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http://dx.doi.org/10.5536/KJPS.2017.44.1.19

Histological Change of Uterus Endometrium and Expression of the Eggshell-related Genes according to Hen Age  

Park, Ji Ae (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology)
Cho, Eun Jung (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology)
Park, Jung Yeon (Department of Clinical Pathology, Jinju Health College)
Sohn, Sea Hwan (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology)
Publication Information
Korean Journal of Poultry Science / v.44, no.1, 2017 , pp. 19-28 More about this Journal
Abstract
The eggshell is an intricate and highly ordered structure composed of multiple layers and a calcified matrix. The eggshell is formed at the uterine segment of the chicken oviduct. In this study, histological changes in the uterine endometrium and the expression of the eggshell-related genes were investigated according to hen age. We analyzed the expression of eggshell protein-related genes, such as OCX-32, OCX-36, OC-17, OC-116, and eggshell-ion-related genes, such as CABL-1, SPP1, SCNN1G, ATP2A2, CA2, and CALM1. In chicken uterine endometrium, histological deformation, fibrosis, atrophy and elimination of micro-villi were found with increasing hen age. The concentration of blood-ion components did not significantly change with age. The amount of telomeric DNA in uterine endometrial cells decreased with increasing hen age. The expression of most of the eggshell-related genes changed significantly with increasing hen age. The expression of some ovo-proteins, which play a role in eggshell formation, increased with increasing hen age; however, there were no significant correlations among eggshell protein genes. Eggshell ion-related genes, such as ATP2A2, SCNN1G, CA2, and CALM1, were closely related to each other. The OCX-32 and OCX-36 genes were closely related to some of the eggshell ion genes. Eggshell protein-related genes, such as the OCX-32, OCX-36 genes and ion-related genes such as CALB-1, ATP2A2, SCNN1G, CA2, CALM1, affected eggshell formation, mutually or independently. This study shows that, uterine although endometrial cell damage occurs with increasing hen age, normal eggshells can be formed in old hens. This suggests that eggshell protein-and eggshell ion-related genes also control the homeostasis of eggshell formation.
Keywords
aging; eggshell-related gene; uterus endometrium; egg shell; chicken;
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1 Huang CL, Sun L, Moonga BS, Zaidi M 2006 Molecular physiology and pharmacology of calcitonin. Cell Mol Biol 52:33-43.
2 Huntley DM, Holder DP 1978 Ultrastructure of shell gland tissue from hens producing good and poor eggshells. Poult Sci 57:1365-1368.   DOI
3 Ingleton PM 2002 Parathyroid hormone-related protein in lower vertebrates. Comp Biochem Physiol B 132B:87-95.
4 Jeong W, Lim W, Kim J, Ahn SE, Lee HC, Jeong JW, Han JY, Song G, Bazer FW 2012 Cell-specific and temporal aspects of gene expression in the chicken oviduct at different stages of the laying cycle. Biol Reprod 86(6):172, 1-8.
5 Jin YH, Lee KT, Lee WI, Han YK 2011 Effects of storage temperature and time on the quality of eggs from laying hens at peak production. Asian-Aust J Anim Sci 24:279-284.
6 Jonchere V, Brionne A, Gautron J, Nys Y 2012 Identification of uterine ion transporters for mineralisation precursors of the avian eggshell. BMC Physiology 12:10.   DOI
7 Kovacs-Nolan J, Cordeiro C, Young D, Mine Y, Hincke M 2014 Ovocalyxin-36 is an effector protein modulating the production of proinflammatory mediators. Vet Immun Immunopath 160:1-11.   DOI
8 Lavelin I, Meiri N, Einat M, Genina O, Pines M 2002 Mechanical strain regulation of the chicken glypican-4 gene expression in the avian eggshell gland. Am J Physiol Regul Integr Comp Physiol 283(4):R853-861.   DOI
9 Lee MH, Cho EJ, Choi ES, Sohn SH 2016 The effect of storage period and temperature on egg quality in commercial eggs. Kor J Poult Sci 43(1):31-38.   DOI
10 Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 25(4):402-408.   DOI
11 Mohammadpour AA, Zamanimoghadam A, Heidari M 2012 Comparative histomorphometrical study of genital tract in adult laying hen and duck. Vet Res Forum 3(1):27-30.
12 Peebles ED, Brake J 1987 Eggshell quality and hatchability in broiler breeder eggs. Poult Sci 66:596-604.   DOI
13 Pines M, Knopov V, Bar A 1994 Involvement of osteopontin in egg shell formation in the laying chicken. Matrix Biol 14:765-771.
14 Pooley AS 1979 Ultrastructural relationships of mineral and organic matter in avian eggshells. In Scanning Electron Microscopy/1979/II. SEM Inc. AMF O'Hare, IL.
15 Ramasamy I 2006 Recent advances in physiological calcium homeostasis. Clin Chem Lab Med 44:237-273.
16 Reyes-Grajeda JP, Moreno A, Romero A 2004 Crystal structure of ovocleidin-17, a major protein of the calcified Gallus gallus eggshell. JBC 279(39):40876-40881.   DOI
17 Roberson MS, Bliss SP, Xie J, Navratil AM, Farmerie TA, Wolfe MW, Clay CM 2005 Gonadotropin-releasing hormone induction of extracellular-signal regulated kinase is blocked by inhibition of calmodulin. Mol Endocrinol 19:2412-2423.   DOI
18 Roberts JR, Chousalkar K, Samiullah S 2013 Egg quality and age of laying hens: implications for product safety. Anim Prod Sci 53:1291-1297.
19 Roland DA Sr 1979 Factors influencing shell quality of aging hens. Poult Sci 58:774-777.   DOI
20 Romanoff AL 1949 The Avian Egg. John Wiley & Sons. Inc. New York, NY. pp. 353.
21 Samli HE, Agma A, Senkoylu N 2005 Effects of storage time and temperature on egg quality in old laying hens. J Appl Poult Res 14:548-553.   DOI
22 Silverman SL 2003 Calcitonin. Endocrinol Metab Clin N Am 32:273-284.   DOI
23 Soares JH Jr 1984 Calcium metabolism and its control- a review. Poult Sci 63:2075-2083.   DOI
24 Sohn SH, Subramani VK 2014 Dynamics of telomere length in the chicken. World's Poult Sci J 70:721-735.   DOI
25 Song J, Wolf SE, Wu XW, Finnerty CC, Gauglitz GG, Herndon DN, Jeschke MG 2009 Starvation induced proximal gut mucosal atrophy diminished with aging. J Parenter Enteral Nutr 33(4):411-416.   DOI
26 Suk YO, Park C 2001 Effect of breed and age of hens on the yolk to albumen ratio in two different genetic stocks. Poult Sci 80(7):855-858.   DOI
27 Sun YM, Dunn IC, Baines E, Talbot RT, Illing N, Millar RP, Sharp PJ 2001 Distribution and regulation by oestrogen of fully processed and variant transcripts of gonadotropin releasing hormone I and gonadotropin releasing hormone receptor mRNAs in the male chicken. J Neuroendocrinol 13:37-49.   DOI
28 Tyler 1969 A study of the eggshells of the Gaviiformes, Procellariiformes, Podicipitiformes and Pelecaniformes. J Zool London 158:395-412.
29 Uemoto Y, Suzuki C, Sato S, Sato S, Ohtake T, Sasaki O, Takahashi H, Kobayashi E 2009 Polymorphism of the ovocalyxin-32 gene and its association with egg production traits in the chicken. Poult Sci 88:2512-2517.   DOI
30 Walzem RL 1996 Lipoproteins and the laying hen: form follows function. Poult Avian Biol Rev 7:31-64.
31 Williams KC 1992 Some factors affecting albumen quality with particular reference to Haugh unit score. World's Poult Sci J 48:5-16.   DOI
32 Bar A, Vax E, Striem S 1999 Relationships among age, eggshell thickness and vitamin D metabolism and its expression in the laying hen. Comp Bioche Physiol 123:147-154.
33 Talmage RV, Mobley HT 2008 Calcium homeostasis: reassessment of the actions of parathyroid hormone. Gen Comp Endocrinol 156:1-8.   DOI
34 Akyurek H, Okur AA 2009 Effect of storage time, temperature and hen age on egg quality in free-range layer hens. J Anim Vet Adv 8:1953-1958.
35 Ariyadi B, Isobe N, Yoshimura Y 2012 Differences in the mucosal surface barrier formed by mucin in the lower oviductal segments between laying and molting hens. Poult Sci 91:1173-1178.   DOI
36 Bar A 2008 Calcium homeostasis, vitamin D metabolism and expression in strongly calcifying laying birds. Comp Biochem Physiol A 151:477-490.   DOI
37 Bar A 2009 Calcium transport in strongly calcifying laying birds: Mechanisms and regulation. Comp Biochem Physiol Part A 152:447-469.   DOI
38 Bardet C, Vincent C, Lajarille MC, Jaffredo T, Sire JY 2010 OC-116, the chicken ortholog of mammalian mepe found in eggshell, is also expressed in bone cells. J Exp Zool 314B:653-662.   DOI
39 Briesemeister S, Rahnenfuhrer J, Kohlbacher O 2010 Going from where to why-interpretable prediction of protein subcellular localization. Bioinfor 26(9):1232-1238.   DOI
40 Cho EJ, Choi CH, Sohn SH 2005 The amount of telomeres and telomerase activity on chicken embryonic cells during developmental stages. Kor J Anim Sci 47(2):187-194.
41 Cordeiro CMM, Esmaili H, Ansah G, Hincke MT 2013 Ovocalyxin- 36 is a pattern recognition protein in chicken eggshell membranes. Plos One 8(12):e84112.   DOI
42 Gautron J, Hincke MT, Mann K, Panhe'leux M, Bain M, McKee MD, Solomon SE, Nys Y 2001 Ovocalyxin-32, a novel chicken eggshell matrix protein. J Biol Chem 276:39243-39252.   DOI
43 Dacke CG 2000 The parathyroids, calcitonin, and vitamin D. In: Whittow GC (Ed.), Sturkie's Avian Physiology. Academic Press. New York. pp 473-488.
44 Davis RA 1997 Evolution of processes and regulators of lipoprotein synthesis: from birds to mammals. J Nutr 127: 795S-800S.   DOI
45 Flemström G, Allen A 2005 Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin. Am J Physiol Cell Physiol 288(1):1-19.   DOI
46 Gautron J, Rehault-Godbert S, Pascal G, Nys Y, Hincke MT 2011 Ovocalyxin-36 and other LBP/BPI/PLUNC-like proteins as molecular actors of the mechanisms of the avian egg natural defences. Biochem Soc Trans 39(4):971-976.   DOI
47 Griffin HD 1992 Manipulation of egg yolk cholesterol. a physiologist's view. World's Poult Sci J 48:101-112.   DOI
48 Hill AT, Eissinger RC, Hamilton DM, Patko J 1980 Sample sizes required for predicting albumen quality in stored eggs from eight commercial stocks. Can J Anim Sci 60:979-989.   DOI
49 Hincke MT, Chien Y-C, Gerstenfeld LC, McKee MD 2008 Colloidal-gold immunocytochemical localization of osteopontin in avian eggshell gland and eggshell. J Histochem Cytochem 56(5):467-476.   DOI
50 Hincke MT, Gautron J, Tsang CP, McKee MD, Nys Y 1999 Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116. J Biol Chem 274(46):32915-32923.   DOI
51 Horvat-Gordon M, Yu F, Burns D, Leach Jr RM 2008 Ovocleidin (OC 116) is present in avian skeletal tissues. Poult Sci 87:1618-1623.   DOI