 2014 Effect of zinc-bearing zeolite clinoptilolite on growth performance, nutrient retention, digestive enzyme activities, and intestinal function of broiler chickens. Biol Trace Elem Res 158(1):51-57.   DOI
33 Tappel AL 1978 Glutathione peroxidase and hydroperoxides. Methods in Enzymol 52:506-513.   DOI
34 Tate DJ Jr, Miceli MV, Newsome DA 1999 Zinc protects against oxidative damage in cultured human retinal pigment epithelial cells. Free Radic Biol Med 26(5-6): 704-713.   DOI
35 Tupe RS, Tupe SG, Tarwadi KV, Agte VV 2010 Effect of different dietary zinc levels on hepatic antioxidant and micronutrients indices under oxidative stress conditions. Metabolism 59(11):1603-1611.   DOI
36 Wang ZC, Yu HM, Xie JJ, Cui H, Nie H, Zhang TT, Gao XH 2019 Effect of dietary zinc pectin oligosaccharides chelate on growth performance, enzyme activities, Zn accumulation, metallothionein concentration, and gene expression of Zn transporters in broiler chickens. J Anim Sci 97(5):2114-2124.   DOI
37 Zhao CY, Tan SX, Xiao XY, Qiu XS, Pan JQ, Tang ZX 2014 Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers. Biol Trace Elem Res 160(3):361-367.   DOI
38 Wayner DD, Burton GW, Ingold KU, Barclay LR, Locke SJ 1987 The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta 924(3):408-419.   DOI
39 Yang W, Chen Y, Cheng Y, Wen C, Zhou Y 2017 Effects of zinc bearing palygorskite supplementation on the growth performance, hepatic mineral content, and antioxidant status of broilers at early age. Asian-Aust J Anim Sci 30(7):1006-1012.   DOI
40 Yu Y, Lu L, Luo XG, Liu B 2008 Kinetics of zinc absorption by in situ ligated intestinal loops of broilers involved in zinc transporters. Poult Sci 87(6):1146-1155.   DOI
41 Zhou Z, Wang L, Song Z, Saari JT, McClain CJ, Kang YJ 2005 Zinc supplementation prevents alcoholic liver injury in mice through attenuation of oxidative stress. Am J Pathol 166(6):1681-1690.   DOI
42 Hamer DH 1986 Metallothionein. Annu Rev Biochem 55:913-951.   DOI
43 Kupfer D, Levin E 1972 Monooxygenase drug metabolizing activity in $CaCl_2$-aggregated hepatic microsomes from rat liver. Biochem Biophys Res Commun 47(3):611-618.   DOI