Browse > Article
http://dx.doi.org/10.5536/KJPS.2019.46.2.117

Effects of Dietary Zinc Supplements on Growth, Feed Efficiency, Organ Weight, Blood Biochemical Profiles, and Activity of Digestive Enzymes in Growing Korean Native Chicks  

Jeon, Dong-Gyung (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Kim, Min-Jeong (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Yoon, Il-Gyu (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Ahn, Ho-Sung (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Moon, Eun-Seo (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Sohn, Sea-Hwan (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Lim, Yong (Department of Clinical Laboratory Science, Dong-Eui Univerisity)
Jang, In-Surk (Department of Animal science & Biotechnology, Gyeongnam National University of Science and Technology)
Publication Information
Korean Journal of Poultry Science / v.46, no.2, 2019 , pp. 117-125 More about this Journal
Abstract
The aim of the study was to investigate the effects of dietary supplementation of zinc (Zn) sources (zinc oxide and Zn-methionine) on performance, organ weights, blood biochemical profiles, and digestive enzymes of the pancreas and small intestine in Korean native chicks (KNC). A total of 144 KNC (n=6) were fed a basal diet (CON, 100 ppm of Zn), a basal diet supplemented with 50 ppm of Zn with ZnO (ZNO), or a basal diet supplemented with 50 ppm of Zn with Zn-methionine (ZMT) for 28 days. There was no significant difference in body weight, gain, feed intake, and feed conversion ratio among the three groups. The relative weights of the liver, spleen, and intestinal mucosa were unaffected by the dietary source of Zn, whereas pancreas weight in the ZNO group decreased (P<0.05) compared with that in the CON and ZMT groups. Blood biochemical components including aspartate aminotransferase, and alanine aminotransferase were unaffected by dietary Zn supplementation. Pancreatic trypsin activity in the ZNO and ZMT groups was significantly (P<0.05) enhanced compared with that in the CON group. However, the activities of ${\alpha}$-amylase and carboxypeptidase A were not altered by dietary Zn supplementation. The activities of maltase and sucrase were unchanged, whereas the activity of leucine aminopeptidase tended (P=0.08) to be increased by dietary Zn supplementation. In conclusion, the supplementation with 50 ppm of ZnO or Zn-methionine resulted in an activation of protein digestive enzymes in the pancreas and small intestine without affecting animal performance in KNC.
Keywords
Korean native chicks; zinc oxide; Zn-methionine; digestive enzymes; pancreas and intestine;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brugger D, Windisch WM 2016 Subclinical zinc deficiency impairs pancreatic digestive enzyme activity and digestive capacity of weaned piglets. Br J Nutr 116(3):425-433.   DOI
2 Bun SD, Guo YM, Guo FC, Ji FJ, Cao H 2011 Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella. Poult Sci 90(6):1220-1226.   DOI
3 Dahlgvist A 1968 Assay of the intestinal disaccharidase. Anal Biochem 22(1):99-107.   DOI
4 Folk JE, Schirmer EW 1963 The porcine pancreatic carboxypeptidase A system I. Three forms of the active enzymes. J Biol Chem 238:3884-3894.   DOI
5 Gal-Garber O, Uni Z 2000 Chicken intestinal aminopeptidase: partial sequence of the gene, expression and activity. Poult Sci 79(1):41-45.   DOI
6 Geiger R, Fritz H 1986 Trypsin. Vo l5. Pages 119-128 In: Methods of Enzymatic Analysis. Bergmeyer H. Academic Press. New York.
7 Ghiasi Ghalehkandi J, Karamouz H, Zadeh Adam Nazhad H, Maheri Sis N, Beheshti R 2011 Effect of different levels of zinc cxide supplement on leucine aminopeptidase enzyme activity in small intestine of male broiler chicks 2011. Int J Anim Vet Advances 3(5):313-315.
8 Abd El-Wahab A, Radko D, Kamphues J 2013 High dietary levels of biotin and zinc to improve health of foot pads in broilers exposed experimentally to litter with critical moisture content. Poult Sci 92(7):1774-1782.   DOI
9 Abedini M, Shariatmadari F, Karimi Torshizi MA, Ahmadi H 2018 Effects of zinc oxide nanoparticles on the egg quality, immune response, zinc retention, and blood parameters of laying hens in the late phase of production. J Anim Physiol Anim Nutr (Berl) 102(1):736-745.   DOI
10 Giannini E, Botta F, Fasoli A, Ceppa P, Risso D, Lantieri PB, Celle G, Testa R 1999 Progressive liver functional impairment is associated with an increase in AST/ALT ratio. Dig Dis Sci 44(6):1249-1253.   DOI
11 Hara T, Takeda TA, Takagishi T, Fukue K, Kambe T, Fukada T 2017 Physiological roles of zinc transporters: molecular and genetic importance in zinc homeostasis. J Physiol Sci 67(2):283-301.   DOI
12 He B, Bortoluzzi C, King WD, Graugnard D, Dawson KA, Applegate TJ 2019 Zinc source influences the gene expression of zinc transporters in jejunum and cecal tonsils during broiler challenge with Eimeria maxima and Clostridium perfringens. Poult Sci 98(3):1146-1152.   DOI
13 Kimura T, Kambe T 2016 The functions of metallothionein and ZIP and ZnT transporters: An overview and perspective. Int J Mol Sci 17(3):336.   DOI
14 Bernfield P 1955 Amylases alpha and beta. Vol 1. pages 149-151. In: Methods in Enzymology. Colowick SP, Kaplan NO. Academic Press, New York.
15 Hedemann MS, Jensen BB, Poulsen HD 2006 Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs. J Anim Sci 84(12):3310-3320.   DOI
16 Hu CH, Qian ZC, Song J, Luan ZS, Zuo AY 2013 Effects of zinc oxide-montmorillonite hybrid on growth performance, intestinal structure, and function of broiler chicken. Poult Sci 92(1):143-150.   DOI
17 Huang YL, Lu L, Luo XG, Liu B 2007 An optimal dietary zinc level of broiler chicks fed a corn-soybean meal diet. Poult Sci 86(12):2582-2589.   DOI
18 Jing MY, Sun JY, Weng XY, Wang JF 2009 Effects of zinc levels on activities of gastrointestinal enzymes in growing rats. J Anim Physiol Anim Nutr (Berl) 93(5):606-612.   DOI
19 Kaya S, Kececi T, Haliloglu S 2001 Effects of zinc and vitamin A supplements on plasma levels of thyroid hormones, cholesterol, glucose and egg yolk cholesterol of laying hens. Res Vet Sci 71(2):135-139.   DOI
20 Kim HN, Jeon DG, Lee CY, Jang IS 2018 Effects of dietary lipid-coated zinc on the antioxidant defense system in the small intestine and liver of piglets. Lab Anim Res 34(2):65-74.   DOI
21 Korea Feeding Standard for Poultry 2007 National Institute of Animal Science, Rural Development Administration.
22 Liu ZH, Lu L, Wang RL, Lei HL, Li SF, Zhang LY, Luo XG 2015 Effects of supplemental zinc source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Poult Sci 94(11):2686-2694.   DOI
23 Lu J, Combs GF Jr 1988 Effect of excess dietary zinc on pancreatic exocrine function in the chick. J Nutr 118(6):681-689.   DOI
24 Sahin K, Sahin N, Kucuk O, Hayirli A, Prasad AS 2009 Role of dietary zinc in heat-stressed poultry: a review. Poult Sci 88(10):2176-2183.   DOI
25 Martin L, Pieper R, Schunter N, Vahjen W, Zentek J 2013 Performance, organ zinc concentration, jejunal brush border membrane enzyme activities and mRNA expression in piglets fed with different levels of dietary zinc. Arch Anim Nutr 67(3):248-261.   DOI
26 National Research Council 1994 Nutrient Requirement of Poultry. 9th rev ed. National Academic Press. Washington, DC, USA.
27 Park JH, Grandjean CJ, Antonson DL, Vanderhoof JA 1985 Effects of short-term isolated zinc deficiency on intestinal growth and activities of several brush border enzymes in weaning rats. Pediatr Res 19(12):1333-1336.   DOI
28 Prasad AS 1995 Zinc: an overview. Nutrition. 11(1 Suppl):93-99.
29 Prasad AS, Kucuk O 2002 Zinc in cancer prevention. Cancer Metastasis Rev 21(3-4):291-295.   DOI
30 Salim HM, Lee HR, Jo C, Lee SK, Lee BD 2012 Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens. Biol Trace Elem Res 147(1-3):120-129.   DOI
31 SAS 1996 User's Guide: Statistics Version 6.12 Ed. SAS Inst., Inc., Cary, NC.
32 Stahl JL, Cook ME, Sunde ML 1986 Zinc supplementation: Its effect on egg production, feed conversion, fertility, and hatchability. Poult Sci 65(11):2104-2109.   DOI
33 Tang Z, Wen C, Li P, Wang T, Zhou Y 2014 Effect of zinc-bearing zeolite clinoptilolite on growth performance, nutrient retention, digestive enzyme activities, and intestinal function of broiler chickens. Biol Trace Elem Res 158(1):51-57.   DOI
34 Zhang B, Shao Y, Liu D, Yin P, Guo Y, Yuan J 2012 Zinc prevents Salmonella enterica serovar Typhimurium induced loss of intestinal mucosal barrier function in broiler chickens. Avian Pathol 41(4):361-367.   DOI
35 Troche C, Eicher SD, Applegate TJ 2015 The influence of dietary zinc source and coccidial vaccine exposure on intracellular zinc homeostasis and immune status in broiler chickens. Br J Nutr 114(2):202-212.   DOI
36 Underwood E, Suttle NF 1990 The Mineral Nutrition of Livestock. CABI Publishing, New York.
37 Wen M, Zhao H, Liu G, Chen X, Wu B, Tian G, Cai J, Jia G 2018 Effect of zinc supplementation on growth performance, intestinal development, and intestinal barrier-related gene expression in pekin ducks. Biol Trace Elem Res 183(2):351-360.   DOI
38 Ying AJ, Shu XL, Gu WZ, Huang XM, Shuai XH, Yang LR, Jiang MZ 2011 Effect of zinc deficiency on intestinal mucosal morphology and digestive enzyme activity in growing rat. Zhonghua Er Ke Za Zhi 49(4):249-254.
39 Yu Y, Lu L, Li SF, Zhang LY, Luo XG 2017 Organic zinc absorption by the intestine of broilers in vivo. Br J Nutr 117(8):1086-1094.   DOI